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Nuclear Envelope Remnants: Fluid Membranes Enriched in STEROLS and Polyphosphoinositides
FIGURE 1: Nuclear envelope remnants contain two membranes that line the acrosomal and centriolar fossae. (A) P. lividus sperm cells were fixed in the presence of 1% (w/v) tannic acid. The plasma membrane (PM) and the mitochondrial membranes (MM) are shown. AV: acrosomal vesicle, N: nucleus, F: flagellum. The nuclear envelope is tightly apposed to the chromatin but cup-like structures with nuclear envelope remnants can be seen at the poles (arrows). (B and C) S. purpuratus 0.1% nuclei were incubated in egg cytoplasm supplemented with ATP-GS and fixed in the presence of 1% (w/v) tannic acid. Electron dense structures (arrows) are shown in the centriolar (B) and acrosomal fossae (C). The two bilayers appear to have variable amounts of electron dense material between them. (D) Cryosections of S. purpuratus 0.1% nuclei prefixed in 4% (v/v) formaldehyde for 3h on ice show two membranes in the centriolar fossa (arrows). (E) S. purpuratus 0.1% nuclei were incubated in egg cytoplasm in the presence of an ATP-generating system, fixed in 2.5% (v/v) glutaraldehyde in the presence of 1% (w/v) tannic acid and viewed by TEM. The glancing cross section of the centriolar fossa shows the nuclear envelope remnants and an egg membrane vesicle (arrow) associated with the nuclear envelope remnants. Bars are 500nm (A), 400nm (B) and 200nm (C, D and E). The data are representative of nuclei observed in at least 3 experiments on independent nuclei preparations. doi:10.1371/journal.pone.0004255.g001
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|current||18:00, 20 March 2014||419 × 600 (1.12 MB)||Z3399239||Nuclear_membrane.png http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004255 Nuclear Envelope Remnants: Fluid Membranes Enriched in STEROLS and Polyphosphoinositides FIGURE 1: Nuclear envelope remnants contain two membranes that line th...|