Group 3 Project- Immunohistochemistry

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What is Immunohistochemistry?

Immunohistochemistry (IHC) is a technique used to locate antigens or proteins in tissue sections. This is possible by using labelled antibodies which react with specific antigens in an antibody-antigen interaction. This interaction is visualised by a marker. Markers may be fluorescent dyes, enzymes, radioactive elements or colloidal gold.

History

Methods

Direct Method

Indirect Method

PAP Method

ABC Method

The ABC method in immunohistochemistry is a type of indirect method and stands for Avidin-Biotin Complex method.


LSAB Method

2010 Projects

Fluorescent-PCR | RNA Interference | Immunohistochemistry | Cell Culture | Electron Microsopy | Confocal Microscopy | Monoclonal Antibodies | Microarray | Fluorescent Proteins | Somatic Cell Nuclear Transfer