File:IF desmin mutants.jpg
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Overview of the assembly and decay pathway of various desmin mutants
a | When soluble tetrameric complexes of wild-type (WT) desmin are induced to assemble, initially eight tetramers associate laterally to form a unit-length filament (ULF). Next, these ULFs anneal longitudinally and give rise to short, 'open' (that is, less compact) filaments. Last, elongated filaments radially compact to yield mature intermediate filaments (IFs). An example of mature desmin IFs is depicted in the electron microscopy image of negatively stained preparations of in vitro assembled mouse recombinant desmin.
b | Mutant desmins assemble into various types of structures that can be classified into four types, exemplified here by: DesA360P, forming IFs of seemingly normal appearance (panel 1); DesR406W, being arrested during elongation and thereby exhibiting short, still segmented filaments (panel 2); DesN342D, in addition to extensive elongation, individual filaments have opened along their length and generated meshworks of protofilamentous masses (panel 3); DesL370P, initially successfully assembles into ULF-like structures and short regular filaments, but within 20 seconds reorganizes into relatively regular, round aggregates that are 30 nm in diameter (panel 4).
All micrographs represent negatively stained specimens prepared and recorded under identical conditions.
For more details, see Ref. 79. Scale bars, 100 nm.
FROM THE FOLLOWING ARTICLE: Intermediate filaments: from cell architecture to nanomechanics Harald Herrmann, Harald Bär, Laurent Kreplak, Sergei V. Strelkov & Ueli Aebi Nature Reviews Molecular Cell Biology 8, 562-573 (July 2007) doi:10.1038/nrm2197
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|current||14:56, 7 April 2009||655 × 417 (87 KB)||S8600021||FIGURE 4 | Overview of the assembly and decay pathway of various desmin mutants. a | When soluble tetrameric complexes of wild-type (WT) desmin are induced to assemble, initially eight tetramers associate laterally to form a unit-length filament (ULF). N|