Talk:2012 Group 4 Project

From CellBiology


--Mark Hill 11:38, 4 April 2012 (EST) As of Wed 4th April (week 5) other than the requested individual assessment references there is nothing on either your project page (other than the template sub-headings I provided) or discussion page (reference to Facebook page for work allocation) that indicates that any work has been carried out on this project. I am happy for you to use other media in your discussions, but remember my assessment will be based upon what appears on your Project/Discussion page. I remind you of the assessment criteria and my expectation of ongoing demonstrable contributions.

--Z3287603 15:11, 22 March 2012 (EST)


PEER REVIEW

  • Introduction: Easy to understand and a good outline for the rest of the page.
  • History: The table is well designed. The information is simple and well explained which shows a good understanding of the content (as opposed to paraphrasing complicated text). It is important to include the full name of the researchers (eg. Who is 'Lewis'?) and where possible links to the research articles. Surely there have been further discoveries since 1995.
  • Pathway: This section is well explained and I liked the summary paragraph. A flowchart would be ideal here; this would be a good place for the hand-drawn diagram.
  • Proteins and Receptors: I like the simple layout of the text, however more information is required. Perhaps set up a table to compare the structure and functions of each component, and highlight their importance to the pathway. The picture is really good, however I feel that it is more relevant for the 'Pathway' section. References are needed!
  • Normal Function: I don't feel that the specific role of Notch in CNS & cardiac development was well described. You have a really great flowchart illustrating the role in CNS development, so perhaps you should explain what is happening in that picture. This was done better in the role of Notch in kidney and pancreatic development, where you went into detail of what the Notch pathway is responsible for and the background information on how these discoveries were made.
  • Overall Impression: I really enjoyed reading the information on this page. The explanations are clearly written in your own words and are easy to follow and understand. The information is well formatted into subheadings and tables which makes it easy to navigate around key points. That being said, there is a lot of information missing and most sections need to be expanded on (however this has already been pointed out by many others).



what was done well;

  • Normal Function section was very well written. I could understand the different roles of Notch in different tissues very easily. It gave a wide range of functions and picture is quite relevent to Notch in the CNS. This section had nice layout with the subheadings.
  • correct referencing and citations used
  • enjoyed the video

what needs Improving:

  • Your page seems rather short and many of your sections need expanding. In you intro, can you give an overview of everything you're going to talk about? The future research section doesn't appaer to have information - just the references to some literature.
  • You need a diagram for your Pathway. I find it hard to understand the text.
  • history section doesn't explain what happpened between the 30s and 1970s.

The "normal function" section is especially well done. I am impressed by the clear layout, referencing, and relevance in the picture. Together it facilitates in drawing me in and engaging my interest. I'd like to point out, however, you're missing the reference for the figure.

Frankly, the rest of the page gives the impression of, at best, half-heartedness. I won't comment on "abnormal function", which currently does not exist. For example, the introduction sounds like an essay intro and not a wiki. This may have been an unintentional error arising from the unfamiliarity of how wiki intros are set out, and if that's the case, I suggest looking up some examples of introductions of cell signalling pathways on Wikipedia. Another example is "history". It seems awfully short to me… surely more has taken place in the way of scientific advances for notch signalling from 1917 till 2012? Like what about the gap between 1930-1978 and also 1980-1995? Additionally, I think the image linked under "proteins and receptors" would be more appropriate under the "pathways" section. Instead, images depicting the gene sequences or even the 3D crystal structures should be included under "proteins and receptors".

Just another suggestion is beginning your wiki page with the name of the signalling pathway you are talking about. That way it's clear from the beginning what you are writing about.


  • Pros
    • Content correctly cited and referenced
    • Well formatted table in “History” section; makes information easily readable
    • Good use of dot points and subheadings in “Proteins and Receptors” section
    • Subheadings in “Normal Function” section made information easier to follow
  • Cons
    • “History” section lacking a bit of information and research (only 3 sources referenced)
    • “Pathway” section hard to follow (maybe include flow diagrams of signalling pathway? Possibly student-drawn?)
    • Images under “Proteins and Receptors” and “Normal Function” sections lack copyright information
    • Greater breadth of research needs to be conducted, reference list is not very comprehensive
    • “Normal Function” section needs proof-reading (For example, the opening three lines read: “Notch signalling pathway plays a vital role in metazoan development. Notch protein activates a signalling pathway that controls the expression of genes that are responsible for cell division, growth, migration and apoptosis. Utilization of the Notch signalling pathway determines the fate of cells in the embryonic phase.”). These sentences sound more like dot points; add “The” to the start of them or format them in dot point form.
    • No abnormal function/implications in disease section
    • No student-drawn image


