Difference between revisions of "Talk:2011 Group 6 Project"
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==Peer assessment comments==
==Peer assessment comments==
Revision as of 16:20, 18 May 2011
- 1 To do list
- 2 Peer assessment comments
- 3 chat
- 4 to do list
- 5 to do list
- 6 Post Peer Assessment Discussion
- 7 Week 7
- 8 Week 6
- 9 By Week 5
- 10 Reference list
To do list
Revised to do list based on peer assessment comments:
- Spell and grammar check
- History up until 2005
- Check all pictures are properly referenced and a link to the webpage that they came from.
- Revise headings and subheadings eg "historic researchers in NMJ"
- Discuss page layout, should we move any sections? Maybe put microscopy at the end?
- Discuss dot points for skeletal muscle etc. Some reviews loved it, some didnt. Keep as is?
- Caption current research images so people know what they are looking at
- Number mechanisms of action instead of dots as this is a sequence of events
- Format current research, too hard to understand
I think Ive covered most of the criticisms. Ive left out some conflicting criticisms where some people liked it and some people hated it, mostly these are related to too much information but im not deleting anything as we have worked too hard on it. Once you have done a dot point just put -done next to it so no-one else does it too. -Jill :)
Peer assessment comments
There a a few spelling mistakes in your page, maybe just do a quick spell check
Need to complete history section, maybe up until 2005
Some pictures arent referenced eg embryonic development
Loved the videos
Good tables to break up text
The overall page is awesome, obviously a lot of hard work and research has gone in to it. Well done guys
Group 6: Neuromuscular junction
•History anything beyond 2000?
•Perhaps remove "historic researchers in NMJ" its already under history subheading
•Video is really great idea
•For mechanism of action section can you maybe move a picture to the other left side to break up the text? It happens again latter down the page just something to think about before the final due date.
•Great use of tables and images helps to break up the text.
•Great integration of the microscopy section
•Interesting embryology research
•Clever input of potential future research with links
Group 6 Peer review
Introduction is brief and easy to understand.
History should be a little bit recent since it ends at 2000.
Mechanism of action part is mostly in dot point, less text but straight to the point and simple.
What I feel a little bit off-track is the part about advantages of light microscopy (LM) and electron microscopy (EM) utilization. I love the pictures and I feel they should be like in a gallery with a subheading “LM and EM images of Neuromuscular Junction (NMJ)”. I just don’t think the advantages and disadvantages of LM and EM are related with the NMJ.
I love the fact that you put in embryonic development of NMJ. It is so informative and shows that this is a result of a great research and for those who wants to know about how NMJ is developed, this is the page for them!
The images in common neuromuscular disorders are amazing and so eye-catching that you want to read the table!
Current and future research is very well researched with a good balance between text and image. There should be some captions under the images of current research though since they are graphs and fluorescence photos, it is hard to understand how these images are associated with what you have written.
Nice glossary and overall, this is a well researched page about NMJ. Great job guys!
I was blown away with this project page. Not only is there a high level of detail, but it is presented in such a way that doesn't overload the reader.I particularly liked the EM section, it works well because of the balance of text to the pictures presented, and is very interesting. Perhaps the mechanisms of action should be numbered because it relays to a reader a sequence of events. Numbering what occurs would just reinforce this. Also, it could be handy to add some statistics to common nueromuscular junction disoders, to give an idea of how often these diseases occur. I know these seem like nit picking, but the page is so well done I could only suggest modifying some details. Well done.
Good work guys! I really liked the video to describe the process. It really allows for a good understanding of the process! However for that section, rather than using the dotpoints, wouldn’t it be better if you used a numbering system (to show the progression of the mechanism of action?) Also I think it would be good to discuss how muscle contraction occurs (i.e. following activation of the motor neuron, the signal transduction pathway that activates the muscle and causes the contraction). Otherwise, a job well done! :)
- The first thing that I notice is that the project is very long, and clearly very thorough. This is not a bad thing, given the number of tables and images which make it much more accessible.
- Youtube videos offer a different way of accessing the information, great idea!
- There is nothing negative to say about your project, it is clear that you have worked hard and it shows.
GROUP 6 REVIEW
- You have put a lot of research into this assignment guys so firstly, well done.
- The first NMJ heading is a little unnecessary
- Introduction: Great
- History: Anything more since 2000? Also you don't need the "historic researchers in NMJ" title - it's implied by the subtitle.
- Mechanism of Action: Video is really great. There is a LOT of info here... is it necessary to go into so much detail about the motoneurons etc?
- Important Structural Component: The 'Important structural components of NMJ' heading should go next to table of contents it doesn't need so many titles. Great use of images though you need to include a link to their location from where they were found in the image's page.
