User:Z3465531

From CellBiology

My Student Page

The following is the UNSW ANAT3231 Cell Biology student page for z3465531:

Attendance

Z3465531 (talk) 11:53, 10 March 2016 (AEDT)

Z3465531 (talk) 11:06, 17 March 2016 (AEDT)

Z3465531 (talk) 14:39, 24 March 2016 (AEDT)

I forgot to submit the above attendance until I returned home from the lab.

Z3465531 (talk) 11:13, 7 April 2016 (AEST)

Z3465531 (talk) 11:12, 14 April 2016 (AEST)

Z3465531 (talk) 11:27, 21 April 2016 (AEST)

Z3465531 (talk) 11:08, 28 April 2016 (AEST)

Z3465531 (talk) 11:06, 5 May 2016 (AEST)

Z3465531 (talk) 11:07, 12 May 2016 (AEST)

Z3465531 (talk) 11:11, 19 May 2016 (AEST)

Z3465531 (talk) 05:48, 28 May 2016 (AEST)

The presentations were very engaging, and I completely forgot to submit the above attendance until the weekend following the lab. I was, however, present for all of the group presentations, as well as participating in my own group's presentation.

Z3465531 (talk) 11:12, 2 June 2016 (AEST)


  • Z8600021 12 practicals listed as attended. You were supposed to enter your attendance at the beginning of every class.

Weekly Assignment

Group Projects
This year's main topic is Blood Cell Biology. Each group should discuss with group members the specific sub-topic that will be covered by their project.

Here is a list of some of the cell types (Structure and Function)

Cell Type (PuMed citations)


Below are the groups to which students have been randomly assigned. You should now on the project discussion page add your own suggestion for a specific topic. Once your group has agreed on the topic, add this as a heading to the project page before Lab 3.


2016 Projects: Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7

Group 1: User:Z5017493 | User:Z3330991 | User:Z5020043 | User:Z5020175 | User:Z3489355

Group 2: User:Z5018320 | User:Z5015980 | User:Z3376375 | User:Z3461106

Group 3: User:Z5019595 | User:Z5019962 | User:Z5018925 | User:Z3461911

Group 4: User:Z5020356 | User:Z3463895 | User:Z3376502 | User:Z3423497 | User:Z5021149

Group 5: User:Z5015719 | User:Z3462124 | User:Z3463953 | User:Z5017292

Group 6: User:Z5018866 | User:Z3329177 | User:Z3465531 | User:Z5105710

Group 7: User:Z5021060 | User:Z5016365 | User:Z5016784 | User:Z3414546 | User:Z3417773

Group Assessment Criteria

Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.
Individual Lab Assessments
Lab 8 Assessment
2016 Lab 8 - Lab 8 Assessment (to be completed before Lab 9)
  1. Add your peer assessment to your own student page to the site.
  2. Add your peer assessment to each project discussion page to the site.
Lab 6 Assessment
2016 Lab 6 -
  1. Identify an antibody against your group blood cell protein that is commercially available.
  2. Add a link to the original data sheet page and identify the type of group blood cell protein.
  3. Include the following information: type of antibody (polyclonal, monoclonal), species raised in, species reacts against, types of application uses, and if available any reference using that antibody.
Lab 2 Assessment
2016 Lab 2 - Super resolution microscopy
  1. Find a recent research article (not review) that uses super resolution microscopy technique.
  2. Write a brief summary of the paper (referenced) and what the super resolution microscopy technique showed.
    1. This should not simply be the abstract of the paper.
    2. This can be 2-3 paragraphs no longer.
  3. Include a super resolution microscopy image from the paper.
    1. Therefore the paper must be from a source that you can reuse.
    2. Image uploaded as in Lab 1 (summary box - description/reference/copyright/student image)
    3. Image should appear as a "thumbnail" (thumb) next to your paper summary (with citation legend) See Test page
Lab 1 Assessment
2016 Lab 1 - Lab 1 Assessment (to be completed before Lab 2) The test page I set up in the Lab
  1. Add your own student page to the site.
  2. Add your signature for Lab attendance.
  3. Add a sub-heading.
  4. Add an external Link.
  5. Add an internal Link.
  6. Add an image from PubMed, PloS or BioMed Central journal related to prokaryote cellular component. Make sure it includes both the reference and copyright information, with the file and where it appears on your page.

