Difference between revisions of "Talk:2009 Group 1 Project"

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== Discussion ==
 
== Discussion ==
A. overall layout is pretty easy to navigate through. we found some of your images are not properly referenced.Perhaps you could change your subheading to make it a little clearer for the processes of meiosis, and perhaps make some of the dates in the history in bold. You could have used more external links, we couldn't find links back to your individual pages.   
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A. overall layout is pretty easy to navigate through. we found some of your images are not properly referenced and your processes of meiosis ii has NO in text references.Perhaps you could change your subheading to make it a little clearer for the processes of meiosis, and perhaps make some of the dates in the history in bold. You could have used more external links, we couldn't find links back to your individual pages.   
  
  

Revision as of 12:00, 28 May 2009

Group Project Peer Assessment

Discussion

A. overall layout is pretty easy to navigate through. we found some of your images are not properly referenced and your processes of meiosis ii has NO in text references.Perhaps you could change your subheading to make it a little clearer for the processes of meiosis, and perhaps make some of the dates in the history in bold. You could have used more external links, we couldn't find links back to your individual pages.


--Mark Hill 13:32, 24 April 2009 (EST) I can see recent contributions to this group project from Z3132868 and Z2209471 and early contributions from Z3126345 and almost nothing from Z3224430 by week 6.

--Lisa Mak 12:11, 26 March 2009 (EST)Hey everyone, shall we discuss about the subtopics of the topic Meiosis, and then separate the work over the weeks? Any suggestions?

--Lisa Mak 12:50, 26 March 2009 (EST)I'm thinking of focusing on sister chromatids for my individual project, is it okay with you all? What do you guys have in mind for yours?

--Linda Taylor 16:15, 31 March 2009 (EST)Hi all! I would like to do 'synaptonemal complex' which holds the bivalent together during prophase I and allows recombination to occur for my individual project. Hope that is OK with everyone. I am also going to post 300 or so words on Meiosis I as a start. The highlighted words are one I'll add to our glossary, when I figure that one out.

--Mark Hill 22:47, 31 March 2009 (EST) Well done Linda and Lisa, you are using the online interaction as required for the project to work well. Now where are the other members of your group?

--Gang Liu 22:14, 1 April 2009 (EST)hi, guys! so sorry, I meant to put up my notes earlier, been busy with my presentation and class quiz this week. But i'm back and ready to contribute.

--Gang Liu 23:19, 1 April 2009 (EST)Good job lisa and Linda, you guys have done some serious readings. I have no problems with topics for individual projects. For example, Lisa will be working on sister chromaids for individual project and Linda will be doing synatonemal complex for hers.

I would like to jot down some notes on meiosis process using my own words. I am using textbooks and online resources as my references. Feel free to correct me. However, it will be much easier understand via the illustration of a diagram.

  • To summerise meiosis in short, it is a process where a diploid cell duplicates its DNA once and then divides twice. As a result of this division, four new haploid cells are formed. And each of these new cells are different from parent cell.

--Gang Liu 23:22, 1 April 2009 (EST)i'm still working on glossary, but it will be posted shortly.

  • During S phase, diploid cell undergoes duplication. Two set of unique chromosomes, one from mother and one from father. Each set contains a copy of short, medium and long chromosome. As a result of duplication, each cell will have an extra copy of chromosome for short, medium and long chromosomes. And that brings a total of four short chromosome, four medium chromosome and four long chromosome.
  • In prophase, crossing over takes place. Each homologous pair of chromosomes will form tight association with each other and exchange DNA, known as crossing over. That is the tail of the chromosomes switch places. This forms a recombination of the chromosomes. After crossing over process is completed, the chromosomes dissociate from each other. And they will start to line up in the centre of a cell and ready for next phase.
  • In metaphase 1, homologous pair chromosomes line up across each other. For example, short with short, medium with medium and long with longs. In addition, the side of which pair of chromosomes lining up are not necessarily matter. As long as the pair of chromosome line up across each other.
  • During anaphase 1, the homologous pair of chromosomes are separated, and each new cell gets a homologous pair of chromosome.
  • Upon the completion of telophase, there are two cells and each of these cell has two copies of short,two copies of medium and two copies of long chromosomes. Furthermore, each of these cell is different from each other as a result of crossing over and recombination.
  • In meiosis 2, these two cells will start to divide.

--Gang Liu 13:22, 14 April 2009 (EST)--Gang Liu 13:22, 14 April 2009 (EST)

  • During metaphase of meiosis 2, chromosome will line up in a straight row, then separate. As a result of this separation, it will now have four unique cells. And each of these new cells are different from one another. Furthermore, new cells have only one copy of chromosomes, that is, one copy of short, one copy of medium and one copy of long chromosome.

