File:Plant organelles tem1.jpg
Plant Organelles tem
Fig. 2. Transmission electron micrographs showing a comparison of glutathione labelling density between mesophyll cells from leaves of the wild type and different mutants of Arabidopsis plants. Different amounts of gold particles can be observed between the Arabidopsis wild-type Col-0 (A), the GSH1 overexpressing line OE2 (B), and the pad2-1 mutant (C, D). Note that cells of the GSH1 overexpressing line (B) show, in general, higher amounts of gold particles bound to glutathione when compared to Col-0. By contrast, cells of the pad2-1 mutant (C, D) show fewer gold particles bound to glutathione, except in mitochondria where gold particle density was similar to Col-0 (A). Pre-adsorption of the anti-glutathione antibody with an excess of free glutathione prior to its application (for details see Materials and methods) resulted in the complete absence of gold particles bound to glutathione in all cell compartments of the pad2-1 mutant (E). C, chloroplasts; St, starch; CW, cell walls; IS, intercellular spaces; M, mitochondria; N, nuclei; Px, peroxisomes; V, vacuoles. Sections were post-stained with uranyl acetate for 15 s. Bars: 1 μm.
Subcellular immunocytochemical analysis detects the highest concentrations of glutathione in mitochondria and not in plastids. Zechmann B, Mauch F, Sticher L, Müller M. J Exp Bot. 2008;59(14):4017-27. PMID: 18977750
J Exp Bot. 2008 October; 59(14): 4017–4027. doi: 10.1093/jxb/ern243. Copyright © 2008 The Author(s).
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