- 1 Mark's Next Big Thing
- 2 Lab One Individual Assessment
- 3 Lab Two Individual Assessment
- 4 Lab Three Individual Assessment
- 5 Summary of Super Resolution Microscopy
- 6 Paraformaldehyde MSDS
- 7 Lab 5 Individual Assessment
- 8 Lab 6 Individual Assessment
Mark's Next Big Thing
Not So Big Thing
Cells_Eukaryotes_and_Prokaryotes PMID 25513760
Lab One Individual Assessment
Lab Two Individual Assessment
Relative Lineage of Bifidobacterium Species From a Range of Organisms.
Lab Three Individual Assessment
Integrin traffic – the Update
This article discusses the complex topic of integrin trafficking and the important role it plays in the endocytic and exocytic maintenance and recycling of integrin-ECM adhesions that anchor the ECM to the plasma membrane. It also identifies the role that focal adhesions play in maintaining connections to the ECM. The role that cholesterol plays in regulating these focal adhesions is also discussed.
Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner
This article tackles the role that integrin-laminin interactions play in the ECM and how external mechanotransduction influences the fate of stem cell differentiation. It also discusses the role that integrin play as mechanosensors for the cell, and how static stretch plays a influential role in the differentiation of stem cells. The paper concludes with stating that further research into the role that laminin integrin and laminin serve as a mechanosensor.
Vascular Smooth Muscle Cell Stiffness and Adhesion to Collagen I Modified by Vasoactive Agonists
This article discusses how integrins, specifically collagen binding integrins, play a key role in the contraction of vascular smooth muscle cells. Furthermore, the researchers hypothesize that integrin-collagen adhesion is regulated by the initiation of contraction within vascular smooth muscle cells. The paper’s findings suggest that it is the integrin adhesion sites that provide sound stability for the contraction of vascular cells.
Tumor Angiogenesis in the Absence of Fibronectin or Its Cognate Integrin Receptors
This articlediscusses the possible role that integrin and fibroconectin binding to the ECM could contribute to cell growth, and cancer growth specifically. The researchers studied the critical roles that integrin and their association with ECM fibronectin proteins play in mouse embryo development. The researchers concluded that integrin interactions are completely necessary for tumor angiogenesis in mice.
Summary of Super Resolution Microscopy
In the research article Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer , the investigators discuss how the recent advancement of super resolution microscopy has allowed them to more closely analyse the structure of the immunological synapse. To do this, investigators implemented the use of the glass-supported lipid bilayer technique to act in place of the natural killer cells on which the immunological synapse naturally resides. This alternative protocol was implemented in order avoid issues seen in similar investigations that received only images of the complex "in profile". By utilizing both the glass-supported planar lipid bilayer and the more detailed super resolution microscopy, the investigators devised a protocol that aimed to a achieve a more useful representation of the immunological synapse, than had been achieved in previous investigations.
The investigation was carried out after completing the abridged protocols, and the resulting product was then imaged using STED microscopy, using florescent markers as guides for targeted complexes. While this set up was compliant with the expected results of the investigators they then acknowledged several faults within their experimental design. While the use of glass-supposrted planar lipid bilayer surface has its benefits, the researches admit that because is its a synthetic medium it does not account for naturally occurring protein structures, i.e integral proteins, cytoskeleton etc., there may be some variation between their achieved representation and the natural structure of a immunological synapse on a natural killer cell.
Paraformaldehyde is a chemical compound that is flammable, toxic, carcinogenic, irritating, and causes burns in high concentrations. [Paraformaldehyde MSDS]
Lab 5 Individual Assessment
Lab 6 Individual Assessment
Identify an antibody that can been used in your group's extracellular matrix project.
Integrin Alpha 5 Anti-body 17E6
Identify the species deriving the antibody.
Identify the working concentration for the antibody.
Identify a secondary antibody that could be used with this antibody.
Rabbit anti-Mouse IgG (H+L) SuperClonal™ Secondary Antibody
Identify a paper that has used this antibody.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Growth Media: A 1:1 mixture of Dulbecco's Modified Eagle's Medium (With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture:http://www.sigmaaldrich.com/catalog/product/sigma/d6046?lang=en®ion=AU) and Ham's F12 medium ( with 2.5 mM L-glutamine, 1.2 g/L sodium bicarbonate, 15mM HEPES and 0.5mM sodium puruvate supplemented with: 0.010 mg/ml bovine insulin 0.010 mg/ml human transferrin 1% chemically defined lipids (LifeTechnologies cat.# 11905-031) 50 ng/ml mouse epidermal growth factor 10 nM sodium selenite
Growth Media: ATCC iPSCs have been adapted to feeder- and serum-free culture conditions.