To Do: Find a research article and write a brief summary. Upload an image of the super resolution as a thumbnail sitting next to the shortcut. The image has to be taken from an allowable article.

Lab 1 Assessment

Search PubMed

prokaryotic cytoskeleton

http://www.ncbi.nlm.nih.gov/pubmed/?term=eukaryotic+cytoskeleton

PMID 26756351

Katherine Ann Hurley, Thiago M A Santos, Gabriella M Nepomuceno, Valerie Huynh, Jared T Shaw, Douglas B Weibel Targeting the bacterial division protein FtsZ. J. Med. Chem.: 2016; PubMed 26756351


BioMed Central

How to make an in-text citation

Bacterial division protein FtsZ.[1]

Links

Testz8600021

Carnegie stage table

Cell_Biology_Introduction

Lecture 1

SMH Sydney Paper

What I have learned so far

The most useful information I have learned so far is multiple specific computer programming language commands necessary for organizing information on wiki pages. In particular, I have learned the incredibly useful command for instantly creating a citation of a PubMed article. I have also learned how to rename links a wiki page so that full website address itself is not necessarily listed and is instead whichever name I select. The simple coding language use of equals signs will be extremely helpful for organizing my work into simple headings and subheadings. Additionally, I learned that all of the edits to the wiki will be completely preserved so that there is no risk of information and progress being lost. In general, I learned how to execute several wiki commands, and now I have a useful template available for the purposes of creating wiki pages throughout the rest of the course.

Student Image

Here is the uploaded image


  • Z8600021 try and set a smaller size for your image appearance on your page.


Bacterial Phylogenic Tree Example.png

Bacterial phylogenetic tree reconstructed from a concatenated alignment of 50 nearly ubiquitous r-proteins[2]

You can also set it to any size you want, this is 200px wide.

Bacterial Phylogenic Tree Example.png]

Bacterial phylogenetic tree reconstructed from a concatenated alignment of 50 nearly ubiquitous r-proteins[2]

References

  1. Katherine Ann Hurley, Thiago M A Santos, Gabriella M Nepomuceno, Valerie Huynh, Jared T Shaw, Douglas B Weibel Targeting the bacterial division protein FtsZ. J. Med. Chem.: 2016; PubMed 26756351
  2. 2.0 2.1 Natalya Yutin, Pere Puigbò, Eugene V Koonin, Yuri I Wolf Phylogenomics of prokaryotic ribosomal proteins. PLoS ONE: 2012, 7(5);e36972 PubMed 22615861

Lab 2 Assessment

  • Z8600021 Image is suitable and contains all requirements. Your summary describes the paper findings. (5/5)

In their article, Superresolution and Fluorescence Dynamics Evidence Reveal That Intact Liposomes Do Not Cross the Human Skin Barrier, Dreier et al investigate the mechanism by with liposomes act to deliver drugs in human skin. Very few drugs are easily delivered across the skin barrier, but lipid vesicle carriers are one of the methods by which the permeably of human skin is able to be enhanced. Specifically liposomes, also known as large unilamellar vesicles (LUVs), are of particular interest when considering options for drug delivery across the dermis. Despite the widespread use of LUVs commercially, much has historically been unknown about the mechanism of action due to the minute scale at which the liposomes are functioning.

Dreier et al used stimulated emission depletion (STED) microscopy to create superresolution images able to resolve the 100nm liposomes of interest in the skin. STED microscopy allowed for high enough resolution to determine that hardly any liposomes were intact deep to the skin surface. Therefore, Dreier et al were able to conclude that liposomes used for transdermal drug delivery mostly burst fusing to deliver their contents to the outmost layers of the skin rather than directly transporting intact liposomes through the skin barrier. [1]

Thumbnail version of image

You can also set it to a thumbnail image.