- Light microscopy and EM pictures of NMJ: this section feels like it is just chucked into here. I don't think it belongs here but maybe with current research under research methods. It has great images though.
- Cellular organisation of skeletal muscle: Really well researched but i question the validity of the information here. I understand NMJ action Skeletal muscles but I think you may have gone off on a tangent and this information isn't very relevant to the topic at hand.
- Embryonic Development of NMJ: The idea of this topic is great. Awesome image though the article you explain goes into a lot more detail than necessary. I think a shorter, more succinct, summary would work just as well. Also you don't need as many images here, a caption would work better than more text and the images need copyright info and a link to the location that they came from.
- Common NMJ disorders: Don't need a title on top of the table the subheading explains it. Great images and table.
- Current Research: I got confused with the first sentence and what it was talking about. Was it in reference to the 2009 article bellow it??? Consider formatting this a little more clearly so it flows and doesn't just launch into topics. There may be a few too many images here... Maybe just one per article? Also add captions to the images so we know what they are just buy looking at them.
- Future Research: Really good.
- Glossary: Good.
- Good history, nicely detailed, but has there been anything since 2000? Surely there has.
- For the mechanism of action, I see what you’ve done with the dot points, but it could be even more effective as a sort of flow chart? That’d be really fantastic.
- A bit further down, I’m getting a bit confused with the format. I know we shouldn’t be commenting on it, but how you’ve structured your information is a bit inconsistent. Ie the dot points. If you could compile it into a succinct paragraph? Or at least elaborate a bit more into the dot points. They are good in a lecture setting (where more information is provided vocally to “fill in the blanks”) but I personally get confused without the complete sentences.
- None-the-less, you’ve got some great information there
- AWESOME table of components. Really good and helpful, especially with the addition of the pictures. I really liked that bit.
- Nice microscopy section, clever addition with some nice pictures.
- Some fantastic information in “skeletal muscle” section, but it’s not entirely applicable to NMJs. Focus more on the NMJ involvement in contraction, as opposed to the details about the structure of actin and myosin etc.
- Interesting stuff about embryology, good work there
- Nice table for the abnormalities, lots of good stuff there
- The research area looks really good, and a clever input of potential future research
- Looks like a pretty good project!
-o wow, awesome screen sized intro image.
-it is so resourceful , details are well presented in an easy understandable way.
-I have no critics for you guys.The page stood out among others ,very great effort and well done !
Group 6: Neuromuscular Junctions
Introduction: The placement of the opening picture adjacent to the contents really engages the reader. Also, the actual introduction gives a good overview of what is to come.
History: This section is concise, however some of the entries could be cleared up such as, "At the Paris conference..." The conference does not seem to have been mentioned prior, so just some rewording or clarification would be helpful.
Mechanism Of Action: The dot points are good. Maybe to make the steps clearer, you could number them.
Structure: Breaking down the structural components in a table format really makes the information accessible.
Glossary: Potentially to make it an easier read, you could put the letters above their respective entries such as 'A' above the entries beginning with 'A'.
Note: The project format and content are very good. Just make sure to look over any spelling mistakes such as in the embryonic development section, 'The growing end of motor neuron axons secret...' when i think it should be 'secrete'.
Girls, the current researches section is done,,,,,If u all come early 2mrw morning we can add up things in Intro coz i run out of English words and i dont want to write in Greek language (coz i was nearly tu submit something written in greek)....haha --User:z3255968--Rega Tabbaa 23:25, 11 May 2011 (EST)
Happy bday Jill~!!!Thanks for the embryo pic, although it's not exactly cute~~lol --Shan Ruan 20:45, 11 May 2011 (EST)
totally serious haha! im going to kill them when i see them again! i had everyone calling me saying happy birthday lol. You are too kind to think of bringing flowers! xox see you tomorrow xox
No way,,,,r u serious??? hahaha,,,i was planning to get flowers 2mrw morning...
No problem Rega! Also, my physiology friends hacked my facebook and changed all of my details, its not my birthday today haha :) Thanks though :) xoxox i will add those references now xox
--User:z3255968--Rega Tabbaa 14:32, 11 May 2011 (EST) Happy Birthday to GORGEOUS JIll!!!!!! .... I saw the emryo pic and=stunning.... I know u r busy with ur birthday but a small help for ur poor friend (me=Rega),,,, the refs for the parts that dont have,,,coz i cant. For the ATP part in mm use the biochemistry book. Just add the ref in the end for each paragraph coz i did use my words... And u know how much i memoraised for the bioc test...(hahaha)... THANKU Jill,,xx
added a cool embryo pic in development :) check it out! -Jill
hey Rega! i was working on the page all last night, i think a few of us were on at the same time for a bit haha! im going to try and do the second youtube pic now :) -did it! :D -Jill
--User:z3255968--Rega Tabbaa 23:55, 10 May 2011 (EST) Jill, i left the last bit in current research for 2mrw coz i cant open my eyes anymore... The new design was just briliant (adding the pics in both sides), I loved it....thnkUUUUU
Lizz, we needed new text. It is the same thing written twice which doesn't make sense. I am fixing a lot of things, also Jill is adding new things to balance the dot points with text.... I was trying to take the refs and add them in ur paragraphs but doesn't work,,, so a new text with new ideas needed till thursday...If u come up with smthing just added...