  • There is no large title saying NOTCH signalling- it should be added so people know what the page is about before reading.
  • Intro is short, thats fine so long as you expand on what is said throughout the project. History is also short, i heard this signalling pathways is fairly recent and difficult to study, but maybe just a few more points will make it more comprehensive- it seems like its all based on Morgan and the fly. is there nothing else about the signalling or abnormal diseases which relate to notch signalling that can be added to your table.
  • Image of Notch Signaling Network , has no copyright info attached!
  • surely there is more known about the Protein and receptor section. I understand you don't want too much information that people don't want to read, but i think your page is on the other side of the spectrum, with not enough information in all sections (apart from normal function- which is detailed, but still easily read, contains a picture, would be nice to see a few more)
  • Videos are a good addition but i don't think they make up for the lack of effort in most sections.
  • Futher research; seems like someone just pasted a few pubmed no. in this section. at least write a sentence or two about what the article is hoping to discover etc.

-

Pros

  • good table and pictures
  • proteins involved was clear and easy to read.
  • there was a balance of writing and pictures in protein and receptor & normal func. section
  • normal function section was good, clear, good layout & info.
  • video links great for those who want further info

Cons

  • some sections were lacking more in depth info/pics when compared to other sections
  • an overall short webpage/assignment.

Possible/needed improvements:

  • what is the involvement/function of modifier proteins? More depth in their involvement ,structure of the protein if possible would be good.
  • abnormal function heading- to show a comparison of when it works and when it doesn’t.
  • a complete glossary
  • a consistent overall layout to make the whole page appealing to readers rather than some sections over others.
  • history table seems incomplete- did anything happen between 1930s-1978? 40 years unaccounted for unless nothing happened

First of all, please put down your signalling pathway as your heading. All that I can see now is the introduction - only by reading your text it becomes clear which signalling pathway you have chosen to discuss.

The introduction does not provide me with too much information as to what 'notch signalling' is. In your introduction you are supposed to give us a general overview of what you will be covering in the other sections. Most important would be to briefly mention the normal function, not solely the organs which they target. Because I did not get this information from your introduction I had a quick look at wikipedia. Now please be careful with your information! The first few sentences from the wikipedia page were very similar to what you wrote. When I looked at the reference in wikipedia and the reference you provided me with I could see that they were the same! To me this seems like you simply rearranged wikipedia text and copied their reference. I would advise you to re-write your introduction. Use wikipedia as a guideline only and give us a general (but not too general) overview of what is coming.

History looks good and is well-presented in the table. Please provide some more recent discoveries to it - I am sure some research has been done on notch signalling between 1995 and today.

The pathway sections has some good information to it, however it might make it more understandable if you put this in dotpoint form. An image/student-drawn image would also be helpful here.

It is nice and clear which proteins and receptors are involved. I did notice that there are 3 links which 'do not exist'. The picture caught my eye, which is positive. Do make sure you have the copyright statement provided.

Normal function was very expansive and had a good layout with the use of subheadings. It seems like a lot of research has been done on this section and the majority of your references are indeed listed here. When it comes to the picture, please provide us with the correct information - title and description, reference, copyright statement, etc.

Building upon function you could now discuss abnormal function, current research etc. This has not been provided yet.

It seems like you made a good attempt to start the glossary, however you could probably expand this a little. Similarly with your references, you could use a wider range of sources to get a more holistic idea of what is involved (normal/abnormal function etc). The references you do have seem to be cited correctly. --Z3333865 14:01, 12 May 2012 (EST)



I know that it has already been mentioned, but it is very important that you have a heading for your page.

The introduction seems good and succinct. A picture would be nice at the top of the page.

Using a table for the history is a great idea, but it seems rather to simplistic and incomplete. However, the referencing for the information within the table is good.

The information about the pathway is really informative and detailed. Diagrams and labelled images would be great to explain it further.

Protein receptors, Normal function and Glossary had very good information, and the use of subheadings made everything so much more organised, and easy to follow. However, I realised a number of the links did not work, and I was not quite sure as to what the problem was, something worth figuring out.

The images were did attract my eyes, and did aid me in understanding a number of the concepts.

However, what I failed to find was information about current/future research.

References seems to be correct, and overall the information is quite detailed.

Having said this, the page probably needs more expanding, as the information seems quite limited.