Image of the surface of intact skin[1]

References

  1. 1.0 1.1 Jes Dreier, Jens A Sørensen, Jonathan R Brewer Superresolution and Fluorescence Dynamics Evidence Reveal That Intact Liposomes Do Not Cross the Human Skin Barrier. PLoS ONE: 2016, 11(1);e0146514 PubMed 26751684

Lab 3 Assessment

  • Z8600021 Image and summaries are good, I hope you were able to use these in the final project. Late pasting in addition to your lab attendance sign-ins means that you should be thinking about your work flow in class. (5/5)

The following assessment for Lab 3 I completed in the discussion area of our group page before Lab 4 but forgot to copy over to my individual page before Lab 4:

z3465531

1. Summary: Cathepsin B controls the persistence of memory CD8+ T lymphocytes, Byrne, S. M., et al. [1]

This study investigates the mechanisms by which memory T lymphocytes persist, even in the absence of antigen.

The findings indicate that one of the major limiting factors to memory CD8(+) T cell populations is the stability of the cell lysosomes. The genes IL-15 and IL-7 contribute to the production of serine protease inhibitor (Spi) 2A, which inhibits cytosolic cathepsin and thus inhibits apoptosis. In animal models, Spi2A inhibition results in less CD8+ T lymphocyte maturation and less homeostatic proliferation as well. The experimental results indicated that Spi2A is required in order for the memory CD8(+) T lymphocytes to maintain population numbers after viral infection and that one specific mechanism of action is protection from lysosomal breakdown via cathepsin B inhibition. Furthermore, it was found that T cells deficient in Spi2A could have their homeostatis restored by concurrently blocking cathepsin B, which strongly suggested that the physiological target of Spi2A was indeed cathepsin B.

The relevance of this topic to T lymphocytes, specifically memory T lymphocytes, is manifold. The primary function of memory T lymphocytes is to persist within the organism until such time as they are necessary to promote a fast, powerful targeted response to a second infection. If the population of the memory T lymphocytes is allowed to deplete, then immunological memory will not remain. If, on the other hand, memory T lymphocytes proliferated over abundantly, then this would be a likely mechanism for the development of possible leukemia.

2. Summary: The majority of HIV type 1 DNA in circulating CD4+ T lymphocytes is present in non-gut-homing resting memory CD4+ T cells, McBride, K., et al. [2]

This study investigates the locational distributions of integrated HIV-1 DNA copiers within memory CD4+ T lymphocytes for the purposes of better understanding the efficacy of disease treatments.

A proposed site of prominent HIV-1 replication with Memory CD4+ T lymphocytes is the gut-associated lymphoid tissue, where such cells tend to recirculate. The researchers of this study hypothesized that regulatory T cells and activated CD4+ T lymphocytes tend to recirculate and disproportionately make up a source of the HIV-1 reservoir, serving as a difficulty needing to be solved in any possible eradication of the disease.

Real time polymerase chain reaction was used to localise HIV-1 DNA within the human immune cells. More than 80% of total HIV-1 DNA was found in non-gut-homing CD45RO+ memory T lymphocytes, while less than 10% was found in regulatory T cells and CD38+ activated memory cells. The researchers found that most of the HIV-1 DNA was present in non-gut homing resting CD(+) T cells. The findings of the experiment are significant because they work toward better understanding the locational pathogenesis of HIV-1 in memory CD4+ T lymphocytes, which is necessary for developing a cure.

3. Summary: Interleukin-22 is produced by invariant natural killer T lymphocytes during influenza A virus infection: potential role in protection against lung epithelial damages, Paget, C., et al. [3]

During viral infections, invariant natural killer T (iNKT) cells are a type of non-conventional T lymphocyte that plays a key role through quickly producing cytokines. While the mechanism of their action is not entirely well known, it has been proposed that they play a useful role during influenza A virus (IAV) infection.

The findings of the article support that iNKT cells produce IFN-γ and IL-22, cytokines critical to muscosal immunity. The specific mechanisms of the production and mediation of these cytokines are investigated, especially with respect to the morality of those infected by the virus. It was demonstrated in vitro and vivo, at least during early IAV infection, that IL-22 helps to prevent epithelial cell death in a IAV-infection of the airway. In total, iNKT cells were revealed to provide a quick source of IL-22 that possibly is critical to protecting the lung epithelium. These findings are significant to better understanding how T-lymphocytes, and specifically natural killer T lymphocytes, play a role in proactively protecting from early infections in critical exposed areas, such as the lungs.

