User:Z5075505

From CellBiology

Welcome to Cell Biology 2017!

Lab 1 Assessment

Lab 2 Assessment

  1. Identify a chemical SDS and the risks and hazards of that chemical in text. Add a link to the original SDS
  2. Select 4 reference papers papers related to your selected group project topic sub-section. Read the research papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page.

Lab 3 Assessment - Endo/Exo worksheet questions.

Lab 4 Assessment

  1. Identify a cytoskeletal antibody.
  2. Identify the species deriving the antibody.
  3. Identify the working concentration for the antibody.
  4. Identify a secondary antibody that could be used with this antibody.
  5. Identify a paper that has used this antibody.

This assessment will be due by the next lab (Lab 5).

Lab 7 Assessment

The following peer assessment exercise should be completed before next lab (Lab 8 - 2 May) as your individual assessment for this week (lab missed due to public holiday).

Your answer should be pasted in 2 places

  1. onto each project discussion page (Note you should add anonymously to the discussion page)
  2. your own individual student page for my assessment.

Each individual will provide a brief assessment of the other groups projects. This should take the form of a brief critical (balanced) assessment identifying both the positive (good) and negative (bad) aspects of the project page as it currently exists online.

You may if you choose, use the final project assessment criteria as a guide. Though you are also welcome to use your own criteria.

Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.

Individual Assessments

Lab 7

Group 2 (Ductal Cells)

This is really easy to understand and read through so far. For the structure sub-heading, the background information on the pancreas’ functions could perhaps go under “Introduction”. An explanation would be very helpful for the diagram. The explanations under your "functions" sub-heading are great. They flow very well and it’s easy to follow. For the history sub-heading, as mentioned above, a table format for years/major events would be useful, or even a timeline image. For the development sub-heading, the brief description of pancreatic development as background was an excellent addition. Overall, the content is clear and concise. Of course, referencing, spell-check, adding images etc. will be done at the end. There are a lot of interesting facts that make this wiki engaging.

Group 3 (Beta Cells)

The introduction is excellent – it gives an overview of the entire pancreas and relevance of the organ. The history section is organised really nicely. The structure section is also very well done. Comparison of mouse and human pancreatic structure is a great addition. The notes under your function, development and signalling sub-headings seem like great points to discuss. This may be changed towards the end but “what would happen without beta cell function” could be included under the “Role in Disease” section (which it already is). The disease section is excellent. There is adequate coverage of the processes that contribute to both types of diabetes. The video and image are relevant and link back to your content directly. Including treatments, especially the stem cell information, complements the disease section well. Another sub-heading for the future of beta-cell research or unanswered questions could be a good addition. Overall, the content on this page isn’t too complicated and easy to understand.

Group 4 (Alpha Cells)

The main headings are suitable and, when content is added, will make the wiki easier to navigate. Development is very well set out. It seems a little dense in terms of sub-headings but once the information is written out I’m sure it will make reading easier. The videos under “function and role” are excellent additions. A brief summary of each of them or referring to them in the text will make it easier to link information together. *The diseases and abnormalities section is also well done so far. Although it hasn’t been put together yet the notes and dot points under each section show that there is good research and presentation of the different aspects of Alpha-cells.


Mark Hill (talk) You have provided some useful feedback, though you seem to say similar things for each project page. You might consider some more critical assessment, as well as identifying the good parts of each project page. (7/10)

Lab 5

Lab 4.JPG

Mark Hill (talk) 20:51, 24 April 2017 (AEST) This lab assessment will be marked by the guest presenter and the mark added here when I have received.

Lab 4

1. Identify a cytoskeletal antibody.

gamma Actin Antibody


2. Identify the species deriving the antibody.

The antibody is derived from a rabbit host.


3. Identify the working concentration for the antibody.

0.2 mg/ml


4. Identify a secondary antibody that could be used with this antibody.

Goat anti-Rabbit IgG secondary antibody


5. Identify a paper that has used this antibody.

Nathan H Blewett, James R Iben, Sergei Gaidamakov, Richard J Maraia La deletion from mouse brain alters pre-tRNA metabolism and accumulation of pre-5.8S rRNA, with neuron death and reactive astrocytosis. Mol. Cell. Biol.: 2017; PubMed 28223366


Mark Hill (talk) 20:43, 24 April 2017 (AEST) Very good (5/5) I hope you recognise gamma actin as one of the 5 mammalian actin isoforms.

Lab 2

1. Identify a chemical SDS and the risks and hazards of that chemical in text. Add a link to the original SDS.

Sodium Cyanide

Hazards:

  • H300 Fatal if swallowed.
  • H310 Fatal in contact with skin.
  • H315 Causes skin irritation.
  • H318 Causes serious eye damage.
  • H330 Fatal if inhaled.
  • H372 Causes damage through organs through prolonged or repeated exposure.
  • H400 Very toxic to aquatic life.
  • H410 Very toxic to aquatic life with long lasting effects.
  • AUH032 Contact with acids liberates very toxic gas.


2. Select 4 reference papers papers related to your selected group project topic sub-section. Read the research papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page.

  • Paper 1

Astrid C Hauge-Evans, Aileen J King, Danielle Carmignac, Carolyn C Richardson, Iain C A F Robinson, Malcolm J Low, Michael R Christie, Shanta J Persaud, Peter M Jones Somatostatin secreted by islet delta-cells fulfills multiple roles as a paracrine regulator of islet function. Diabetes: 2009, 58(2);403-11 PubMed 18984743

Findings: This study explores the role of somatostatin (SST) secreted by pancreatic δ-cells on the function of α- and β-cells. It stems from the knowledge that exogenous SST has an inhibitory effect on insulin and glucagon secretion by these cells. Using a radioimmunoassay on SST knockout mouse models, it was found that absence of δ-cell-derived SST resulted in increased levels of insulin and glucagon in islets in vitro. The influence of exogenous SST in vivo proved difficult to completely remove but the study states both in vivo and in vitro measurements of hormone secretion indicated an inhibitory effect associated with SST secreted by δ-cells.

