Difference between revisions of "2016 Lab 4 - CRISPR/Cas9"

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3. Method (the design of your KO experimental procedure, referenced)
 
3. Method (the design of your KO experimental procedure, referenced)
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Sickle cell disease involves a single point mutation in the seventh codon in the  β-globin gene. Mice embryos identified with these single point mutations will undergo CRISPR methods to edit and correct this point mutation.  
 
Sickle cell disease involves a single point mutation in the seventh codon in the  β-globin gene. Mice embryos identified with these single point mutations will undergo CRISPR methods to edit and correct this point mutation.  
  

Revision as of 13:15, 7 April 2016

DNA targeting platforms for genome editing

For Lab 4 class working in your Project Group, design an experiment employing CRISPR knockout technologies that would investigate a human disease.

The experiment should have:

1. Hypothesis (the hypothesis you are testing)

2. Aims (a series of specific aims of your experiment)

3. Method (the design of your KO experimental procedure, referenced)

4. Results (how the results could be interpreted/tested)


Search: NCBI databases - CRISPR | PubMed CRISPR | PubMed Centrap CRISPR

See also video JoVE Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9


Please paste your experiment under the appropriate group sub-heading below.

Group 1

Group 2

Hypothesis: Knocking out the SEC23B gene in mice will result in the development of an anaemia similar to Congenital Dyserythropoietic Anaemia Type 2 in humans

Aims:

Methods:

Results:


Group 3

Group 4

Group 5

1. Hypothesis (the hypothesis you are testing)

That correcting the genetic mutation in sickle cell anemia can cure the disease in mice

2. Aims (a series of specific aims of your experiment)

To use CRISPR to investigate whether genetically correcting the Sickle cell anemia can cure the disease when Rats develop in life.


3. Method (the design of your KO experimental procedure, referenced)

Sickle cell disease involves a single point mutation in the seventh codon in the β-globin gene. Mice embryos identified with these single point mutations will undergo CRISPR methods to edit and correct this point mutation.

<pubmed> 25733580 </pubmed>

4. Results (how the results could be interpreted/tested)



Group 6

Hypothesis (the hypothesis you are testing)

Aims (a series of specific aims of your experimental design)

Method (the design of your KO experimental procedure, referenced)

Results (how the results/outcomes could be interpreted/tested)

Three groups Control (PrPC) protein and prion form of alpha synclein disease causing agent KO (PrPC) protein and prion form of alpha synclein disease causing agent



Group 7



2016 Course Content

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2016 Revision


Laboratories: Introduction to Lab | Microscopy Methods | Preparation/Fixation | Cell Knockout Methods | Cytoskeleton Exercise | Immunochemistry | Project Work | Confocal Microscopy | Tissue Culture | Stem Cells Lab | Microarray Visit


2016 Projects: Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G