4. Summary: Microbial exposure during early life has persistent effects on natural killer T cell function, Olszak, T., et al. [4]

This study investigated the importance of microbial exposure at a young age to the function of natural killer T cells, which are supposed to play a critical role in diseases relating to the immune system such inflammatory bowel disease (IBD) and asthma.

The findings of the article are that germ-free (GF) mice tended to accumulate invariant natural killer T (iNKT) cells in the lungs and in the lamina propria of colon, increasing morbidity as compared with specific pathogen-free mice. Also, the GF mice had increased intestinal and pulmonary expression of CXCL16, a chemokine ligand associated with increased iNKT cells of the mucosa.

On the other hand, when neonatal GF mice were colonized with conventional microbiota, the mice tended to be protected from iNKT accumulation within the mucosa and associated pathogenicity. The results of the experiments as a whole suggested that contact with commensal microbes by a critical early age are crucial to developing a healthy levels of mucosal iNKT. These findings are significant to better understanding iNKT cells because they address both the natural function of iNKT cells in providing resistance to environmental exposures, but the experimenters also investigate the double edged nature to this immunity. If a the host fails to interact with any conventional microbiota during the early stages of life, then the natural killer T-cells themselves seem to be a source of pathogenicity. The results of the experiment provide crucial insight for better understanding T lymphocytes as a whole.

Migratory challenges faced by T cells [5]

References

  1. Susan M Byrne, Anne Aucher, Syarifah Alyahya, Matthew Elder, Steven T Olson, Daniel M Davis, Philip G Ashton-Rickardt Cathepsin B controls the persistence of memory CD8+ T lymphocytes. J. Immunol.: 2012, 189(3);1133-43 PubMed 22745374
  2. Kristin McBride, Yin Xu, Michelle Bailey, Nabila Seddiki, Kazuo Suzuki, John M Murray, Yuan Gao, Celine Yan, David A Cooper, Anthony D Kelleher, Kersten K Koelsch, John Zaunders The majority of HIV type 1 DNA in circulating CD4+ T lymphocytes is present in non-gut-homing resting memory CD4+ T cells. AIDS Res. Hum. Retroviruses: 2013, 29(10);1330-9 PubMed 23971972
  3. Christophe Paget, Stoyan Ivanov, Josette Fontaine, Joelle Renneson, Fany Blanc, Muriel Pichavant, Laure Dumoutier, Bernhard Ryffel, Jean Christophe Renauld, Philippe Gosset, Pierre Gosset, Mustapha Si-Tahar, Christelle Faveeuw, François Trottein Interleukin-22 is produced by invariant natural killer T lymphocytes during influenza A virus infection: potential role in protection against lung epithelial damages. J. Biol. Chem.: 2012, 287(12);8816-29 PubMed 22294696
  4. Torsten Olszak, Dingding An, Sebastian Zeissig, Miguel Pinilla Vera, Julia Richter, Andre Franke, Jonathan N Glickman, Reiner Siebert, Rebecca M Baron, Dennis L Kasper, Richard S Blumberg Microbial exposure during early life has persistent effects on natural killer T cell function. Science: 2012, 336(6080);489-93 PubMed 22442383
  5. Loïc Dupré, Raïssa Houmadi, Catherine Tang, Javier Rey-Barroso T Lymphocyte Migration: An Action Movie Starring the Actin and Associated Actors. Front Immunol: 2015, 6;586 PubMed 26635800

Lab 5 Assessment

This chart displays the effect of overexpression of tropomyosin 4 (Tm4) on the percent morphological phenotype. Group A: Overexpression of Tm4, Group B: Control (wildtype; WT).

This chart displays the effect of overexpression of tropomyosin 4 (Tm4) on the percent morphological phenotype. The overexpression of Tm4 appeared to result in increased neurite growth, leading therefore to increased incidence of "pronged" and "stringed" morphology as opposed to the control (wildtype; WT) group. Overexpression of Tm4 led to a significant reduction of all other phenotpyes except "stumped". Cell counts were carried out on "Images for Analysis - Group 1" in 2016 Lab 5 according to the specified guidelines for analysis of phenotype.

References


Lab 6 Assessment

  • Z8600021 All information is here as required, though perhaps you should have selected on e of the many 1000s of the published antibodies as your example. (5/5)


1. Identify an antibody against you blood group cell protein that is commercially viable.

Anti-TCR alpha antibody (ab18861)

The antibody is commercially available from abcam.

