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(Colocalization of SlrP and ERdj3 in HeLa cells. A, microscopic analysis. HeLa cells transiently transfected with plasmids expressing SlrP-3×FLAG and ERdj3–3×HA were observed in a laser-scanning confocal microscope after permeabilization and staining w)
 
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Latest revision as of 22:56, 18 May 2010

Colocalization of SlrP and ERdj3 in HeLa cells. A, microscopic analysis. HeLa cells transiently transfected with plasmids expressing SlrP-3×FLAG and ERdj3–3×HA were observed in a laser-scanning confocal microscope after permeabilization and staining with Alexa Fluor® 488 labeled anti-HA (green, upper panel) and Cy3-conjugated anti-FLAG (red, center panel). Superposition of the two labelings is shown in the lower panel. Yellowish staining indicates colocalization of SlrP and ERdj3 in the endoplasmic reticulum. Scale bar, 10 μm.

"This research was originally published in Journal of Biological Chemistry. Bernal-Bayard J, Cardenal-Muñoz E, Ramos-Morales F. The Salmonella type III secretion effector, salmonella leucine-rich repeat protein (SlrP), targets the human chaperone ERdj3. J Bio Chem. 2010 May 21;285(21):16360-8. Epub 2010 Mar 24. © the American Society for Biochemistry and Molecular Biology."

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current22:56, 18 May 2010Thumbnail for version as of 22:56, 18 May 2010642 × 672 (68 KB)Z3253199 (talk | contribs)Colocalization of SlrP and ERdj3 in HeLa cells. A, microscopic analysis. HeLa cells transiently transfected with plasmids expressing SlrP-3×FLAG and ERdj3–3×HA were observed in a laser-scanning confocal microscope after permeabilization and staining w

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