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Macrophage pseudopod extension occurs in two phases during FcγR-mediated frustrated phagocytosis and correlates with membrane and cytoskeleton dynamics.

(A) Spinning-disk confocal microscopy of a macrophage undergoing frustrated phagocytosis showing plasma membrane (PM-GFP, Upper) and F-actin (Ruby-Lifeact, Lower). A single confocal section at the glass interface is shown. Numbers indicate time in seconds (Movie S1). (Scale bar, 10 µm.) (B) Motility map of the cell in A showing protrusion velocity along the circumferential cell edge as a function of time. P1 and P2 indicate the motility phases before and following the transition, T, respectively. (C) Cell-contact area at the transition versus initial cell size on first contact with the opsonized surface. (D) Zoom from plasma membrane in A. Folds of plasma membrane (bright puncta visible in the first image) are exhausted before the transition. (E) Three-dimensional reconstruction of a spinning-disk microscopy time series during frustrated phagocytosis. Dorsal membrane folds (clearly visible at 0 s) are exhausted before the transition (at 120 s). Subsequently, ruffles and folds (indicated by the white arrow) recover on the cell surface.

Reference

<pubmed>23821745</pubmed> | Pubmed

Copyright

@2013 Masters et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: ‘Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis' Thomas A. Masters, Bruno Pontes, Virgile Viasnoffa, You Li, and Nils . Gauthier, (2013) Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11875-80. doi: 10.1073/pnas.1301766110. Epub 2013 Jul 2.

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current13:35, 3 April 2014Thumbnail for version as of 13:35, 3 April 2014768 × 288 (92 KB)Z3375490 (talk | contribs)Macrophage pseudopod extension occurs in two phases during FcγR-mediated frustrated phagocytosis and correlates with membrane and cytoskeleton dynamics. (A) Spinning-disk confocal microscopy of a macrophage undergoing frustrated phagocytosis showing p...

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