2. Link to the original data sheet page:

http://www.abcam.com/tcr-alpha-antibody-ab18861.html

The type of group blood cell protein:

The antibody is rabbit polyclonal to TCR alpha.

3. Identify the type of antibody (polyclonal, monoclonal): polyclonal

Identify the species deriving the antibody: Rabbit

Identify the species the antibody reacts against: No specific species is given, but T cell antigen receptor (TCR) recognizes foreign antigens and then translates any such recognitions into signals within the cell to elicit changes in the cell to transition from dormant to activated.

Identify the working concentration for the antibody.

IHC-Fr: Use at a concentration of 15 - 25 µg/ml. IP: Use at a concentration of 10 - 20 µg/ml. WB: Use at a concentration of 1 - 4 µg/ml.

Identify a secondary antibody that could be used with this antibody.

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique

Performed under reducing conditions.

Identify a paper referencing the use of this antibody.

According to its datasheet, the ab18861 antibody has not yet been referenced specifically in any publications.

References

http://www.abcam.com/tcr-alpha-antibody-ab18861.html

Lab 8 Assessment - Peer Project Reviews

  • Z8600021 These are good peer feedback reviews and you have used some of the set assessment criteria as your guide. (18/20)


Group Assessment Criteria

1. Are the key points of the topic delineated and clearly presented in detail? 2. Are the content, headings and sub-headings, diagrams, tables, graphs appropriate and do they indicate sufficient comprehension of the allocated topic? 3. Are citation and references for the topic appropriate? 4. Is the wiki instructive to peers by making use of insightful diagrams, tables or figures and/or examples or explanations authored by the group members? 5. Is it clear that dedicated research has been conducted to connect basic and applied sciences, and does the effort go beyond the formal class material? 6. Does the group relate the content of the wiki to the primary learning aims of cell biology?

Megakaryocytes Group 1 - Peer review by z3465531

1. Are the key points of the topic delineated and clearly presented in detail?

• The key points of the topic were easily identified as aspects of Megakaryocytes according to the title figure, detailed structured subheadings, and a clear introduction.

• Clear language was often used, but some sections, such as Development and Maturation Process, were more clearly expressed than others.

• As a result, the article was almost always easy to follow and informative.

2. Are the content, headings and sub-headings, diagrams, tables, graphs appropriate and do they indicate sufficient comprehension of the allocated topic?

• The content presented was detailed, appropriate, and was usually likewise presented, but some sections such as Essential Thrombocytosis and Signaling seemed overly technical.

• The figures were appropriate and sufficient, and they helped convey sufficient comprehension of Megakaryocytes.

• The title banner, student authored, makes for a great display of the topic.

• The glossary is a helpful addition to the wiki page.

• Introduction: Just a quick heads up that megakaryocyte actually comes from the Greek, as Mosby's Medical Dictionary, 9th edition. © 2009, Elsevier indicates. The etymology is “Gk, megas, large, karyon, nut, kytos, cell”. The citation is available at http://medical-dictionary.thefreedictionary.com/megakaryocyte. The introduction provides and otherwise very simple and clear explanation. The explanation of platelets and basic roles of MK cells is welcome.

• History: Timeline is very detailed, but completely devoid of citations. There absolutely needs to be citations, preferably at least one citation per entry. The timeline seems very long. It might be advisable to speak with Dr. Hill and see if the information is too excessive.

• Structure: More citations are necessary. The main article used for this section is an old review article from 1964 about rat spleen, so the citations need to include the remark “as reviewed in”, but it also might be a good idea to find more recent relevant claims to support your explanations. “Portions of the intermediate zone showed ribosomes are also present in this layer …” is worded awkwardly. “The vesicular stage in the formation of platelets are found in this area with vesicles of about 400 A showing to be present as they align in rows” is a little unclear in its wording and containing improper grammar. “Further” needs a comma after it in the sentence “Further the alpha granules express the adhesion molecule P-selectin and CD63”. In “This region includes membranes derived from megakaryocytic smooth endoplasmic reticulum which has been organised into a dense tubular system”, it is unclear what noun the phrase “which has been organised into a dense tubular system” is modifying. The membranous zone subsection could benefit from further clarification. More pictures or diagrams depicting the structure would be valuable here.

