Endocytosis Movie 1

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<mediaplayer width='480' height='360' image="http://cellbiology.med.unsw.edu.au/cellbiology/images/7/7b/Endocytosis_1-icon.jpg">File:Endocytosis 1.mp4</mediaplayer>
Endocytosis 1-icon.jpg


Movie shows the endocytic transport intermediates delivering STxB, CTxB, and GPI-GFP to the Golgi complex.

Selective photobleaching and time-lapse imaging to visualize delivery of GPI-GFP to the Golgi complex. A series of images of a small region of the cell adjacent to the Golgi complex were rapidly collected immediately after photobleaching of the GPI-GFP fluorescence associated with the Golgi complex and the surrounding area (Video 4, A–D). Small transport intermediates containing GPI-GFP were observed tracking into the photobleached Golgi region where they disappeared, presumably due to fusion with the Golgi complex and subsequent dispersal of fluorescence.

Links: 1.5 Mb MP4 version | 4 Mb MP4 version | Movies


<pubmed>11331304</pubmed>| JCB

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Video 4A http://jcb.rupress.org/content/suppl/2001/04/30/153.3.529.F5.DC1

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G