--Gang Liu 23:29, 1 April 2009 (EST)well done linda on Meiosis! Guess we will have to glossary then.

--Lisa Mak 12:31, 2 April 2009 (EST)hey I found this link quite interesting in terms of meiosis animation, maybe we can add it to our project page so its more interest? hehe... "http://www.johnkyrk.com/meiosis.html" check it out=)

--Lisa Mak 12:42, 2 April 2009 (EST)http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mboc4.section.3686 link to online textbook THE CELL which has relatively good info on our topic

--Gang Liu 15:06, 13 April 2009 (EST)Hi,all! Happy easter! Would like to drop a few lines for lisa, and keep her updated regarding to last Thursday's lab and group project.

  • On thursday morning's lab, it was run by a female guest lecturer (what's her name). And we were given a two hour lecture on the topic of "use of mouse model to study the cytoskeleton". It is genetic targeting which replaces normal allele with a mutant allele. The practical aspect of the lecture was to investigate pathological dieaseas by utilising gene targeting and transgenic method.
  • For group project, keep researching. And starting writing on group main page.
  • For individual project, need to finalise individual topic and start writing on individual page.

--Lisa Mak 15:58, 13 April 2009 (EST)thank you Gary for the update!! hehe... hows everyone going with their research? are you all having a good break over easter?

--Gang Liu 13:22, 14 April 2009 (EST)Good to hear from you lisa! I had a good time during easter break, which makes me feeling guilty, not doing enough uni work all that, hehe... Will keep people updated about da project.

--Linda Taylor 15:06, 16 April 2009 (EST)Hi All, not a lot of change on our page! I've had a go at structuring our headings...feel free to edit, scrub, add or whatever. The headings of structure, function, dynamic process I don't think really work for us. I mean what is the structure of meiosis? I don't know how you would answer that one. Meiosis is a process that can be divided into phases, but all of the action takes place in Meiosis I (prophase I) and not a whole lot happens else where, so I guess that's what we should talk about. At this point in time we aint done much...I've done a couple of pars, Garry's put down some points but unless a bit more is put down on page we won't know where we are headed! Looking forward to your feedback, additions, alterations, arguments....

--Gang Liu 11:52, 18 April 2009 (EST)hi, all! I will be doing MICROTUBULE as my individual topic.

--Gang Liu 11:54, 18 April 2009 (EST)hi, linda. Thank you for your effort. And i will post my notes asap.

--Lisa Mak 16:25, 19 April 2009 (EST)Thank you Linda for your editting on the headings! Looking a lot more organized now haha, but guess we should post all our notes and get that figured out before working on the layout...Sorry I havent been doing much yet but will get to it asap!

--Jae Lee 00:14, 23 April 2009 (EST) Hi guys, it's Jae from your group. This is actually my first time putting something up in the discussion board, Meh I feel so left out! Sorry, I've been preoccupied with my two major assessments that were finished today. Anyways, I'm currently working on the project and I'll let you guys know my protein for individual project before tomorrows lab.

--Lisa Mak 20:57, 29 April 2009 (EST)hey guys so sorry, ive been typing up the drafts for my part on my word document hence havent been posting anything on our webpage yet... will get it finished by latest next week! and so sorry ive been sick for a while... caught this really bad flu and it just kept coming back...>< hope u guys are okay with ur part too! thank you linda for ur thorough presentations!

--Linda Taylor 09:43, 30 April 2009 (EST)Hi all...no worries Lisa, better thorough than nothing - we can more easily cut than fill in blanks! Hope your flu is better now. Would be great to see online contributions (drafts are good as we are only at draft stage), then we can at least see where we are headed. See you all in lab.

--Gang Liu 11:43, 30 April 2009 (EST)Hi, all! linda and i, we have discussed in lab that there are two aspects of meiosis need to be clarified at this stage: First, what is/are the biological factor(s) that initiate meiosis as opposed to mitosis; Second, what extact type of spindle is involved in meiosis (not mitotic spindle), or what are the differences between mitotic spindle and the spindles that involve in meiosis.

--Jae Lee 22:30, 1 May 2009 (EST) Hi all, I finally decided my protein for my individual project. My protein of interest is: DNA mismatch repair, which is a family of proteins that acts to repair DNA mismatches during DNA replication process in meiosis. This ensures the accuracy of replication process and thus enabling individual species to preserve their genome integrity.

