Talk:2012 Group 6 Project

From CellBiology

Project Discussion Page

I will be adding information at the top of this discussion page that I want all groups to read. When adding your comments please also add your signature.

This page should be used by members of your group to:

  1. Talk to each other.
  2. Distribute/Allocate research areas.
  3. Add links to reviews, articles and websites (in draft form).
  4. Add images that may be useful for your project for there to see.

--Mark Hill 02:56, 1 April 2012 (EST) There should be more than just the sub-headings on your project page by the next Lab.

Peer Assessment

  • Introduction:The information is well explained, and the paragraphs are short and straight to the point. A picture in this section would make the page more eye-catching. Lacks referencing.
  • Structure: Good choice of content included. Perhaps add in a diagram of an isomer for comparison. The picture needs a caption.
  • History: Well researched, however next to each discovery should be the name of the researcher and where possible their research article.
  • Insulin Receptor: Well explained section and a good choice of diagram to illustrate the information.
  • Pathway: The information is well written, however it could be better interpreted with a listed format. Great diagram to illustrate the written information.
  • Normal Function: This section is well written and clearly structured. It lacks references.
  • Abnormal Function: The information is very clear, however I feel that it could be better interpreted in a table format. Pictures could make this section more interesting as well.
  • Current Research: Very well written. It would be nice to have links to the research articles for further readings.
  • Overall impression: I enjoyed reading through your page. For the most part the information is clear, interesting and thoroughly researched. Pictures and coloured tables would make the page more appealing and eye catching.

I really enjoyed reading the "signalling pathway" section. It was clearly set out, with good use of subheadings. The way the text closely related to the image also helps new readers to follow along easily. I also thought the "abnormal function" section was well set out. A few images would make it more helpful for readers.

Some improvements that I think can be made include adding a page heading with the signalling pathway, before "introduction". I was reading "Introduction" and I wasn't sure what exactly your page was focusing on. I'm guessing it's insulin signalling, in which case I don't think the content on abnormal and normal function belongs in the intro; they should be brought up in separate sections later on (which you do have). Having said that, I think something like "structure" does belong here, if you're not sure of what additional content to add. Another point to note is that you should add a caption to the image and reference it; the same comment goes for all your other images.

The "history" section needs expanding - how does the discovery of things like GSK3 and PI3K relate to the advancement of our understading of insulin signalling? It's not detailed enough to be relevant.

The "insulin receptor" section is awfully empty. Furthermore, the way the content is written didn't really engage me - it sounds very monotonous. Additional things I would've expected to learn about in this section would've been along the lines of what gene(s) code for it, and since your image depicts it, what the role of the juxtamembrane motif, ATP binding site, regulatory loop, and C terminus do.

There was a prominent lack of referencing in "normal function". Did you refer to one source only for all that information, or did you just not bother to acknowledge the rightful authors?

Finally, I noticed you have three images on your page (there should be four members in your group), and two of them don’t provide citations and two of them again don't provide copyright information. Did you get them straight from wikipedia? Note that we're only allowed a maximum of one wikipedia image.

I hope these comments are helpful in pointing you toward what can be improved.

  • Pros
    • Content correctly cited and referenced
    • Good use of subheadings; makes information more easily readable
  • Cons
    • Needs a main heading for the actual page titled “Insulin Signalling”
    • No copyright information for images under “Structure of Insulin” or “Signalling Pathway” sections
    • “History” section is lacking citations
    • Page needs more breadth of research, especially the “Introduction” and “Normal Function” sections
    • No student-drawn image
    • Maybe add text to the external link to YouTube under “Insulin” section of the Introduction so readers know it’s a video (could be confused for just a citation)

  • I don't think intro should have so many subheadings when under each there isn't much info, or many subheading size it too large?! But content is good, easy to read, simple nice work.
  • Structure of insulin section- it has a nice big photo which is eye catching but i have a few issues with it- NO copyright or info in regards to where photo came from. Nothing to say what green and purple is representing, no text to accompany image so i really don't know or believe what I'm looking at. Is purple insulin or the green or both? Its confusing. The specifications are good but surely there is more to say about this molecule.
  • History is poor, for example "1988 Discovery of PI3K" i don't understand what this even means!, i don't see a reference for me to look into it. seems like this section was done in ten mins, i think if you spent more time you could have a great intro i think insulin is so well known that there would be multiple reviews about its history and it just takes some initiative.
  • Insulin receptor: is good, clear, succinct. But i really don't think the image needs to be so massive..
  • WOW signalling pathway section is really really good, i actually read the info, theres not too much that its a drag to read and there is an image that relates. its a perfect balance of information to image.
  • Normal function section is good, has potential to be better by adding some figures, images, graphs. EG, pancreas islet cell image from histology slides from HISTOLOGY, figures of levels of insulin after food before, such things found in many books...
  • Abnormal section has good info but nothing to make me what to read it, think of adding some diagrams or graphs or something to engage your audience

Week 9 Discussion

Hey Guys, great work. Thanks for doing the work you were assigned to do. I noticed that we have not included all the pictures and information from our online google document, so if anyone can do that, it will be very nice as it does not seem I have access to it anymore. There is a problem with referencing at the end, it seems that the same reference is added over and over again, please advice me if any of you know how to fix that problem. And please include more photos please.

Could we from now please carry out our conversation here, and not in google documents cause i cant access it anymore thanks

Z3333427 12:31, 10 May 2012 (EST)

General Discussion

Hi guys, as we previously discussed, I think we should continue to discuss the different subtopics under each heading. That way it would be much easier to locate the work and organise the page. Although we have allocated research areas, I believe we should all contribute to assist each other if we came across each other's resources.

