Endocytosis Movie 1
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Movie shows the endocytic transport intermediates delivering STxB, CTxB, and GPI-GFP to the Golgi complex.
Selective photobleaching and time-lapse imaging to visualize delivery of GPI-GFP to the Golgi complex. A series of images of a small region of the cell adjacent to the Golgi complex were rapidly collected immediately after photobleaching of the GPI-GFP fluorescence associated with the Golgi complex and the surrounding area (Video 4, A–D). Small transport intermediates containing GPI-GFP were observed tracking into the photobleached Golgi region where they disappeared, presumably due to fusion with the Golgi complex and subsequent dispersal of fluorescence.
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Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G