Group Discussion:

References to use later: Artavanis-Tsakonas, S. et. al.(1999) Notch signaling: cell fate control and signal integration in development. Science. PMID: 10221902

Das, I. et. al. (2004) Notch oncoproteins depend on gamma-secretase/presenilin activity for processing and function. J. Biol. Chem. PMID: 15123653

Dontu, G. et. al. (2004) Role of Notch signaling in cell-fate determination of human mammary stem/progenitor cells. Breast Cancer Res. PMID: 15535842

LaVoie, M.J. and Selkoe, D.J. (2003) The Notch ligands, Jagged and Delta, are sequentially processed by alpha-secretase and presenilin/gamma-secretase and release signaling fragments. J. Biol. Chem. PMID: 12826675


Oh whoops sorry guys, I made one of the images on the right as well but we'll fix it all later. Yep right now I'm working on putting much information and expanding the glossary list as I go. Just wondering if we should put a youtube clip on notch in the external link section? --Z3287603 10:34, 10 May 2012 (EST)


okay so i found research websites as some research papers which were primarily on cancer. ill put the websites here and ill put the referenced papers down below.

GRC Conference Program on Notch Signaling in Development, Regeneration & Disease

AT Laboratory Notch Signaling Programs

Pharma Strategy Blog Notch Signaling and Brain Cancer

Breast Cancer Research - i wasnt sure how to reference this page but it actually belongs below

Nature Review Neuroscience - belongs below as well

Circulation Research - belongs below as well!

--Z3289642 09:36, 10 May 2012 (EST)

so my scanner isnt working for some unknown annoying reason but the image that i adapted it from is [this] yeah youre right about getting as much info as possible and then doing the decorations later. ive been looking for some info on research ill put some of the sites that i found up underneath under papers

--Z3289642 01:50, 10 May 2012 (EST)

Totally makes sense, I think even someone without any scientific background can figure out whats happening, great job. Really? I thought left would look better, to balance the pictures out a bit. Doesnt really matter I guess, lets just work on getting all the info up first then figure out the little decorations later. oh, and i realised you can click on the thumbnail to enlarge it if you want to and click on it again for further enlargement haha silly me. --Z3290558 21:59, 9 May 2012 (EST)

Ok following on with that, I've changed the introduction bit for the 'role of notch', have a read and let me know if it "makes sense"! For the Thumb picture go onto my section and copy the command, I reckon it'll look better on the right side of the page. Excellent that you are drawing them! How are you planning to put it up? Scanning it? --Z3287603 19:37, 9 May 2012 (EST)

in regards to the 'role in notch' subheading i dont think its hard to small or hard to read dw about that bit, its better because we are making that distinction between them. also about the proteins and receptors part have you guys figured out how to do that thumb picture thing? im drawing them at present and we can put it up tomorrow yeah?

--Z3289642 18:15, 9 May 2012 (EST)


Ok I will definitely edit that bit tonight in regards to the role. I am finding great difficulty to simplyfy the function. Would you rather me to write it as a whole paragraph and not have the sub categories? --Z3287603 08:59, 9 May 2012 (EST)

Just a random thing I noticed, do you think the 'role in notch' image under the 'Normal Function' section is too small or hard to read? --Z3290558 07:51, 9 May 2012 (EST)


Also, don't forget to be adding words to the glossary! If you had to look up the definition of the word, people visiting our page probably will have to as well! Like ' progenitors' would be a good one to put in the glossary, or even if it's slightly technical, put it in. --Z3290558 10:25, 3 May 2012 (EST)

Hey the summary would help quite a lot when it comes to the function of the signalling pathway as most of the time when the papers mention certain proteins I have no clue. I agree with you in terms of completing the introduction at the end (it just makes more sense). --Z3287603 20:34, 2 May 2012 (EST)

Yeah that sounds great. I'm actually a little confused with it as well.. Also for the introduction, we should write that part last right? Once everything is almost finished so we can write a summary and brief outline of what we will talk about in our project page right??

--Z3290558 20:15, 2 May 2012 (EST)

guys im a little confused about the proteins and receptors section? shall i just put up a small summary about each receptor and protein involved ie what it looks like, where abouts its most likely located around the body, its primary function, things like that?