Oh nooo no no Rega, that's all I meant for the summary to be! I wasn't trying to take credit for your work, I'm sorry if you misinterpreted that way. I should have clarified that -- I didn't do any additional research, I just put what was already on the page in a different format because Professor Hill said the bullet points weren't okay. --Lizzy 21:59, 10 May 2011 (EST)
I saw it Shan, ThankU. OK girls, i added the ATP part in skeletal mm and there are two paragraphs now. I am trying to work on motor neuron but it doesn't save the work and ill try it later.... --User:z3255968--Rega Tabbaa 21:28, 10 May 2011 (EST)
Hi guys, I've just added another 5 points into the history area, including the one Mark Hill was talking about where synapse will still form in the presence of basal lamina but absence of myofiber. --Shan Ruan 20:38, 10 May 2011 (EST)
Hi Rega!! i only just read your message, can i meet you at WW at 11am? or anytime between 11 and 1? - Jill
Jill, i need ur help for the pics for current research section. 2mrw ill be early morning at WW. Each pic has a paragraph so it'll look good. I'll let for 2mrw to take off things that are repeated in mech.function coz doesn't make sense. For other points i did fix it by adding new text and letting some dot point.. eg, the structure is as follows,,,,..The developing part is good, and know with the pics is completed.. If u want to add the pics from the home just click the ref and choose what pic u want.... --User:z3255968--Rega Tabbaa 00:03, 10 May 2011 (EST)
Jill, i added things in SC, Mit, BL,, and ALSO fixing the the repetition that Lizzy done to my points. Lizzy, it is unfair to repeat me and say that u done the work. U should do things that are not mention at all such as function, current research, future research.... ANYWAY, girls i did put new text and the structure for some topics are still in dot points, and also ill stay for a long time 2night to work on other parts that need to be fix...
Jill you are amazing. Thanks! --Lizzy 17:48, 9 May 2011 (EST)
GUYS!!!! Ive manually changed ALL the pubmed references and referenced the page properly instead. The page now loads and saves STRAIGHT AWAY!! :D IM SO HAPPY!!!!! -Jill
hey guys, when you want to post something in this page, just edit that section, then it wont take ages to save :D
Hi Liz! I agree with your point about it being repetitive, but i think having both up helps people understand better. eg i read better in sentences, and others may read better in dot points. Lets leave it for now and see if anyone mentions it in the peer review :) Thanks heaps for doing that! Shan: did you add the section on development? Its really really good! i added it to the project page. i hope thats OK.
Yep I did, and thank u for adding it onto the project page, couldn't do that at home :) --Shan Ruan 11:41, 9 May 2011 (EST)
Im going to spend the next few hours to try to re reference as much as i can, so the page doesnt take so long to load each time. Hopefully it helps :) - Jill
Hi guys, I've added body to "current research, future research & history" and I've also done some editing & summarizing. When we last talked in class we agreed to keep the bullet points and summaries, but have a look, it sounds a little repetitive to have both. Should we eliminate one? --Elizabeth Bergman 21:10, 8 May 2011 (EST)
I've summarized most of the first half of our project page, and I'll be doing the rest of the weekend & adding it below the dot points like we discussed. I'm having some trouble uploading now but I'll keep trying. I've also added to current/future research --Elizabeth Bergman 20:09, 6 May 2011 (EST)
to do list
Hi guys, I've added body to "current research, future research & history" and I've also done some editing & summarizing. When we last talked in class we agreed to keep the bullet points and summaries, but have a look, it sounds a little repetitive to have both. Should we eliminate one? --Lizzy Bergman 21:10, 8 May 2011 (EST)
I've summarized most of the first half of our project page, and I'll be doing the rest of the weekend & adding it below the dot points like we discussed. I'm having some trouble uploading now but I'll keep trying. I've also added to current/future research --Lizzy Bergman 20:09, 6 May 2011 (EST)
-referencing. -done -Jill --z3265772 20:27, 9 May 2011 (EST)
-finish history i think we need to do more than this, either by adding more points or expanding the points at the bottom
-more on development I've done more on development as adviced by Rega.--Shan Ruan 16:47, 30 April 2011 (EST)
-current research -- I've added some body to this --Lizzy Bergman 21:10, 8 May 2011 (EST)
-future research -- I've also added to this. Someone added an article about Parkinson's disease that I'm not sure is properly referenced or relevant. I didn't want to delete it in case you wanted to use it elsewhere, but have a lookie --Lizzy Bergman 21:10, 8 May 2011 (EST)
