Difference between revisions of "Endocytosis Movie 1"

Revision as of 13:37, 26 March 2013

<mediaplayer width='480' height='360' image="http://cellbiology.med.unsw.edu.au/cellbiology/images/7/7b/Endocytosis_1-icon.jpg">File:Endocytosis 1.mp4</mediaplayer>

Endocytosis

Movie shows the endocytic transport intermediates delivering STxB, CTxB, and GPI-GFP to the Golgi complex.

Selective photobleaching and time-lapse imaging to visualize delivery of GPI-GFP to the Golgi complex. A series of images of a small region of the cell adjacent to the Golgi complex were rapidly collected immediately after photobleaching of the GPI-GFP fluorescence associated with the Golgi complex and the surrounding area (Video 4, A–D). Small transport intermediates containing GPI-GFP were observed tracking into the photobleached Golgi region where they disappeared, presumably due to fusion with the Golgi complex and subsequent dispersal of fluorescence.

Links: 1.5 Mb MP4 version | 4 Mb MP4 version | Movies

Reference

<pubmed>11331304</pubmed>| JCB

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Video 4A http://jcb.rupress.org/content/suppl/2001/04/30/153.3.529.F5.DC1

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G

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 | width=500px|<mediaplayer width='480' height='360' image="http://cellbiology.med.unsw.edu.au/cellbiology/images/7/7b/Endocytosis_1-icon.jpg">File:Endocytosis 1.mp4</mediaplayer>
 | [[File:Endocytosis_1-icon.jpg|200px|right]]
-===View of many Carriers===
+===Endocytosis===
+Movie shows the endocytic transport intermediates delivering STxB, CTxB, and GPI-GFP to the Golgi complex.
+Selective photobleaching and time-lapse imaging to visualize delivery of GPI-GFP to the Golgi complex. A series of images of a small region of the cell adjacent to the Golgi complex were rapidly collected immediately after photobleaching of the GPI-GFP fluorescence associated with the Golgi complex and the surrounding area (Video 4, A–D). Small transport intermediates containing GPI-GFP were observed tracking into the photobleached Golgi region where they disappeared, presumably due to fusion with the Golgi complex and subsequent dispersal of fluorescence.
 :'''Links:''' [[Media:Endocytosis 1.mp4|1.5 Mb MP4 version]] |  [[Media:Endocytosis 1 large.mp4|4 Mb MP4 version]] | [[Movies]]