Peer Critiques of Group Projects: Project 1 Critique: I really enjoy the image you have at the top of your page, I think it’s really eye catching and it really helps engage in the beginning of your project. I think the first section of your project really hits the ground running; maybe a more generalized overview of proteoglycans, highlighting the aspects you will be discussing in your project would make for a better introduction to your project. I really like the organization of your history section I think it works really well. The information in your structure section is good, and the diagrams are great, but to be nit-picky with so many diagrams having a variance in color would make the diagrams much easier to focus on. I thought your section on roles in disease was really great, the diversity of the types of research studies that you included made the applications of proteoglycans very interesting. It seemed like you are still missing some information in a couple of places, and a few images to break up the bulky text throughout the project, but overall I think your project is in a really good place. Good job!
Project 3 Critique: I really liked that you began your project with a general overview of your topic that was not too complicated or too detailed, it allows the reader to understand what your topic is. However, you may want to also include what aspects of elastic fibres you will be discussing and what you won’t. The way you organized your structure section was really great, but I think an image of a general elastic fibres with the labeled components would be really helpful in understanding how the components fit together. As far as your assembly section goes, the diagrams that you have a re really great, especially the hand drawn diagram, it is eye catching and engaging, well done. I also enjoyed the organization of your function section, how you discussed function in the scope of specific tissues, but I think the images in this section may be just a bit too large, as they result in a very large white space to the right of the images, perhaps just something to consider. I also found your clinical examples and organization of that section to be very good, I enjoyed that you have very distinct examples of clinical implications.
Project 4 Critique: I personally found your introduction to be a bit too lengthy and too detailed for an introduction, I would have enjoyed a brief overview of the molecule, its structure and function, and then discuss how the remainder of your project page was going to elaborate upon the subject or outline the remainder of the topic. Your timeline is a nice component to your project, and if there is a way to possibly change the format to stand out a bit more I think it would really contribute to the look of your project. Also I think you may want to consider an image or diagram for your structure section, it would really help to organize the different components in a labeled fashion. The image that you do have in your structure section I think needs some sort of explanation because I couldn’t understand how it related to this section of your project. I also thought that your function section was a bit broad, perhaps you could refocus this section and outline some of the major functions, because it seems to be a bit lengthy. I liked the information you have in your abnormalities section, but I think the formatting of this section and current research section could be improved to add to the over all appearance of your project.
Project 5 Critique: I thought your introduction was really good, a very nice overview of your topic, but you may want to consider outlining the rest of your project page within your introduction stating what you will and will not be talking about in regards to your topic. I’m also not sure if your bulleted list works in your introduction or not, to me it just seemed a bit out of place. You may also want to reconsider your referencing format I found it to be disruptive to the flow of your project. I liked the information you have in your history section as well, very thorough, but you may want to try to organize it in some fashion, maybe with two sub headings. Similarly, your structure section has wonderful information , and the image really makes the section complete, but in order to get through all of the text a couple of sections within structure would be helpful organization wise. The section “image” doesn’t quite make sense, but the images within that section are very helpful for understanding structure. As far as the specific lamini functions, you may want to keep the sections on major laminins that have a considerable amount of information, and then the laminins that have one line descriptions could be formatted into a table. Your abnormalities section seems to be a bit inconsistent with images, and with the formatting there seems to be quite a bit of white space. I found your current research section to be very thorough, I personally wouldn’t have read through all of it if I didn’t have to, you may want to consider compacting that section a little. But overall the project seems to be coming along pretty well.
Project 6 Critique:
An introduction that gives a fairly general overview regarding your topic would be nice, and perhaps you could also use the introduction section to outline what you intend to discuss in your project. Your history section seems to be a bit too incomplete to use a paragraph format, perhaps there is someway you can make a timeline type format, just something to consider. Your structure section is still lacking in a couple of places, but your image really contributes to the sections that you have. I really enjoyed your function section, it was very concise and thorough.
For your abnormalities section you may want to frame the beginning of the section with a heading, when I started reading the fist body of text in that section I wasn’t exactly sure what I was reading about. If the beginning of this section is meant to be an introduction to the section, perhaps frame it in that manner. Your research section was nice, highlighting a couple of research studies, perhaps could do with another image or two to keep the reader interested but over all a nice job thus far. ‘
Project 7 Critique: You have a great introduction, it has everything I wanted to know regarding what was going to be discussed in your project and included a brief overview of your topic. Your timeline could use a few more entries, but it is a good idea, you may also want to include the interest in basement membrane in research from its discover to today, just a thought. Your section on Formation, plasticity, and regeneration may need a brief introduction as to what the section is going to discuss, it would help frame the bullet points. I really enjoyed your functional layers section, works really well with the image and the bullet points. I diagram may be helpful in explaining your structural components section how this parts interact with the basement membrane, perhaps something to consider. Under functions you seem to have a great deal of text, a couple of sub headings may help to visually break up the information (skin, kidneys etc.). Your abnormalities section is very well balanced between information and images, over all your project is in a pretty good place.
"Lab 12 Assessment"