-external links not essential, and ive added some anyway. Add more if you find some - Jill
-glossary- mostly finished this today --Jill De Coster 09:49, 29 April 2011 (EST)
-spell check entire page
-expand on dot points - mark mentioned dot points are insufficient to explain the process properly :(
-more on introduction
Hi all, apologies for my lack of contribution over mid semester break. I was travelling and had no access to internet. However I've been reading a few articles and I'll add further information where I can & build on what you all have already. It looks fantastic! Very cool videos you posted, Rega. Looking forward to seeing you all this week. --Lizzy 23:11, 2 May 2011 (EST)
Rega, i posted it for you, the stupid page is being very temperamental, sometimes i can save, sometimes i cant :( i might give up until next week because its taking up to much of my time --Jill De Coster 11:27, 29 April 2011 (EST)
Still, im trying to add and save the text and is not doing it... so, the text that i wanted to add in Current researches will be here till the pbl with the page will be fixed..I can't save anything on our page...
Neurexin in Embryonic Drosophila Neuromuscular Junctions (2010): In this study, Chen et al unfolded the role of the neurexin in Drosophila glutamate receptor abundance. Their results suggested that neurexin in embryos is present both pre and postsynaptically. Presynaptic neurexin promotes presynaptic active zone formation and neurotransmitter release, but along with postsynaptic neurexin, also suppresses formation of ectopic glutamate receptor clusters. During the research several methods were used. Starting with the hybridization where embryos were manually staged and dissected to examine neurexin expression. Dissections were performed as for confocal imaging and electrophysiology. For electron microscopy, genotyped Drosophila eggs were dechorionated with bleach. 
Muscle Mitochondrial Uncoupling Dismantles Neuromuscular Junction and Triggers Distal Degeneration of Motor Neurons (2009): Dupuis et al looked at abnormalities of the mitochondrial energy metabolism. Their findings proved that this defect is sufficient to generate motor neuron degeneration and suggest that therapeutic strategies targeted at muscle metabolism might prove useful for motor neuron diseases. Furthermore they suggested that muscle selective alterations in mitochondrial function might initiate NMJ pathology and participate in triggering motor neuron degeneration in ALS. Biochemical measurements were carried out followed by electromyography recordings and PCR analysis. 
Jill, i couldn't open the page 2-8pm, it's so slow now and i don't know why... I'll try to put some text to the current research not all... I want to see what Shan + Lizzy are doing or what they will??
What do u think about Schwann Cells, mitochondria and BL.... I think that they are important, they are components of NMJ.... How can i put 2 more rows in the first table?? I'v the material ready for it and the pics too.. Ok-have a nice break...--User:z3255968--Rega Tabbaa 21:36, 28 April 2011 (EST)
Did the glossary :) off to work now xox --Jill De Coster 15:59, 28 April 2011 (EST)
to do list
-referencing. -wait until next lab, no references will save
-more on development I've done more on development as adviced by Rega. I can't edit the main project page at all, tried so many times, I think the server is down? So I'm just sticking the ref here for now.--Shan Ruan 16:47, 30 April 2011 (EST)
-glossary- mostly finished this today --Jill De Coster 09:49, 29 April 2011 (EST)
-spell check entire page
-expand on dot points - mark mentioned dot points are insufficient to explain the process properly :(
Hi Rega, im temporarily giving up on the page for today, ive spent a long time referencing but nothing will save. ive emailed Dr Hill and he says he is fixing it, so i think ill wait until tomorrow :) --Jill De Coster 14:28, 28 April 2011 (EST)
Hey Rega!! LOVE the videos!! That was such a great idea to include them! i have added them to the page, so i dont think we need to add anything else for that section :) i am going to finish referencing today, and then maybe start future research. Do you like the microscopy section i added? :) - Jill --Jill De Coster 13:36, 28 April 2011 (EST)
Hi guys, there have been some problems saving stuff on the page, please please please dont edit the table under the heading Common neuromuscular junction disorders. i spent 6 HOURS trying to fix it! :O i never want to see that table again! haha :) Jill --Jill De Coster 13:15, 28 April 2011 (EST)
Jill watch this video about the sliding filament during mm contraction, watch the sarcomere.. 
