File:Nuclear Proteins in ER.jpg
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Original file name: JCB.14511f3.jpg
Integral Membrane Proteins of the Nuclear Envelope Are Dispersed throughout the Endoplasmic Reticulum during Mitosis
Comparison of the localization of ER membranes to the distributions of LAP2, gp210, and α-mannosidase II in metaphase cells. NRK cultures enriched in mitotic cells were fixed and examined by double labeling with DiOC6 and the LAP2-specific monoclonal antibody RL29 (A), DiOC6 and the gp210-specific monoclonal antibody RL20 (B), or R6 and a polyclonal antibody against the Golgi enzyme α-mannosidase II (C). Shown is the labeling with the ER probes (left column), antibody probes (center column), and a merge of the two fluorescent images (right column). The green/red color representation for the antibody and R6 was computationally reversed in the images presented in C for consistency. In A and B, two different metaphase cells are shown, and in C, a metaphase (left cell) and anaphase (right cell) are shown. Bar, 10 μm.
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|current||22:30, 27 May 2013||640 × 520 (195 KB)||Z3331556||Original file name: JCB.14511f3.jpg Integral Membrane Proteins of the Nuclear Envelope Are Dispersed throughout the Endoplasmic Reticulum during Mitosis http://www.ncbi.nlm.nih.gov.wwwproxy0.library.unsw.edu.au/pmc/articles/PMC2132536/ Comparison of...|