Relevance: Provides information on the paracrine role of δ-cells.


  • Paper 2

J Morisset, H Wong, J H Walsh, J Lainé, J Bourassa Pancreatic CCK(B) receptors: their potential roles in somatostatin release and delta-cell proliferation. Am. J. Physiol. Gastrointest. Liver Physiol.: 2000, 279(1);G148-56 PubMed 10898757

Findings: This study highlights the effect of pancreatic cholecystokinin (CCK) interaction with CCK(A) receptors on enzyme secretion and cell proliferation within the organ. It used various techniques to assess whether CCK binding to the second receptor, CCK(B), produced similar results. Through the analysis of rat, mouse, pig and human pancreatic islets it was determined that CCK(B) receptors are found specifically, and in high numbers, on foetal human δ-cells.

Relevance: Provides information on the structure of δ-cells.


  • Paper 3

S O Göpel, T Kanno, S Barg, P Rorsman Patch-clamp characterisation of somatostatin-secreting -cells in intact mouse pancreatic islets. J. Physiol. (Lond.): 2000, 528(Pt 3);497-507 PubMed 11060127

Findings: Paper 3 describes the electrophysical activities of pancreatic δ-cells in situ. Of relevance to our sub-topic; the experimental findings indicate that somatostatin release by these cells occurs through Ca2+-dependent exocytosis. This conclusion was based on the increased electrical activity of δ-cells observed when they were exposed to hyperglycaemic conditions.

Relevance: Provides information on factors that regulate δ-cell activity.


  • Paper 4

S Komatsu, M Yamamoto, K Arishima, Y Eguchi Ontogeny of somatostatin cells in the rat fetal pancreas. J. Vet. Med. Sci.: 1997, 59(12);1165-6 PubMed 9450250

Findings: This is a very small study on the development of pancreatic δ-cells in rats. An analysis of foetal rat pancreases at various ages showed the movement of δ-cells from an even distribution across islets at 12-13 days, to a peripheral organisation at day 17. They then migrated further, with some cells found in the pancreatic duct system. δ-cells appeared later than α-cells and around the same time as β-cells.

Relevance: Provides basic information on the changing organisation of δ-cells during development of the pancreas.


Mark Hill (talk) 19:20, 24 April 2017 (AEST) Very good (10/10) For the SDS you could have also identified the Globally Harmonized System. The 4 papers are relevant, hopefully they can be used in the final group project.

Lab 1

TonB and FepA in E coli membranes.png

Distribution of TonB and FepA in membranes of E. coli at high and low concentrations of active fluorescent markers


Reference: Yoriko Lill, Lorne D Jordan, Chuck R Smallwood, Salete M Newton, Markus A Lill, Phillip E Klebba, Ken Ritchie Confined Mobility of TonB and FepA in Escherichia coli Membranes. PLoS ONE: 2016, 11(12);e0160862 PubMed 27935943


Copyright: © 2016 Lill et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.Mark Hill (talk) 12:09, 28 March 2017 (AEDT) All good with the image upload. You only needed to add the image a title and the reference to your page (as below) the additional information goes with the image file. (5/5)



Distribution of TonB and FepA in membranes of E. coli at high and low concentrations of active fluorescent markers[1]

Tutorial

Student Image Template

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

Referencing

PubMed

  • PMID 28253370

Michael P Rout, Samson O Obado, Sergio Schenkman, Mark C Field Specialising the parasite nucleus: Pores, lamins, chromatin, and diversity. PLoS Pathog.: 2017, 13(3);e1006170 PubMed 28253370


JCB Copyright:

Rockefeller University Press Copyright Policy This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

Image

Human R3 RPTP members.png

Name of Image

Reference: Michael P Rout, Samson O Obado, Sergio Schenkman, Mark C Field Specialising the parasite nucleus: Pores, lamins, chromatin, and diversity. PLoS Pathog.: 2017, 13(3);e1006170 PubMed 28253370


Attendance

Lab 1: Z5075505 (talk) 16:19, 7 March 2017 (AEDT)

Lab 2: Z5075505 (talk) 15:35, 14 March 2017 (AEDT)

Lab 3: attended

Lab 4: Z5075505 (talk) 15:37, 28 March 2017 (AEDT)

Lab 5: Z5075505 (talk) 16:09, 4 April 2017 (AEST)

Lab 6: Z5075505 (talk) 15:08, 11 April 2017 (AEST)

Lab 7: Anzac Day

Lab 8: Confocal Lab

Lab 9: Z5075505 (talk) 15:24, 9 May 2017 (AEST)

Lab 10: Z5075505 (talk) 15:38, 16 May 2017 (AEST)

2017 Course Content

Moodle

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2017 Revision

2017 Laboratories: Introduction to Lab | Fixation and Staining |


2017 Projects: Group 1 - Delta | Group 2 - Duct | Group 3 - Beta | Group 4 - Alpha

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G
  1. Yoriko Lill, Lorne D Jordan, Chuck R Smallwood, Salete M Newton, Markus A Lill, Phillip E Klebba, Ken Ritchie Confined Mobility of TonB and FepA in Escherichia coli Membranes. PLoS ONE: 2016, 11(12);e0160862 PubMed 27935943