User:Z5158862

From CellBiology

Welcome to Cell Biology 2017!

Lab 1 Assessment

Lab 2 Assessment

  1. Identify a chemical SDS and the risks and hazards of that chemical in text. Add a link to the original SDS
  2. Select 4 reference papers papers related to your selected group project topic sub-section. Read the research papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page.

Lab 3 Assessment - Endo/Exo worksheet questions.

Lab 4 Assessment

  1. Identify a cytoskeletal antibody.
  2. Identify the species deriving the antibody.
  3. Identify the working concentration for the antibody.
  4. Identify a secondary antibody that could be used with this antibody.
  5. Identify a paper that has used this antibody.

This assessment will be due by the next lab (Lab 5).

Lab 7 Assessment

The following peer assessment exercise should be completed before next lab (Lab 8 - 2 May) as your individual assessment for this week (lab missed due to public holiday).

Your answer should be pasted in 2 places

  1. onto each project discussion page (Note you should add anonymously to the discussion page)
  2. your own individual student page for my assessment.

Each individual will provide a brief assessment of the other groups projects. This should take the form of a brief critical (balanced) assessment identifying both the positive (good) and negative (bad) aspects of the project page as it currently exists online.

You may if you choose, use the final project assessment criteria as a guide. Though you are also welcome to use your own criteria.

Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.


Attendance

Z5158862 (talk) 16:07, 7 March 2017 (AEDT)

Z5158862 (talk) 15:35, 14 March 2017 (AEDT)

Z5158862 (talk) 16:38, 21 March 2017 (AEDT)

Z5158862 (talk) 16:07, 28 March 2017 (AEDT)

Z5158862 (talk) 16:49, 4 April 2017 (AEST)

Z5158862 (talk) 15:08, 11 April 2017 (AEST)

Z5158862 (talk) 14:59, 2 May 2017 (AEST)

Z5158862 (talk) 16:33, 9 May 2017 (AEST)

Z5158862 (talk) 16:20, 16 May 2017 (AEST)

Z5158862 (talk) 15:07, 23 May 2017 (AEST)


Tutorial

Student Image Template

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.


Referencing

https://www.ncbi.nlm.nih.gov/pubmed

PubMed

Search Plasma Membrane

  • No Link - PMID: 28257417
  • Link - PMID 28257417

Javier U Chicote, Rob DeSalle, Antonio García-España Phosphotyrosine phosphatase R3 receptors: Origin, evolution and structural diversification. PLoS ONE: 2017, 12(3);e0172887 PubMed 28257417


Image

Human R3 RPTP members.png

Individual Assessment

Lab 1

Histopathologic alteration of peritoneal membranes associated with tumor invasion (H&E and VVG).png

Histopathologic alteration of peritoneal membranes associated with tumor invasion (H&E and VVG)

Reference: Chen J-H, Borges M (2017) Histopathology and enhanced detection of tumor invasion of peritoneal membranes. PLoS ONE 12(3): e0173833. doi:10.1371/journal.pone.0173833

Copyright: © 2017 Chen, Borges. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

(5/5)

Lab 2

Oxalis Chloride

Pictogram:

  • Skull with crossbones- indicating exposure to this chemical can cause death or toxicity.
  • Corrosion- indicating corrosive damage to metals, as well as skin

Hazards:

  • Causes severe skin burns and eye damage
  • Toxic if inhaled
  • May cause respiratory irritation

http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=AU&language=en&productNumber=O8801&brand=ALDRICH&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Faldrich%2Fo8801%3Flang%3Den


Articles on Beta Cells:

Article 1:

Link: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0040049

Citation: MacDonald PE, Rorsman P (2006) Oscillations, Intercellular Coupling, and Insulin Secretion in Pancreatic β Cells. PLoS Biol 4(2): e49. doi:10.1371/journal.pbio.0040049

Title: “Oscillations, Intercellular Coupling, and Insulin Secretion in Pancreatic β Cells”

