- 1 Lab attendance
- 2 Week 2: Lab one Individual Assessment
- 3 Reference
- 4 Lab two
- 5 Week 3: Lab Two Individual Assessment
- 6 Lab Three
- 7 Week 4: Lab Three Individual Assessment
- 8 Week 6: Lab Five individual assessment
- 9 Week 7: Lab Six Individual Assessment
- 10 Week 11: Peer Review Assessment
- 11 Week 13: Individual Assessment
Week 2: Lab one Individual Assessment
PepQ forms a canonical pita-bread fold with binuclear active site.
PepQ forms a canonical pita-bread fold with binuclear active site.
Week 3: Lab Two Individual Assessment
The article by Zhong et al. (2014), acknowledges the recent discovery of a new layer underneath the outer membrane of axons, which is composed of actin filaments and is separated by proteins called spectrin. The authors utilised super-resolution microscopy to uncover why and how this lattice-like skeleton membrane is formed in the axons of neurons. . It was found that this skeleton developed early and continued to mature in the life of a neuron. However, it only formed in regions with sufficient amounts of BII spectrin. Axons and dendrites in neurons have a vital role in signalling. Hence, with the new advancement in technology, the authors were able to propose that the distribution of spectrin in only certain regions of the neuron is one way of maintaining specific structures with unique functions in those regions.
- A flammable solid
- Harmful if swallowed
- Causes skin irritation so it can cause an allergic skin reaction
- Can cause serious eye damage
- Harmful if inhaled - can cause respiratory irritation
- Suspected of causing cancer
Week 4: Lab Three Individual Assessment
Collagen Type II: Function
This article suggests that the extracellular matrix of the cartilage is mainly composed of type II collagen. In healthy cartilage, type II collagen is not degraded. However, when it is degraded by enzymes this causes joint damage, this is what occurs in osteoarthritis.
In this article, it says collagen type II along with other proteins, has a role in skeletal development. Collagen II also interacts with minor collagens IX and XI to form heterotypic fibrils. Mutations in type II collagen in the extracellular matrix of the vertebrae show the important structural and developmental role of the fibrillar network.
In this article, it mentions that type II collagen is found in articular cartilage, which acts as a load bearing, low-friction, wear-resistant cushion. It is located at the ends of long bones to allow skeletal movement that is painless.
This article says that type II collagen plays an essential role in both fracture healing and long bone development. It is also mentioned that an increased production of type II collagen could enhance full bone formation. Type II collagen promotes bone marrow derived mesenchymal stem cell (BMSC) osteogenesis and inhibits adipogenesis. This means that collagen II may have a function in the early stage of BMSC differentiation.
Bone and cartilage both contain COL2A1 gene which has a role in the production of type II collagen. Therefore, mutations in such a gene can show many abnormalities.
Radiograph of the Family Members.
Week 6: Lab Five individual assessment
Week 7: Lab Six Individual Assessment
Species deriving the antibody: mice, rats, and chickens.
Working concentration is 0.1mg/ml
Secondary antibody: Collagen II Antibody (M2139)
Article that uses this antibody as well as the secondary antibody: <pubmed>15934095</pubmed>
Week 11: Peer Review Assessment
Group 1: Small Leucine-Rich Proteoglycans Pros: The choices of the sub-headings, tables, diagrams and pictures seem to show a good understanding of the topic area thus far. The pictures for the diseases look interesting and they caught my eye. I really like how you guys got creative and included a banner for your project page. The group has utilised their own innovative diagrams. Cons/improvements: The picture for the proteoglycan molecule is too large and at the very centre of the project page. I feel like you guys might be concentrating on too many diseases? Maybe focus on three interesting ones. You guy have a subheading for decorin and biglycan and then further bold subheadings for them. I feel like this could be better presented without repeating the proteins numerous times.
Group 2: Integrins Pros: You guys seem to be on top of things. It is evident that you guys know where you want to go with this topic. This is shown through your choice of content, headings, sub-headings, diagrams and graphs. Good job with finding a video for your topic! There is use of the student’s own diagrams. There is adequate information in all subheadings of the topic – which shows you guys have really researched thoroughly. Cons/improvements: Im not sure as a reader where you are going with the section underneath the glossary? The last section is a bit too lengthy
Group 3: Elastic Fibres Pros: It is evident you guys have done a lot of research for your topic and the pictures helpful in understanding the topic you guys are exploring. You have utilised your own innovative diagrams.There is an adequate amount of information in each section. The content is referenced Cons/improvements: You guys could improve by using more subheadings. There is no history/current research section. The histology pictures could be better positioned to maintain a flow in the page
Group 4: Fibronectin Pros: The project page is well organised and has a sufficient amount of information under each heading. The topic seems well maintained and there is a flow throughout the page in terms of content. There is proper use of referencing Cons/improvements: You guys could definitely make the project page more interactive by including diagrams, videos, and graphs. You would also benefit from the use of a glossary You guys haven’t made your own diagram/picture yet so maybe work on that.
Group 5: Laminin Pros: There is a good understanding of the topic area – this is shown through the amount of information provided on your page. You guys are really researching your topic well – there are lots of subheadings with paragraphs of information. Cons/improvements: The page seems all over the place at the moment – you have references under each section (there should only be one reference located at the bottom of the page). The information needs to be cut down and made more concise so you guys can maintain a good flow to what you want to teach about your topic. You guys should really utilise more pictures and diagrams as well as a hand drawn diagram.
Group 7: Basement Membrane Pros: Your project page has a good amount of information that is balanced under each heading. You guys broken down the topic into subheadings and this really shows that you know where you want to go with your chosen topic. I like how almost every section has a picture accompanied with it – makes it very interesting to read. There is correct use of referencing. Cons/improvements: You guys don't have a glossary or a hand drawn diagram. The function section seems a bit too chunky – maybe include some small subheadings within it.
Week 13: Individual Assessment
Whole-exom sequencing was performed through illumina sequencing. It showed mutations in the collagen type II gene COL1A1.