• Development and Maturation Process: This section is worded very well and, in particular, gave me a clear understanding of the steps in Megakaryocyte development.

• Signaling: The topic heading is misspelled. The phrase “… whereas anti-sense oligodeoxynucleotides of c-mpl inhibits the formation …” should be “… whereas anti-sense oligodeoxynucleotides of c-mpl inhibit the formation …”. Also, “The initial stages of JAK/STAT signalling involves TPO binding to 2 CD110 attached to JAK2s” contains the same type of grammatical and spelling error.

• Function and Role: “Many studies have illustrated that there is a complex interplay between megakaryocytes and bone development” needs to be cited to a reference. The cited pictures and the student authored images are excellent. Double check the citation for Figure 4, as the pictures used to compose the image may require further citation. In general, the section reads smoothly, but requires more citation.

• Pathology: The citations for the references need to be integrated into the text. The grammar of the entire subsection needs to be revised, as it interferes with understanding the highly technical content. The video is a helpful addition to understanding ET.

• The Future of Megakaryocytes: This section is worded well, yet oddly specific for a section serving as the conclusion of the wiki. Again, more citation is required.

3. Are citation and references for the topic appropriate?

• There appeared to be a sparse number of citations where the material presented was not appropriately attributed to the source of the information. In many cases, either information cited from review articles needed to be identified as such or additional citation of information in general was required.

• No citation major citation errors were immediately apparent in the wiki document, but it would be a wise course of action to double check for any errors, as any mistakes would be a serious source of concern. In particular, the latter two images of the section Function and Role, seem to need more in depth citation of the original images used to create the student authored images.

4. Is the wiki instructive to peers by making use of insightful diagrams, tables or figures and/or examples or explanations authored by the group members? • The wiki did present material in a manner friendly and instructive to peers.

• There was a student created image.

• The figures ought to have more creative names than Figure #.

• The figure depicting the histological image of a megakaryocyte helps to give a beautiful picture of the cells being described.

• Some of the really cool media created by students were the banner, clearly depicting megakaryocytes, and summary picture clearly depicting the different biological roles of megakaryocytes.

5. Is it clear that dedicated research has been conducted to connect basic and applied sciences, and does the effort go beyond the formal class material?

• Dedicated research and connections were made between basic and applied sciences, in particular in the sections Pathology and The Future of Megakaryocytes.

• The students displayed considerable effort to go beyond the formal class material to research their topic in depth, however, as mentioned earlier, much of the information used came from review articles not cited as such, or not cited at all.

6. Does the group relate the content of the wiki to the primary learning aims of cell biology?

The primary learning aims of cell biology are to understand the relationship of structure and function within the cell as well as broadly within the tissue and organism.

• The project clearly addresses structure and function within the sections so titled. The section Development and the other sections, particularly Function and Role and Pathology, help to put megakaryocytes in the context of the rest of the body.

7. Final thoughts

Reading this wiki page on Megakaryocytes was certainly informative and helpful toward advancing understanding of them. The structure and delivery of the information appears to be to the appropriate level, if sometimes perhaps more technical than might be expected of this course. The main areas of focus should be the aforementioned citation issues and completing some sections such as Pathology that appear unfinished. In general, however, this appears to be an excellent and informative project. Great work, and good luck with finalising your project in the coming weeks.

Red Blood Cells Group 2 - Peer review by z3465531

1. Are the key points of the topic delineated and clearly presented in detail?

• The key points of the topic were easily identified as aspects of red blood cells according to the title figure, detailed structured subheadings, and a very clear introduction.

• Clear language was used throughout.

• As a result, the article was almost always easy to follow and informative.

2. Are the content, headings and sub-headings, diagrams, tables, graphs appropriate and do they indicate sufficient comprehension of the allocated topic?

• The content presented was detailed, appropriate, and was usually likewise presented, but some sections such as Diseases and Abnormalities and Current Research are perhaps overly specific.

• The figures were appropriate and sufficient in some sections, and they helped convey sufficient comprehension of RBCs. Some sections as mentioned below could benefit from additional figures.