Z3333427 10:13, 13 April 2012 (EST)

Hey guys, i have been having problems with referncing this website, ever since we changed our referencing format, if anyone could explain how to format this website, it would be kindly appreciated.

Overall Structure


Structure of Insulin


Hey guys, this is a great article bout the history of Insulin, and includes a timeline. But i don't know if we can site it..


hey guys can we please expand on the history a little bit please...just say how it was discovered...brief paragraphs.

Insulin Receptor


Signaling Pathway

When Insulin binds to the receptor, the insulin receptor subtrate results in the tyroisine phosphorylation of the insulin receptor tryosine kinease substrate (IRS) by the insulin receptor substrates (IRS). As a result, the association between IRSs and the regulatory subunit of phosphoinositide 3-kinease (PI3K) is enabled. In turn, PI3K activates 3-phosphoinositide-dependant protein kinease 1 (PDK1), which in turn activates a serine kinease (Akt).

Furthermore, the Akt deactivates the glycogen synthase kinease 3 (GSK-3), and thus activates glyocen synthase (GYS), and in addition to that, glycogen synthesis. The Activation of Akt in turn leads to the translocation of GLUT 4 vesicles to the plasma membran from their intracellular pool, where they accelerates the uptake of glucose into the cell.

<pubmed>6381509</pubmed> This is an article from 1984 that describes activity of tyrosine kinase linked insulin receptor. It can help to outline the basic steps in insulin signalling.

Z3333427 10:41, 13 April 2012 (EST)

<pubmed> 11742412 </pubmed> good secondary journal article summarizing signalling.

Heyy i found an article [Insulin Signaling in the Central Nervous System A Critical Role in Metabolic Homeostasis and Disease From C. elegans to Humans] <pubmed>5855309</pubmed> --Z3291200 09:57, 3 May 2012 (EST)

Normal function

( i was just wondering, normal function, isnt that the same thing as signalling pathway, whereas abnormal signalling is where i mention what could go wrong with the signalling pathway???) - or do i talk about alternative pathways, if there are any??

Z3333427 10:43, 13 April 2012 (EST)

Ya that's true -- i think we should talk about abnormal pathways separately and alternative pathways separately. --Z3333794 12:41, 16 April 2012 (EST)

Abnormal Function

Abnormal function can lead to diseases such as Alzheimer's disease due to deficient brain insulin signalling pathway. it can also be the cause of silent coronary artery disease, as well as diabetes (type 1 and 2).

if anyone have any information that Discusses the abnormality in the signal pathway which leads to these diseases. - please post beneath. thank you

Z3333427 10:47, 13 April 2012 (EST)

Abnormalities of myocardial insulin signaling <pubmed>19897715</pubmed>

<pubmed>19797329</pubmed> (type 2 diabetes and left ventricular dysfunction

--Z3284634 10:58, 3 May 2012 (EST)

Current Research


Possible references for Group Project (Group 6: Insulin Signalling)

<pubmed>9566965</pubmed> The article uses pulse-chase labelling technique to follow the steps involved in the maturation process of the IR protein. This will make it more clear how the structure of the IR forms and binds to insulin.

<pubmed>1500426</pubmed> This article describes the structure and function of tyrosine residues on the insulin receptor.

<pubmed>8376461</pubmed> This article provides with information on different receptor domains required for different internalisation steps.

<pubmed>6381509</pubmed> This is an article from 1984 that describes activity of tyrosine kinase linked insulin receptor. It can help to outline the basic steps in insulin signalling.

--Z3333794 18:05, 28 March 2012 (EST)

Journal article for group 6

Lab question 2

1)<pubmed>7540611</pubmed> This article investigates the stimulation of 3T3-L adipocytes with insulin. It investigates the involvement of activation of tyrosine kinase for insulin signalling

2)<pubmed>10459021</pubmed> The primary article investigate the effect of nsulin-like growth factor (IGF) II and its over expression in human cancers.

3)<pubmed>7040416</pubmed> The study of this article provided evidence about the internalization of insulin in hepatocytes.


Z3333427 21:21, 28 March 2012 (EST)

Insulin Signaling

1). <pubmed>15576463</pubmed>

This article discusses the inhibition and activation of insulin signalling through activating / inhibiting rapamycin pathway and in turn, the activated, inhibited receptors.


The possibility of enhancing the activity of insulin through insulin degrading enzyme is discussed in the review article. This is to research further into the different pathway insulin signalling affects and how it is carried through.

3). <pubmed>22046260</pubmed>

Insulin Growth factor signalling through insulin isoforms that contribute to breast tumors/cancers - allows a deeper understanding into the results of altered insulin receptor expression.

4). <pubmed>22276152</pubmed>

Muscle reactions to insulin receptor signaling and the uptake of glucose during endotoxemia, stimulated by insulin. --Z3284634 15:44, 29 March 2012 (EST)

Insulin Signaling

1)<pubmed>21498522</pubmed> I'm not sure if we can use this review as a reference but it covers basic details about insulin; what it does, its receptors, how is regulated and it's signaling pathways. So I thought that it will be good for just background information.




5)<pubmed>21448434</pubmed> This is not really about Insulin signaling but Insulin degrading Enzyme. I thought that because this mentions 'Alzheimer's disease' we might talk about it in abnormal section?

--Z3291200 16:10, 29 March 2012 (EST)