Actin/Myosin Movie 1 
Actin/Myosin Movie 2 
If we put the Actin/Myosin both of above, we don't have to write how they work..
Hey Jill,,, im not going to upload my pic because urs is really good. Can we get rid of the other pic(troponin, myosin & tropomyosin)? About ur Q, I think that Dr Mark was more interested in cells that make NMJ. However, I'd the material ready for it and I thought was better not to uploaded. OR, maybe we can put a video for it.... Second, I put all my refs in the discussion page and if u remember i told u that we do it together, but u done it all. OMG, i know it takes so long to do the refs (i tried it with the history part)and THANK YOU. Organise one day next week and ill sit with u to give the final look of our page... --User:z3255968--Rega Tabbaa 19:43, 27 April 2011 (EST)
does anyone think we need to include excitation contraction coupling anywhere?--z3265772 14:41, 27 April 2011 (EST)
note to all: PLEASE reference anything you put up so people can see where you got your information, i have just spent two days trying to find where your information is from so i can reference it all :( --z3265772 10:52, 27 April 2011 (EST)
Rega: I think the clinical manifestation and treatment is pretty important, and it shows we have done more research. i have almost finished this section, and wanted to upload it today. The more we have on our page (that is relevant) the better, because it shows we have done more work. what do you think? if you still dont like it i will remove it, but i spent a lot of time yesterday researching these :( I think the muscle picture is a great idea, ive been trying to find a good one. if i find one i will upload it today and then see if you like it. I think reducing some text and having more in a picture is a perfect idea :) --z3265772 10:18, 27 April 2011 (EST)
--User:z3255968--Rega Tabbaa 01:41, 27 April 2011 (EST) Jill, i'm trying to draw a pic that includes and the sarcomere, so maybe we can get rid of some text and put a pic that talks about everything,, im still working on it. Let me know what u think..... The other thing is about the 2nd table. I think maybe we should not write about clin.manifestations and treatments,,, so the second table must enclude only three colons: the disease, the link with NMJ and the pic.... What do u think??
Thanks for doing the tropomyosin Rega! i thought i did it already, but it must not have have saved properly sorry --z3265772 22:26, 26 April 2011 (EST)
Jill, i liked it. Yesterday night, i really thought that a table or diagram will attract more eyes in looking at our page...Histology book had good pictures of BL and ill see what i can do... Don't 4get some dot points for the tropomyosin... --User:z3255968--Rega Tabbaa 18:44, 22 April 2011 (EST)
i have taken this out of the first table for now, until i can find a good picture and rewrite in lamens terms :)--z3265772 16:55, 22 April 2011 (EST)
|Basal lamina (BL) |Surrounds the Schwann cells of the axon and muscle fibre, Projects into folds that enclose the postsynaptic membrane |Layer of extracellular matrix, Synaptic cleft is filled with dense BL, Contain ACh-esterase, Lamina densa comprising the intermediate part of BL, procollagen type 4 is the most important component 
Jill, i found this in uTube,, pls have a look.. I am adding some work that I've done for the cell components of NMJ.
--Shan Ruan 22:47, 20 April 2011 (EST) Hi guys, I've added some contents to the clinical manifestations for the table. One question though, should we use jargons like "ptosis" instead of "drooping eyelid" or the other way around to make the content more accessible?
--Lizzy 13:14, 20 April 2011 (EST)
Hi ladies, I've added a few images that could provide some visual material:
I wrote something about the ALS-motor neuron disease.
Kumar, V., Abbas, A.K., Fausto, N., & Aster, J.C., (2010). Robbins and Cotran. Pathologic Basis of Disaese, eighth edition. Saunders Elsevier 2010.
Muscle Mitochondrial Uncoupling Dismantles Neuromuscular Junction and Triggers Distal Degeneration of Motor Neurons. This research explores that mitochondrial defect is sufficient to generate motor neuron degeneration. Furthermore, it suggests that therapeutic strategies targeted at muscle metabolism might prove useful for motor neuron diseases.
Hey guys, just wrote the introduction in the way how Dr Mark want it. This intro is not the final.
Development of NMJ
--Shan Ruan 01:01, 14 April 2011 (EST) Hey guys, just wrote sth for this section. Sorry I came back from a conference in Hobart just yesterday, thus didn't have much time to provide more input.
--Lizzy 19:22, 13 April 2011 (EST)
Pretty much finished up the table, just working on find an image for CMS. Found a bunch of great ones that are copyrighted. Boo. See you all in prac tomorrow!
awesome! Thanks Lizzy! --Jill De Coster 13:35, 11 April 2011 (EST)
--Lizzy 12:37, 11 April 2011 (EST)
Hello hello, I've added to the table on common disorders. Descriptions, treatments, and images for Botulism, CMS, and Myasthenia gravis.