Summary:

This article focuses on the function of Pancreatic B cells, how insulin is secreted into the circulatory system, and the effects of insulin on our body. Pancreatic B cells are the most abundant cells within the islets of Langerhans of the Pancreas. Its function is to release insulin into the bloodstream in order to regulate blood glucose level. Insulin is secreted from B cells by stimulus-secretion coupling. This process is dependent on electrical activity and Calcium (Ca2+) entry into B cells, via channels in the membranes. This article also covers, in detail, information on uncoupling protein (UCP2), ATP production in B cells, oscillations, how they affect the secretion of insulin by B cells, and how some of these processes can lead to diabetes.


Article 2:

Link: http://journals.plos.org/plosmedicine/article?id=10.1371/journal.pmed.0010058

Citation: Naftanel MA, Harlan DM (2004) Pancreatic Islet Transplantation. PLoS Med 1(3): e58. doi:10.1371/journal.pmed.0010058

Title: “Pancreatic Islet Transplantation”

Summary:

The focus of this article is on discovering new and better treatments for patients with diabetes, specifically type-1 diabetes mellitus (T1DM). Islet transplantation is one of the many treatments currently under experiments and seems to be the most promising. The organ donor’s pancreas undergoes a process where the exocrine functioning part of the organ is removed and the pancreatic islets (responsible for the endocrine function) is isolated. The isolated pancreatic islets are then placed in a vein and transported by blood to the liver. The islets will then be able to produce and secrete insulin. This treatment also has limitations and dangers. And for this reason it is still under experiment and not entirely suggested for diabetic patients everywhere.


Article 3:

Link: http://journals.plos.org/plosmedicine/article?id=10.1371/journal.pmed.0030215

Citation: Eventov-Friedman S, Tchorsh D, Katchman H, Shezen E, Aronovich A, Hecht G, et al. (2006) Embryonic Pig Pancreatic Tissue Transplantation for the Treatment of Diabetes. PLoS Med 3(7): e215. doi:10.1371/journal.pmed.0030215

Title: “Embryonic Pig Pancreatic Tissue Transplantation for the Treatment of Diabetes”

Summary:

This article also focuses on the idea of transplant for the treatment of diabetes, specifically using embryonic pig pancreatic tissue. The study in this article focuses on the growth potential, functionality, and immunogenicity of the embryonic pig pancreatic tissue harvested at different stages of development. After many trials, E42 (embryonic day 42) pig pancreas seems to be the most promising pig tissue for transplantation. It can enable massive growth of pic islets for a long period of time and shows reduced immunogenicity.


Article 4:

Link: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0050163

Citation: Brennand K, Huangfu D, Melton D (2007) All β Cells Contribute Equally to Islet Growth and Maintenance. PLoS Biol 5(7): e163. doi:10.1371/journal.pbio.0050163

Title: All β Cells Contribute Equally to Islet Growth and Maintenance

Summary:

This article focuses on the contribution of B cells to islet growth and maintenance. Whether or not all B cells contribute equally to cell growth, regeneration, and maintenance was tested in this article. Based on the two experiments performed (label-retaining analysis and clonal analysis) the results show that all B cells contribute equally to islet growth and maintenance. B cells are the major source of new B cells (by replication), and not stem cells, which was thought to be the major source of B cells growth and regeneration.

(10/10)

Lab 3

Beta Catenin Polyclonal Antibody

  • Tested species reactivity: Chicken, Human, Mouse, Rat, Xenopus
  • Host/ Isotope: Rabbit/ IgG
  • Polyclonal
  • Unconjugated
  • Form: Liquid
  • Concentration: 0.25 mg/ml
  • Secondary Antibody: Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
  • Beta Catenin Polyclonal Antibody was used to study the role of Plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in rat testis.

