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==Polymerase Chain Results==
 
==Polymerase Chain Results==
 
This image shows part of the results gained from an experiment testing the affect of estrogen on the expression of cartilage-specific genes in the chondrogenesis process of adipose-derived stem cells<ref name=”PMID25789269”><pubmed> 25789269</pubmed></ref>.  
 
This image shows part of the results gained from an experiment testing the affect of estrogen on the expression of cartilage-specific genes in the chondrogenesis process of adipose-derived stem cells<ref name=”PMID25789269”><pubmed> 25789269</pubmed></ref>.  
est-: estrogen negative group
+
 
est+: estrogen postive group
+
'''est-:''' estrogen negative group
AGR: aggrecan group
+
 
COL: collagen II experimental group
+
'''est+:''' estrogen postive group
GAPDH: Glyceraldehyde 3-phosphate dehydrogenase group
+
 
 +
'''AGR:''' aggrecan group
 +
 
 +
'''COL:''' collagen II experimental group
 +
 
 +
'''GAPDH:''' Glyceraldehyde 3-phosphate dehydrogenase group
 +
 
 
Results showed the aggrecan gene expression in the experimental group (with estrogen) was significantly lower than control (without estrogen) and collagen II was not expressed in experimental group. GAPDH was considered as house-keeping gene.
 
Results showed the aggrecan gene expression in the experimental group (with estrogen) was significantly lower than control (without estrogen) and collagen II was not expressed in experimental group. GAPDH was considered as house-keeping gene.
 
Type II collagen expressed in the control group, but no band was observed in the experimental group. The expression of aggrecan was detected in both experimental and control groups. This shows that there was a statistically significant result with the difference in aggrecan expression between the estrogen positive and estrogen negative groups. Estrogen was seen to have a negative effect on aggrecan expression.
 
Type II collagen expressed in the control group, but no band was observed in the experimental group. The expression of aggrecan was detected in both experimental and control groups. This shows that there was a statistically significant result with the difference in aggrecan expression between the estrogen positive and estrogen negative groups. Estrogen was seen to have a negative effect on aggrecan expression.
 
  
 
==Copyright==
 
==Copyright==

Latest revision as of 11:18, 3 June 2015

Polymerase Chain Results

This image shows part of the results gained from an experiment testing the affect of estrogen on the expression of cartilage-specific genes in the chondrogenesis process of adipose-derived stem cells[1].

est-: estrogen negative group

est+: estrogen postive group

AGR: aggrecan group

COL: collagen II experimental group

GAPDH: Glyceraldehyde 3-phosphate dehydrogenase group

Results showed the aggrecan gene expression in the experimental group (with estrogen) was significantly lower than control (without estrogen) and collagen II was not expressed in experimental group. GAPDH was considered as house-keeping gene. Type II collagen expressed in the control group, but no band was observed in the experimental group. The expression of aggrecan was detected in both experimental and control groups. This shows that there was a statistically significant result with the difference in aggrecan expression between the estrogen positive and estrogen negative groups. Estrogen was seen to have a negative effect on aggrecan expression.

Copyright

Copyright : © 2015 Sadeghi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Reference

  1. <pubmed> 25789269</pubmed>
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current11:16, 3 June 2015Thumbnail for version as of 11:16, 3 June 2015741 × 531 (32 KB)Z3333429 (talk | contribs)==Polymerase Chain Results This image shows part of the results gained from an experiment testing the affect of estrogen on the expression of cartilage-specific genes in the chondrogenesis process of adipose-derived stem cells<ref name=”PMID25789269...

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