Motor End Plate
- Specialised region of the sarcolemma
- Highly folded
- Holds a high concentration of AChRs
- Also called Myoneural Junction (Junqueira C.L. & Caraneiro J., 2005. Basic Histology, text & atlas, 11th edition. McGraw-Hill Companies.)
- Receive neurotransmitters in order to propagate an Action Potential
- Responsible for the terminal tree like branching of a motor axon on a muscle fibre
- Maintains muscle tone through stretch reflex
- Efferent neurons
- Originate in the ventral horn of the spinal cord
- Synapse with muscle fibers
- Carry information from the central nervous system to muscles
- Facilitate muscle contraction
- Somatic once are directly involved in the contraction of skeletal muscles
- Influenced by input descending from the brain
- Affected by a class of diseases known as motor neuron diseases
- Amyotrophic lateral sclerosis
Messenger RNA Oxidation Occurs Early in Disease Pathogenesis and Promotes Motor Neuron Degeneration in ALS 
For Jill- neuromuscular junction disorders http://en.wikipedia.org/wiki/Myasthenia_gravis http://en.wikipedia.org/wiki/Lambert-Eaton_myasthenic_syndrome http://en.wikipedia.org/wiki/Botulinum_toxin http://en.wikipedia.org/wiki/Botulism and for an overview on all http://www.merckmanuals.com/home/ag/sec06/ch095/ch095c.html --z3265772 12:35, 8 April 2011 (EST)
ive uploaded a picture that Rega drew for the main picture, i think its a really good overview of our topic so ive put it at the top. Eventually as the page gets bigger, the contents menu will grow and the picture will fit the page properly (as in my embro project i did). Hope thats OK! we have four weeks until we need to have finished our page for peer review :) -Jill --z3265772 09:10, 8 April 2011 (EST)
Group 6, now we have plenty of research articles and books in our hands, so start writing things down. Then we can link our writings together and design our page. Thank you Jill for helping me to upload the drawings.
Functional anatomy of the NMJ
- Nerve impulse reaches the motor nerve terminal
- Specialized proteins forming ion channels in its cell membrane open quickly
- Calcium enters into the axon terminal
- Synaptic vesicles are filled with ACh which is attached to special site of it
- Ca causes some of the vesicle membranes to fuse with the nerve terminal membrane
- ACh content is released into synaptic cleft
- ACh diffuses rapidly across the gap and binds to the ACh receptors (AChRs)
- When this binding occur, small positively charged sodium (Na) ions enter the muscle
- This lead to the depolarization across membrane
- End-plate potential in turn opens the voltage-sensitive Na channels at the synaptic fold
- An “all or nothing” action potential starts
- Propagated along the muscle fibre in each direction
- Initiation of a muscle contraction occur
- Then, the AChR pore closes
- ACh unbinds and broken down
- The space between the axon terminal and the motor end plate
- Separates the presynaptic from the post synaptic cells
- The electrical signal cannot cross
- Membranous organelle
- Contain neurotransmitter Acetylcholine (Ach)
- Found within the axon terminals of neuron
- Transmitter release occurs by fusion with the presynaptic membrane
- Ach is liberated by exocytosis into the synaptic cleft
- Cholinergic neurons
- Skeletal muscle movement
- Regulation of smooth muscle
- One of the principle neurotransmitters of the peripheral nervous system
- Released by a motor neuron at the NMJ
- Bind and activate a receptor protein
- A very effective deliverer of sodium ions, which stimulate muscle contractions and excites nerves
- The enzyme acetycholinesterase (AChE) hydrolyzes acetylcholine into acetic acid and choline
- Choline travels back to be recycled into acetylcholine and start the process over again
- Concentration of ACh remains higher if the AChE is inhibited
- AChE inhibitors delay the degradation of acetylcholine
- This inhibitors are used to reverse muscle relaxants and sometimes to treat Alzheimer's disease
INTRODUCTION (anything regarding the introduction section of the project page goes under this heading pls!)
Just added a general description (will add reference later).--Shan Ruan 22:56, 6 April 2011 (EST)
Just an idea, as this page fills up with an endless amount of information, it takes ages to edit and save the page. To overcome this problem, just add a heading every now and again like i have done above, then we will just need to edit this heading, and the page saves quickly.