--Z3289642 19:22, 2 May 2012 (EST)

hey kids, this would be really useful for research so i thought id just put it up here. Notch signaling and neurogenesis in normal and stroke brain --Z3289642 21:43, 18 April 2012 (EST)

this video is amazing. have a look if you have a bit of free time its what finally made me understand notch signaling notch signaling video

--Z3289642 17:36, 16 April 2012 (EST)

So guys i dont know if you remember but raymond actually posted this that link on notch signalling pathway when we were first deciding on which pathway to use. it turns out that it comes from the same cell signalling journal that we were using for that lab a couple of weeks ago and its literally the entire pathway sorted out. check it out and let me know what you both think?

notch signaling pathway description

--Z3289642 17:28, 16 April 2012 (EST)

Random but, do you think we're allowed to reference the textbook link you put below? --Z3290558 12:24, 13 April 2012 (EST)

Cool stuff! I'm currently looking for some stuff on the history.. its so hard.... And i hope you kept the PMID for those articles for referencing, cos it should link to the actual article itself. just like we practised in referencing in class.

Heres the link to referencing: http://meta.wikimedia.org/wiki/Help:Footnotes

and btw, the textbook link is awesome! --Z3290558 10:57, 11 April 2012 (EST)


Hey guys, I've typed up some intro for my topics. I'll put it up on the main page but feel free to proof read it and let me know if I need to change anything. OH! Also check if my referencing is ok... Hope y'all have an awesome break!! --Z3287603 17:03, 10 April 2012 (EST)

Hola! I know its been difficult to find a "simpler" explanation of the notch pathway, so check out [1]. The textbook includes couple of diagrams which may help to understand the pathway. --Z3287603 20:28, 4 April 2012 (EST)


I've put the delegated roles for the research areas on our fb group page.

Check it!

--Z3290558 16:11, 29 March 2012 (EST)




NOTCH SIGNALING

Papers for Notch Signalling Pathway

1. In this article, the authors utilize the notch signalling pathway to demonstrate its importance in the development stage of Drosphila Flies. They indicate that a single mutation in notch can lead to wingless Drosophila highlighting its importance in the embryonic development stage. This article may be helpful in identifying the significance of Notch signalling.

<Pubmed>7848873</Pubmed>

2. This review article highlights the function and structure of notch proteins. This article gives an overview of the specific proteins and its signalling pathways which is useful in our group project.

<Pubmed>7950324</Pubmed>

3. In this review article, the authors examine the importance notch activity in the ”development of different organisms”. This is very useful as it allows us to understand the function of the signalling pathway not only in invertebrates but also vertebrates.

<Pubmed>8645561</Pubmed>

4. For many years notch signalling has been associated with the fate cell differentiation during the development stage of organisms however in this study the authors indicate its importance in cell proliferation and apoptosis. This is useful as it represents a fatal role in the cell cycle and mutation in the notch signalling pathway may lead to many diseases such as cancer.

<Pubmed>10528225</Pubmed>

--Z3287603 21:03, 28 March 2012 (EST)


1. Yuan JS, Kousis PC, Suliman S, Visan I, Guidos CJ. Functions of notch signaling in the immune system: consensus and controversies. Annu Rev Immunol. 2010 Mar;28:343-65. PMID: 20192807

<pubmed>20192807</pubmed>

This article reviews recent advances in terms of Notch signaling as well as tries to clearly identify Notch functions within immunological processes. It is useful in terms of getting an overview into the history of the gene as well as gaining more of an understanding into the actual pathway itself and its normal function.


2. Liu J, Sato C, Cerletti M, Wagers A. Notch signaling in the regulation of stem cell self-renewal and differentiation. Curr Top Dev Biol. 2010;92:367-409. PMID: 20816402

<pubmed>20816402</pubmed>

This article reviews notch signaling pathways at multiple stages of organismal development. It would be useful to gain a broader understanding of the pathway and how it is considered useful within the human body.


3. Radtke F, Fasnacht N, Macdonald HR. Notch signaling in the immune system. Immunity. 2010 Jan 29;32(1):14-27. PMID: 20152168

<pubmed>20152168</pubmed>

This article talks about the abnormal funtionings of notch signaling as well as current research as to how these are associated with several human disorders including hematopoiesis, lymphocyte development and T cell acute lymphoblastic leukemia. It would be useful to gain more of an insight into current research and how abnormalities in the pathway can lead to dysfunctions within the body.


4. Carlson ME, O'Connor MS, Hsu M, Conboy IM. Notch signaling pathway and tissue engineering. Front Biosci. 2007 Sep 1;12:5143-56. PMID: 17569636

<pubmed>17569636</pubmed>

This article talks about the dysfunctions of Notch signaling pathway and how it has been linked to the pathogenesis of several inherited human diseases. It describes the components within signal tranductions plus outlines the role of the pathway and finally summarises current research within the area.

--Z3289642 16:14, 29 March 2012 (EST)

Papers specifically for research

<pubmed>12496471</pubmed>

<pubmed>15817497</pubmed>

<pubmed>18497317</pubmed>