Difference between revisions of "Cell Biology Method - Gene Expression"

From CellBiology
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==Adenovirus==
 
==Adenovirus==
 
* The AdEasy system is used to rapidly generate recombinant adenovirus without the need for time consuming plaque purification.  
 
* The AdEasy system is used to rapidly generate recombinant adenovirus without the need for time consuming plaque purification.  
* Developed by T.C He et al, the AdEasy system exploits E. coli's robust, efficient recombination machinery thereby avoiding restriction-ligation involving the unwieldy (36kb) adenovirus genome.  
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* Developed by T.C He et al.<ref><pubmed>9482916</pubmed></ref>, the AdEasy system exploits E. coli's robust, efficient recombination machinery thereby avoiding restriction-ligation involving the unwieldy (36kb) adenovirus genome.  
 
* Bacterial and 293 cells that are essential to the technique to generate recombinant adenoviruses.  
 
* Bacterial and 293 cells that are essential to the technique to generate recombinant adenoviruses.  
 
* A highly detailed user manual is available for beginners as well as those experienced in this field of work.
 
* A highly detailed user manual is available for beginners as well as those experienced in this field of work.
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{{Methods Links}}
 
{{Methods Links}}
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==References==
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<references />
  
 
{{Footer}}
 
{{Footer}}

Revision as of 10:57, 31 August 2011

Adenovirus

  • The AdEasy system is used to rapidly generate recombinant adenovirus without the need for time consuming plaque purification.
  • Developed by T.C He et al.[1], the AdEasy system exploits E. coli's robust, efficient recombination machinery thereby avoiding restriction-ligation involving the unwieldy (36kb) adenovirus genome.
  • Bacterial and 293 cells that are essential to the technique to generate recombinant adenoviruses.
  • A highly detailed user manual is available for beginners as well as those experienced in this field of work.


  • OGTR certification (Cert-1830) PC2 Laboratory G29 from 11/02/2009 until 30/03/2014.
Links: Ad-Easy | 1998 PNAS paper PDFqbiogene


Methods Links: Tissue Culture | Immunochemistry | Gene Expression

References

  1. <pubmed>9482916</pubmed>

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G