--Gang Liu 17:24, 6 May 2009 (EST)Good to hear from you jay, finally everyone has their own individual topic.

  • Regarding to the discussion during last week's lab, is there any difference between meiotic spindle and mitotic spindle, as well as what are the biological factors that triger meiosis.
  • Based on my research, there is not much differece between meiotic spindle and mitotic spindle. Spindles grow out of microtubule, which make up the cytoskeleton. Spindles grow via gamma-ring strutures located on the surface of microtubule. Its function in seperating the chromosomes into the daughter cells during both meiosis and mitosis. Really, depend on what type of cell division we are referring to. In meiosis, it is referred to as meiotic spindle, In mitosis, it is referred to as mitotic spindle.
  • Exactly what triggers meiosis, research suggests unknown.

--Gang Liu 21:23, 6 May 2009 (EST)Hi, Jae, i was just wondering about your individual project topic. DNA synthesis and replication only occurs in S(synthesis) subphase during interphase, and it does not happen in Meiosis/Mitosis, so DNA mismatch repair proteins have nothing to do with Meiosis. Correct me if i am wrong.

--Lisa Mak 09:19, 7 May 2009 (EST)hey guys, sorry im still sick from the stupid stupid flu!! still fevering this morning, so didnt even go to anat lab sighhh... cant wait to get better!! anyway, ive been working on my part and will be posting them up shortly, and meanwhile, eventho im not in lab, i'll be online so if there's anything u guys need me, i'll be here. sorry that ive been away for so long...

--Linda Taylor 12:14, 7 May 2009 (EST)Jae will do regulation and simplify time-line...Gary will wok on a flow chart for meiosis and regulation

--Jae Lee 14:51, 7 May 2009 (EST) Journal article that I will use for regulation of Meiosis:

  • Stra8 and its inducer, retinoic acid, regulate meiotic initiation in both spermatogenesis and oogenesis in mice. (PMID: 18799751)
  • Regulation of the mitotic and meiotic cell cycles in the male germ line. (PMID: 12017557)
  • Function of cyclins in regulating the mitotic and meiotic cell cycles in male germ cells. (PMID: 19001847)
  • Cell cycle regulation in mammalian germ cells (PMID:16903217)
  • Cell-cycle control during meiotic maturation (PMID:14644189)
  • Regulation of the meiotic cell cycle in oocytes (PMID: 11063930)

USING THE PMID:

  • Go to the UNSW library website
  • click on the "sirius" tab
  • Click on "find Resource"
  • type in "medline"
  • A pop up should come up
  • In the new pop-up, find the search menu and click on the "find citation" tab
  • On the tab, type in PMID on the "Unique identifier" column and click on search,
  • then a search result will show the direct link to the full-text version of the Journal of your interest.


--Gang Liu 23:30, 11 May 2009 (EST)Thanks for the tip jay, it really helps!


--Gang Liu 23:33, 11 May 2009 (EST)Hi, does anyone have any idea about the word limit for individual project?

--Lisa Mak 23:29, 12 May 2009 (EST)I think its similar to the group project, that is, write anything u think relevant to your topic?

--Lisa Mak 23:42, 12 May 2009 (EST)sorry, my mistake..."The final project should not be too lengthy, as a guideline typically 500 to a maximum of 1000 words."

--Gang Liu 00:12, 13 May 2009 (EST)Thanks for the response lisa.

--Gang Liu 21:52, 13 May 2009 (EST)Hi, team! The infomation that i've posted here can also be found on the main project page.

  • Meiosis invloves the reduction of a diploid cell into four haploid chromosomes(daughter cells). Meiosis is exclusively taking place in eukaryotes. It happens in a single direction as oppose to cell cycle. Unknown mechanism initiates meiosis after interpase.
  • Meiosis flow chart:
  • -Interphase: G1 (gap 1 where cell size increases and various proteins and enzymes being syntheised)+ S phase( Synthesis phase where DNA is being replicated). Gap 2 phase does not available to meiosis.
  • -Follows by Meiosis 1 which involves the separation of homologous pair of chromosomes.
  • Meiosis 1: Formation of two haploid cells
  • Prophase1: Crossing-over of chromosomes, this result in formation of recombination of daughter cells
  • Metaphase1: Homologous chromosomes line at the equator of the meiotic spindle
  • Anaphase1: Homologous chromosomes are being pulled apart towards opposite ends of cell by meiotic spindle. Cell elongates
  • Telophase1: Marked the end of meiosis 1. Homologous chromosomes arrive at the pole. Each chromosome contains a pair of sister chromotids. New nuclear membrane forms. Cell will split apart by cytokinesis.
  • -This follows by Meiosis 2 which involves further reduction of homologous pair of chromosomes into four haploid daughter cells.
  • Meiosis2: Results in the production of four haploid cells
  • Prophase2: Sister chromotids are being condensed to facilitate the formation of spindle fibres
  • Metaphase2: Alignment of sister chromotids at the equator of metaphase plate. Formation of new metaphase plate is being rotated 90 degrees compared to metaphase 1
  • Anaphase2: The two sister chromotids are being pulled apart towards the opposite ends of cell
  • Telophase2: Similar to telophase 1, new nuclear membrane are being formed, and followed by the disappearance of spindles. As a result of cytokinesis, there are four haploid chromosomes. Meiosis ends.