Also, we seem to be adding information to this page that could go straight on to our project page. We can always edit and refine later. I think it would be a much bigger job to eventually copy from this page on to our project page when we want to finish it. We will also lose alot of information if we do that because it will get lost on here. Maybe keep this page for chatting/adding relevant articles and if you have good info on something, add it straight on to the project. what do you guys think? --z3265772 22:22, 5 April 2011 (EST)
I think both are good ideas (about adding new headings and about contributing directly to the project page). It would be easier to delete info we don't need rather than semi-frantically add to it at the end. I'll start posting there more often. --Lizzy 18:14, 6 April 2011 (EST)
--z3265772 22:13, 5 April 2011 (EST) Hi guys, im going to kill two birds with one stone. i wanted to do this weeks exercise and fill in the relevant heading as i go. so i found some stuff for future research: http://www.ncbi.nlm.nih.gov/pubmed/20955113 it is about future stem cell research for neurodegenerative diseases. I added it straight on to the project page. For some reason i cant reference it though, so ill ask mark about that next week :)
--z3265772 22:13, 5 April 2011 (EST)
--z3255968 10:02, 5 April 2011 (EST)
Regas awesome drawing
Post Peer Assessment Discussion
Hey guys in response to the peers i moved around my pictures, gave them text under them, and added a lot of new information and some photos to the structure section. the only issue we have left is to clean up our reference list since it mostly looks like a bunch of pubmed IDs. i guess we will ask mark what to do with this.--Michael Orenstein 21:48, 25 May 2011 (EST)
- This is the week before the mid-session break.
- In the lab this week we will have an opportunity to discuss any issues which are slowing progress on your project.
- The Thursday of the week beginning 02 May will be when all projects will be open to Peer Assessment.
- What you have on your page by Thursday of that week will be the content that others in the class will comment upon.
- I see many groups now have subsection titles for their projects.
- Here are some searches: Pubmed search all databases junction | PLoS junction | JCB junction You can now simply put your own search term into each top window.
- Now's the time to get your images, movies, media etc uploaded. Biomed central | JCB | JCB Archive | PLoS. Once uploaded you can make a gallery on either your project or discussion page using <gallery>File:name here</gallery> tags with your image files listed between the tags. When you upload project images, add this text as it appears replacing # with your own Group number to the summary information [[Category:2011Project#]].
- Shown below are the criteria that will be used to assess your final project.
Group Assessment Criteria
- The key points relating to the topic that your group was allocated are clearly described.
- The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
- Content is correctly cited and referenced.
- The wiki has an element of teaching at a peer level using the student’s own innovative diagrams, tables or figures and/or using interesting examples or explanations.
- Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
- Relates the topics and content of the Wiki entry to learning aims of cell biology.
- Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer’s wiki.
- Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
- The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
- Develops and edits the wiki entries in accordance with this sites wiki guidelines.
By Week 5
Each Group member has added to the discussion page:
- A Review Article
- A Historic Research Article
- A Current Research Article
No two students should add the same paper and there should be a link to the original article.
--Mark Hill 17:18, 30 March 2011 (EST) OK Group 6, You and Group 5 are the only groups that have begun your project work, well done! Though I can only see contributions from Z3255968 and Z3265772. This search should get you started with the references Search Pubmed: Neuromuscular Junction
Hi Guys, Here are the articles I've found pretty useful to NMJ.--Shan Ruan 13:32, 5 April 2011 (EST)
- Review Article: Neuromuscular junction in health and disease This is a review article from the British Journal of Anaesthesia. It outlines the structure and function of the NMJ and (discusses the anaesthetic implications of disease of the junction)<- can ignore this part. Current concepts in neuromuscular transmission Outlines the general anatomy and physiology of NMJ. Both articles overall are very useful but you need to ask for permissions to reference them. However, since they are review articles, there should be plenty of articles down the reference lists.
- Historic Research Article: The Receptor Tyrosine Kinase MuSK Is Required for Neuromuscular Junction Formation In Vivo This research article indicates the role of the receptor MuSK protein which responds to a critical nerve-derived signal (agrin), and in turn activates signaling cascades responsible for the formation of neuromuscular synapses.
- Current Research Article: The Function of Cortactin in the Clustering of Acetylcholine Receptors at the Vertebrate Neuromuscular Junction This research article suggested a novel function of phosphorylation-dependent cortactin signaling downstream from agrin/MuSK in facilitating AChR clustering at the developing NMJ.
- Hi guys, Lizzy here.
I've been surfing around Pubmed. Here's a recent article on the significance of the role of MRF4, a transcription factor that may be involved in maintenance of neuromuscular junctions: Maybe we could mention it briefly under "Current Research." Also, this article had some lovely images: However I'm not sure how relevant the article is, and I'm also not sure if we could get permission to use the images on our page. But maybe they'd be cool to have a look at on your own time. --Lizzy 16:46, 4 April 2011 (EST)
- P.S. Also, if you scroll down to the bottom of the PubMed Copyright notice page, there are 3 websites listed whose material we can use without special permission as long as we cite sources. Maybe they'd be handy for some neat images or background info.
