Difference between revisions of "Group 3 Project- Immunohistochemistry"

From CellBiology
Line 1: Line 1:
 
==What is Immunohistochemistry?==
 
==What is Immunohistochemistry?==
  
 
+
Immunohistochemistry (IHC) is a technique used to locate antigens or proteins in tissue sections. This is possible by using labelled antibodies which react with specific antigens in an antibody-antigen interaction. This interaction is visualised by a marker. Markers may be fluorescent dyes, enzymes, radioactive elements or colloidal gold.
 
 
  
 
==History==
 
==History==

Revision as of 17:12, 11 April 2010

What is Immunohistochemistry?

Immunohistochemistry (IHC) is a technique used to locate antigens or proteins in tissue sections. This is possible by using labelled antibodies which react with specific antigens in an antibody-antigen interaction. This interaction is visualised by a marker. Markers may be fluorescent dyes, enzymes, radioactive elements or colloidal gold.

History

Methods

Direct Method

Indirect Method

PAP Method

2010 Projects

Fluorescent-PCR | RNA Interference | Immunohistochemistry | Cell Culture | Electron Microsopy | Confocal Microscopy | Monoclonal Antibodies | Microarray | Fluorescent Proteins | Somatic Cell Nuclear Transfer