--Gang Liu 00:55, 14 May 2009 (EST)meiosis flowchartMeiosis flowchart

--Linda Taylor 09:49, 14 May 2009 (EST)Hi all! I will not be at the lab today as I have a throat infection.

--Lisa Mak 11:15, 14 May 2009 (EST)Take care Linda and get well soon!

--Jae Lee 03:32, 16 May 2009 (EST) hey guys, I've done some simple restructuring and I think it looks a little better than previously. and i would just like to let you know that im still working on regulation of meiosis, and it will be completed soon~

--Jae Lee 13:51, 18 May 2009 (EST) Hey, I'm going to change my protein for the individual project to Spo11 protein, its a key protein involved in initiation of meiotic recombination. I tried to continue with my DNA mismatch repair protein... but it seemed really broad and complex. Anyways, I hope you guys are progressing well with your individual assignment-!

--Linda Taylor 15:06, 26 May 2009 (EST)Hi guys, have just done a bit of editing. Jae, thanks for the Regulation posts and Lisa, thanks for Meiosis II and Aneuploidy. We still need some graphics and the hand drawn thingy! Why don't we each post one picture and Gary can do the hand drawing?

--Jae Lee 02:49, 27 May 2009 (EST) hey Linda~ thanks you for your editing and especially the history part...! I was just wondering if u can put all your references in the "referneces" heading at the bottom of the page, and I think you can do that by simply removing "<referennces/>" code. Secondly, i was thinking about adding another section: "meiosis in male and female" which will show similarity and difference between male and female meiosis, which hopefully will provide bit more depth to the whole topic... what do you think guys?

--Lisa Mak 17:44, 27 May 2009 (EST)yea go ahead Jae~ sounds good =) I was wondering about the Group Reflection 500 words... what is that about?

--Linda Taylor 20:19, 27 May 2009 (EST)Hi guys, I've added a bit on The differences Between Male and Female Meiosis. Feel free, as always, to add, edit...whatever. I've also added a pic. Regarding references, I personally think references should be within a section otherwise reference list will become overwhelming, and well, a bit meaningless. Just my view... But if you feel strongly about it being all at end - feel free to edit, add...whatever. I am happy with consistancy. See you all in Lab.

--Gang Liu 21:08, 27 May 2009 (EST)hi all, We will need to devote a section on "Group Reflection". In this section, we will discuss all the editings throughout the session, describe any changes made by peers to group wiki page and reflect on these changes(eg. a particular editing or change, what do we learn from it). To sum up, it is a log of our wiki page. We will mention everthing we have done so far, and what do we get out from all the editings and changes. If you guys are ok with it, i would like to work on this section.

--Gang Liu 21:22, 27 May 2009 (EST)sorry i made a mistake. It is asking us to reflect on changes and editings from week 3 to week 10.

--Gang Liu 21:34, 27 May 2009 (EST)hi, one more thing. In the Course manual, it mentions "Download(eg. by copying and pasting) all of the edits made in the exposed wiki in the edit time period. Attach this to your report as supplementary material". Does this mean we can attach list under history section? I'm a bit confused.

--Jae Lee 21:52, 27 May 2009 (EST) Meh- i don't get it either, but hopefully Dr Hill will clarify it on tomorrow's lab.... anyways Linda, thank you for your contribution on male/Female meiosis part, especially the mechanism underlying it. And... thank you Lisa for doing the current research part, our lecturer mentioned in today's lecture that this part will be part of the marking criteria. Finally just some comments on the images that has been uploaded on to the page... can you put in the source of the image and its copyright details? --Gang Liu 10:48, 28 May 2009 (EST) hi all, at last, i've managed to put details in group reflection section, hopefully this matches to the marking criteria.