2016 Lab 4 - CRISPR/Cas9
For Lab 4 class working in your Project Group, design an experiment employing CRISPR knockout technologies that would investigate a human disease.
The experiment should have:
1. Hypothesis (the hypothesis you are testing)
2. Aims (a series of specific aims of your experiment)
3. Method (the design of your KO experimental procedure, referenced)
4. Results (how the results could be interpreted/tested)
Search: NCBI databases - CRISPR | PubMed CRISPR | PubMed Centrap CRISPR
See also video JoVE Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Please paste your experiment under the appropriate group sub-heading below.
Contents
Group 1
Group 2
Hypothesis: Knocking out the SEC23B gene in mice will result in the development of an anaemia similar to Congenital Dyserythropoietic Anaemia Type 2 in humans
Aims:
Methods:
Results:
Group 3
Group 4
Group 5
1. Hypothesis (the hypothesis you are testing)
That correcting the genetic mutation in sickle cell anemia can cure the disease in mice
2. Aims (a series of specific aims of your experiment)
To use CRISPR to investigate whether genetically correcting the Sickle cell anemia can cure the disease when Rats develop in life.
3. Method (the design of your KO experimental procedure, referenced) Sickle cell disease involves a single point mutation in the seventh codon in the β-globin gene. Mice embryos identified with these single point mutations will undergo CRISPR methods to edit and correct this point mutation.
<pubmed> 25733580 </pubmed>
4. Results (how the results could be interpreted/tested)
Group 6
Hypothesis (the hypothesis you are testing)
Aims (a series of specific aims of your experimental design)
Method (the design of your KO experimental procedure, referenced)
Results (how the results/outcomes could be interpreted/tested)
Three groups Control (PrPC) protein and prion form of alpha synclein disease causing agent KO (PrPC) protein and prion form of alpha synclein disease causing agent
Group 7
2016 Course Content
Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2016 Revision
Laboratories: Introduction to Lab | Microscopy Methods | Preparation/Fixation | Cell Knockout Methods | Cytoskeleton Exercise | Immunochemistry | Project Work | Confocal Microscopy | Tissue Culture | Stem Cells Lab | Microarray Visit
Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G