- The three articles to be found by the end of Week 5:
- Review Article: On the development of the Vertebrate Neuromuscular Junction
- Historic Research Article: Change in the Fine Structure of the Neuromuscular Junction of the Frog Caused by Black Widow Spider Venom This was published in the JCB by Clark, Hurlbut & Mauro in 1972. It's a super interesting article! Heads up though, it's a big file.
- Current Research Article: ARIA, a neuromuscular junction neuregulin
--Lizzy 17:21, 4 April 2011 (EST)
Group 6, this is a link for The Journal of Physiology 
There are some good research articles in NMJ.
Historic researches in NMJ:
In 1672, Myasthenia gravis (MG) was first described by Thomas Willis. It is an acquired autoimmune disease with antibodies against the nicotinic acetylcholine receptor (AChR) at the NMJ. 
In1842, Claude Bernard concluded that the arrow poison curare acts at the NMJ to interrupt the stimulation of muscle by nerve impulses. 
In 1850, Claude Bernard demonstrated that the observed paralysis was mediated via the NMJ, although the precise mechanism was at that time unknown. 
In 1914, Sir Henry Dale, (in 1936, he was warded the Nobel Prize for Medicine and Physiology), investigating the pharmacological properties of ACh, distinguished two actions that were reproduced by the alkaloids, muscarine and nicotine. As the effects of muscarine mimicked the parasympathetic nervous system, he termed the receptors muscarinic, whereas those in autonomic ganglia and at the skeletal NMJ were termed nicotinic. 
In 1952 - 1954, the name end-plate potential is used since Fatt&Katz (1952) and Del Castillom & Katz (1954) studied the endplate potential (epp) at NMJ. They concluded that the ACh molecules were released in packets containing several thousand ACh molecules revealing the quantal nature of synaptic transmission. 
Abnormalities in signal transmission at the NMJ with associated diseases
This research explored that in skeletal muscle, the stability and function of NMJ depend on mitochondrial respiration. Furthermore, any abnormalities of this respiration lead to distal degeneration of motor neurons in Amyotrophic Lateral Sclerosis disease.
This is a review article for the autoimmune disorders of the neuromuscular junction.. The article unfolds the role of Acetylcholine receptors (AChR)and also the loss of AChRs at the NMJ is the underlying Myasthenia Gravis (MG)pathogenesis.However, if there is not enough ACh released at NMJ, it will lead to another disease such as The Lambert Eaton Myasthenic Syndrome.
Functional anatomy of the NMJ
All the behaviour depends on the ability to control the activity of skeletal muscles, which maintain posture and permit movement (Crossmen R.A. & Neary D., 2005). This group of muscles are innervated by axon motor neurons, the body of which lie in ventral horn of the spinal cord. The junction between the axon terminal of a motor neuron and the motor end plate of a muscle fibre make a Neuromuscular Junction (NMJ). When an action potential arrives at the axon terminal of a motor neuron, the voltage gated calcium (Ca)will open and through which Ca enters the cytosol. Then the acetylcholine is released which diffuses to and binds to cholinergic receptors at the motor end plate (Stanfield, L.C., & German, J.,W., 2009). This is followed by the opening of cation channels where the sodium enters the cell, producing an end plate potential. This cause an action potential which spread along skeletal muscle membrane stimulating contraction.
Mechanism of action
Development of the neuromuscular junction
Common neuromuscular junction disorders
Current associated research
Hey guys, I've added a sub-heading of common disorders of neuromuscular junction--Shan Ruan 10:52, 31 March 2011 (EST)
Neuromuscular Junction (NMJ)
Embryonic development of NMJ
Functional anatomy of the NMJ
The role of Acetylcholine (ACh), its receptors and inhibitors
Abnormalities in signal transmission at the NMJ with associated diseases
Current research combined with techniques used
The actual wiki page for neuromuscular junction contains the headings - --Jill De Coster 08:09, 29 March 2011 (EST)
-Mechanism of action
-Development of the neuromuscular junction
Here is a link to my old Embryology page i did last semester, we should maybe look at a similar layout? --Jill De Coster 12:20, 24 March 2011 (EST)
2. historic background
3. current associated research
4. simplified description of technique
5. student drawn figure or animation
6. reference list
8. external links
example reference list --z3265772 12:21, 24 March 2011 (EST)
This is about frog nucleoli shape changes.
- Crossmen R. A. & Neary D. (2005). Neuroanatomy: An Illustrated Colour Text. Third edition. Elsevier Churchill Livingstone 2005.
- Stanfield L.C. & German J.W.(2009). Principles of Human Physiology. Third edition. Pearson Benjamin Cummings 2009.