File:Phosphorylation of Lamin B1.jpg

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Phosphorylation of Lamin B1 by CDK1 and PKC

Original file name: JCB_201205103_Fig1.jpg

This image highlights the importance of Lamin B1 phosphorylation, by CDK1 and PKC, in the disassembly of the nuclear lamina. (A) A schematic diagram of the structure and amino acid sequence of Lamin B1 showing the various phosphorylation sites along the polypeptide(yellow streaks and asterisks)which have CDK1 (blue) and PKC (maroon) binding sites

(B) Shows mutations at CDK1 and/or PKC phosphorylation sites on the Lamin B1 polypeptide

(C) These are Confocal time-lapse microscopy images of HeLa cells going through the different stages of mitosis and expressing lamin B1 receptor which has been fluorescently tagged (grey). The effect of mutations in the phosphorylation sites of lamin B1 is shown through the comparison of the mutants with the wild type and the arrow-heads which indicate remaining lamin B1 fragments present after prometaphase. --Z3331556 (talk) 13:35, 28 May 2013 (EST)

Original legend: Phosphorylation sites on lamin B1 promote mitotic disassembly. (A) Schematic representation of lamin B1 domain structure and phosphorylation (UniProt). The central rod is flanked by several mitotic phosphorylation sites (indicated by asterisks) that exhibit CDK1 and PKC consensus motifs (Pearson and Kemp, 1991). (B) Overview of lamin B1 reporters containing serine (S) to alanine (A) mutations at CDK1 and/or PKC consensus phosphorylation sites. (C) Confocal time-lapse microscopy images of HeLa cells stably expressing H2B-mCherry and transiently expressing the indicated lamin B1 reporter tagged with GFP (gray). Progression through mitosis was followed, and time relative to chromatin condensation in prophase is shown. Arrowheads indicate cytoplasmic lamin B1 fragments present after prometaphase. Bars, 10 µm.

Reference

<pubmed>PMC3444782</pubmed>

Mitotic lamin disassembly is triggered by lipid-mediated signaling

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444782/#!po=13.1579

Copyright

Copyright © 2012 Mall et al.

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current12:11, 28 May 2013Thumbnail for version as of 12:11, 28 May 2013672 × 719 (207 KB)Z3331556 (talk | contribs)Original file name: JCB_201205103_Fig1.jpg Mitotic lamin disassembly is triggered by lipid-mediated signaling http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444782/#!po=13.1579 Phosphorylation sites on lamin B1 promote mitotic disassembly. (A) Schemat...
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