Cell Import - Endocytosis

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Exocytosis and Endocytosis cartoon

Cell Import - Endocytosis

Endocytosis types
Endocytosis

This lecture on endocytosis will be presented by Dr Till Boecking (Publications).


Associate Professor Till Böcking leads the Molecular Machines Group in the EMBL Australia Node for Single Molecule Science.


His slides will be added to this page when available.


The content below is for information purposes only and contains links to the online textbooks. This will not be used for the lecture presentation.

We will study this topic at the level of the cellular components and organelles involved in the process: endosomes, lysosomes, peroxisomes, transport vesicles, Golgi apparatus and endoplasmic reticulum.

Objectives

  • Understanding of the process of endocytosis
  • Understanding structure and function of organelles and structures associated with substance import (endocytosis) including:
    • endosomes, lysosomes, peroxisomes, Golgi apparatus and endoplasmic reticulum
  • Understanding of other cell import mechanisms, phagocytosis etc.
  • Brief understanding of signal, viral and bacterial entry into the cell
  • Brief understanding of transport within the cell

Archive: 2016 | 2014 | 2013 | 2012 | 2011 Print Version | 2010 | 2009 | 2008 | 2006


Microtubule movie 2.jpg
 ‎‎Microtubules
Page | Play
Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells. Barbero P, Bittova L, Pfeffer SR. J Cell Biol. 2002 Feb 4;156(3):511-8. Epub 2002 Feb 4. PMID 11827983 GFP-Rab9-bearing organelles and vesicles display bidirectional motility along microtubules. Speed is 5 times actual.

References

Textbooks

Essential Cell Biology

  • Chapter 14 Intracellular Compartments and Transport, Endocytic Pathways p472

Molecular Biology of the Cell

Alberts, Bruce; Johnson, Alexander; Lewis, Julian; Raff, Martin; Roberts, Keith; Walter, Peter New York and London: Garland Science; c2002

Molecular Cell Biology

Lodish, Harvey; Berk, Arnold; Zipursky, S. Lawrence; Matsudaira, Paul; Baltimore, David; Darnell, James E. New York: W. H. Freeman & Co.; c1999

The Cell- A Molecular Approach

Cooper, Geoffrey M. Sunderland (MA): Sinauer Associates, Inc.; c2000

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Books

PubMed

  • PubMed is a service of the U.S. National Library of Medicine that includes over 18 million citations from MEDLINE and other life science journals for biomedical articles back to 1948. PubMed includes links to full text articles and other related resources. PubMed
  • PubMed Central (PMC) is a free digital archive of biomedical and life sciences journal literature at the U.S. National Institutes of Health (NIH) in the National Library of Medicine (NLM) allowing all users free access to the material in PubMed Central. PMC
  • Online Mendelian Inheritance in Man (OMIM) is a comprehensive compendium of human genes and genetic phenotypes. The full-text, referenced overviews in OMIM contain information on all known mendelian disorders and over 12,000 genes. OMIM
  • Entrez is the integrated, text-based search and retrieval system used at NCBI for the major databases, including PubMed, Nucleotide and Protein Sequences, Protein Structures, Complete Genomes, Taxonomy, and others Entrez

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Reviews

  • The endocytic matrix. Scita G, Di Fiore PP. Nature. 2010 Jan 28;463(7280):464-73. Review. PMID: 20110990
  • Endocytosis and signalling: intertwining molecular networks. Sorkin A, von Zastrow M. Nat Rev Mol Cell Biol. 2009 Sep;10(9):609-22. Review. PMID: 19696798
  • PMID: 15030567

Articles

Images


Movies

  • Endosome to trans-Golgi traffic is vesicular Coexpression of fluorescent Rab9 and Rab7 by Barbero et al. revealed that these two late endosome Rabs occupy distinct domains within late endosome membranes. Rab9 is present on endosomes that display bidirectional microtubule-dependent motility, and Rab9-positive transport vesicles (rather than tubules) can be seen moving along microtubule tracks and fusing with the trans-Golgi network.
  • Recycling of endosomes by Arf6 Activation of nucleotide exchange on Arf6 causes an increase in both membrane internalization and the return of the resultant structures to the plasma membrane. However, when Brown et al. block Arf6 in its GTP-loaded ("on") state, this results in accumulation of endosome-derived vacuoles.
  • Actin tails form behind motile endosomes and lysosomes Taunton et al. document the formation of actin comet tails (red) behind motile endosomes and lysosomes. The tails may help the organelles move around the cell, and influence the construction of the cytoskeleton.
  • Lysosomes repair the plasma membrane When cells are injured, calcium rushes in and prompts exocytosis of membranes to repair the gap. Jaiswal et al. confirm that the repairing membranes are from lysosomes.

2017 Course Content

Moodle

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2017 Revision

2017 Laboratories: Introduction to Lab | Fixation and Staining |


2017 Projects: Group 1 - Delta | Group 2 - Duct | Group 3 - Beta | Group 4 - Alpha

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G