Link to article: https://www.ncbi.nlm.nih.gov/pubmed/26048141

Link to Beta Catenin Polyclonal Antibody: http://www.thermofisher.com/antibody/product/beta-Catenin-Antibody-Polyclonal/71-2700

Link to Secondary Antibody: http://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11034

(4.5/5)

Lab 5

Cytoskeleton Laboratory Exercise

Lab 5 Table.jpg

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.


Lab 7

Group 1 (Delta):

Positive:

(1) I love the idea of including short, informative videos on the page. Videos are a great way to teach. They’re quick and easy to understand since you are watching and listening to the information at the same time. One suggestion I have would be to add a brief description above or below each of the videos to let the viewers know what each video is about before watching it. Another suggestion would be to perhaps add copyright information below each video (similar to what we do with images)? (2) I also really like that information on signs and symptoms of each of the diseases are placed in a table. It’s an organized way to put information together. It also helps to capture the attention of the viewers and they are more likely to read them.

Negative/ suggestions:

(1) There are too many headings and subheadings. This can be confusing or overwhelming for some viewers. Decreasing the amount of subheadings on the page will make it appear more organized and easier for viewers to follow through. (2) Some of the sections are written/ typed in different styles. For instance, information under Cell Morphology are in bullets while others aren’t. Keeping the overall text in the same style will help improve the overall look of the page. It will look more organized and the information will flow better. Overall: Compared to the other groups, your project is the most progressed. You have a lot of good information on cell matrix interactions and pathology. You’ve also incorporated, tables, images, and videos nicely into the text. Continue to add more information to the Cell Structure and Development, as well as to the Glossary. Also, work on organizing your contents. Great job so far!

Group 2 (Duct):

Positive:

(1) The introduction is a little shorter compared to those of Group 1 and Group 3, however, it is still good one. It gets straight to the point. It gives a brief, clear explanation of what the pancreas is, what ductal cells are, and the main focus of the project. (2) The images used are very helpful and go well with the information you have for Structure and Signaling. A suggestion I have would be to add a caption for each image and perhaps copyright information (how we did for the first assessment).

Negative/ suggestions:

(1) I’m a little confused why the “Research” heading is before “Introduction” and the other headings, when there is also a “Current Research” subheading. Are they the same thing? If not, then perhaps rename the “Research” heading and move it somewhere after “Introduction”. (2) I suggest placing your “History” information in a table or a time line format, rather than dashes or bullets. This way the section will be more organized and easier for the viewers to read. Overall: Compared to some of the other groups, there are still a lot information that need to be added to your project. The project is well organized, with some small details to fix or reconsider. The information you have right now, especially under “Function”, is very well written. You guys are off to a great start so far!

Group 4 (Alpha):

Positive:

(1) Good start to the “History” section. I like the use of the table and organizing the information by year. (2) I like that you also have a lot of images up compared to the other groups. You just need to elaborate on them more. For instance, add a caption to each of the images.

Negative/ Suggestions:

(1) The videos are great and easy to understand. In my opinion, I think they would look better on the page if they are expanded and are visibly shown on the page (like what Group 1 did on their project). When I first read through your project I missed the videos completely. Viewers will be able to notice the videos and watch them if they are right there on the page. I also suggest adding a brief description of each video on what it is about. Overall: Your group project is the least progressed compared to the other groups right now. I can see that you did do a lot of research on the other topics/ headings (“Pancreas Development, “Signalling”, etc.) by the bullets and quick notes. Once you organize the information into sentences and paragraphs, I’m sure your project will be really great!


very good feedback to the groups. (8/10)



Mark Hill (talk) 20:51, 24 April 2017 (AEST) This lab assessment will be marked by the guest presenter and the mark added here when I have received.



2017 Course Content

Moodle

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2017 Revision

2017 Laboratories: Introduction to Lab | Fixation and Staining |


2017 Projects: Group 1 - Delta | Group 2 - Duct | Group 3 - Beta | Group 4 - Alpha

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G