Difference between revisions of "2010 Lecture 23"

From CellBiology
Line 21: Line 21:
[[Media:Development lecture 1 per page.pdf|Development Lecture 2010 1image/page(PDF)]]
[[Media:Development lecture 1 per page.pdf|Development Lecture 2010 1image/page(PDF)]]
==Lecture Summary==
* Messengers and Receptors
* chemical signals
* cellular receptors
* signal transduction
* intracellular pathways
* second  messengers
* Examples of signaling
==Honours Projects - Dr Steve Palmer==
==Honours Projects - Dr Steve Palmer==

Revision as of 12:57, 25 May 2010


This lecture is about: – how the embryo makes use of cellular mechanisms (described during this term) to construct itself. It is concerned with concepts rather than detail. You should appreciate how these cellular mechanisms integrate to produce the whole organism.

CSt1 zygote.gif Human CST23.gif

The image above shows the first cell that forms following fertilization, and that cell's offspring 8 weeks later.


  • Understand the utility of model organisms in research on developmental mechanisms
  • Understand the conceptual importance of somatic cell nuclear transfer (cloning) experiments
  • Understand the concept of how lineage restriction is controlled by the expression of DNA-binding transcription factors
  • Brief understanding of how transcription factor expression can be controlled by signaling pathways
  • Brief understanding of cell movements in development
  • Brief understanding of how apoptosis can create shape
  • Brief understanding of extracellular matrices in development

Lecture notes

Development Lecture 2010 6images/page for printing (PDF)

Development Lecture 2010 1image/page(PDF)

Lecture Summary

  • Messengers and Receptors
  • chemical signals
  • cellular receptors
  • signal transduction
  • intracellular pathways
  • second messengers
  • Examples of signaling

Honours Projects - Dr Steve Palmer

Project 1: Characterising genes involved in the neurocognitive/behavioural disorder Williams syndrome. Two genes that are disrupted in the human condition Williams syndrome, Gtf2ird1 and Gtf2i, are implicated in aspects of human cognition and behaviour. Molecular genetic techniques and knockout mouse models will be used in this study to investigate the function of these genes. These proteins localize to the nucleus and although their role is not yet defined, the cell biology of this project relates to DNA binding proteins, gene regulation, nuclear function and chromatin organization e.g. histone modification by methylation. In addition, we are doing some neurobiology on the knockouts including starting up a collaboration with Gary Housely using 2-photon imaging to look at neuroanatomy of the cerebellum, hearing tests, electrophysiology and behaviour.

Project 2: Identification of genes involved in the specification of muscle fibre types. We have genetically altered the ratio of fast and slow twitch fibres in transgenic mice. This study will use molecular genetics and proteomics to identify the pathways that decide muscle fibre type. The cell biology aspect of this relates to the determination, development and differentiation of muscle fibres and their sub-specialization into specific twitch types. Also, the adult plasticity of skeletal muscle – ability to atrophy/hypertrophy and change fibre type in response to work demands.


2010 Course Content

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cell Mitochondria | Cell Junctions | Cytoskeleton Introduction | Cytoskeleton 1 Intermediate Filaments | Cytoskeleton 2 Microtubules | Cytoskeleton 3 Microfilaments | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | Revision

Laboratories: Introduction to Lab | Microscopy Methods | Preparation/Fixation | Immunochemistry | Cell Knockout Methods | Cytoskeleton Exercise | Confocal Microscopy | Microarray Visit | Tissue Culture 1 | Tissue Culture 2 | Stem Cells Lab | Stem Cells Analysis

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G