• The title banner, student authored, makes for a great display of the topic, though it seems a relatively minor edit.

• A glossary could be a helpful addition to the wiki page.

• Introduction: The introduction is very clear and straightforward and delivers a very clear description of red blood cells. The introduction touches on all of the aspects of structure, function, synthesis, and disease, previewing the sections to follow, without becoming overly detailed. Be sure to complete the fragment at the end of the section. This has been a common error in other projects, and this cautionary note will not be repeated for the other sections so as not to be too onerous, but be sure to cite review articles, such as reference [1], as “as reviewed in [1]”. Otherwise, this is a brilliant introduction.

• History: Timeline is very detailed and appropriately cited. The timeline seems to be just the right length to give the reader a comprehensive picture of the history of red blood cells without facing excessive details.

• Structure: The section of structure gives a very clear explanation of the specialized structure of red blood cells while even touching briefly on the function and development underlying this structure. More citations, however, are necessary. “ These types of cells lack cell nucleus and some organelles which accounts for its thin biconcave shape.” How? “A suggested reason for this difference in cell diameter is due to the lack of nucleus.” Suggested by whom? Citations here are necessary and would add also legitimacy to the narrative. Fragments such as “Ribosome population decreased to compensate for hemoglobin synthesis” ought to be rewritten into complete ideas. There are several citations in this section which should be converted into numbered links. That said, the material is written very straightforwardly and at an appropriate level of complexity. The electron micrograph of the RBC is great.

• Function: This section is worded very well and gave me a clear understanding of the multiple functions of RBCs without becoming too technical. The way in which many of the citations are delayed until the end of the paragraph is confusing. It would be helpful for the citations to be more closely associated with the specific information that the specific reference contributed. The content here is some of the densest so far in the wiki, so it would be helpful for some cited pictures and the student authored images to assist the reader’s comprehension. In general, the section reads smoothly, but requires more citation.

• Synthesis and Regulation: This section is worded very well and, in particular, gave me a clear understanding of the steps in RBC development. The figure is very instructive for RBC development, but it requires an informative title and description attached to it on the main page. If you are unsure how to do this, be sure to review the relevant section in the introductory labs. There are chunks of text dispersed throughout the text that are missing citations.

• Diseases and Abnormalities: This section and its many subsections contain the clearest most complete citations for the references, and also pay attention in many instances to the importance of citing a review article differently than primary research. The citations are integrated into the text well. The quality of writing in is superb and conveys a sophisticated understanding the highly technical content. The content is perhaps covered in some excessive detail, as it is easily the longest section in the wiki, but it would seem a shame to cut down on such a well-crafted review of the maladies associated with RBCs.

• Current Research: This section gives a well expressed account of a single primary research article, but it seems to be only one example of current research, and described in seemingly excessive detail. A section titled Current Research ought to represent more of the current field of research in RBCs, and give less technical an account of the research. The section also seems like an oddly specific for a section serving as the conclusion of the wiki. A separate brief conclusion and glossary might be beneficial.

3. Are citation and references for the topic appropriate?

• There appeared to be a sparse number of citations where the material presented was not appropriately attributed to the source of the information. In many cases, either information cited from review articles needed to be identified as such or additional citation of information in general was required. The notable exception to this is Diseases and Abnormalities. Team members ought to emulate the research techniques and citations that were employed in the making of this section.

• No major citation errors were immediately apparent in the wiki document, but it would be a wise course of action to double check for any errors, as any mistakes would be a serious source of concern.

4. Is the wiki instructive to peers by making use of insightful diagrams, tables or figures and/or examples or explanations authored by the group members? • The wiki did present material in a manner friendly and instructive to peers.

• There was a student created image, although the edit seemed somewhat minor.

• The figures have appropriate descriptive names, except in the case where there is no descriptive name provided at all.

• The electron micrograph of the red blood cell to gives a beautiful picture of the cells being described.

• It could be useful to look at some of the media created by students in Group 1 including their banner, clearly depicting Megakaryocytes, and summary picture clearly depicting the different biological roles of megakaryocytes.

5. Is it clear that dedicated research has been conducted to connect basic and applied sciences, and does the effort go beyond the formal class material?

• Dedicated research and connections were made between basic and applied sciences, in particular in the section Diseases and Abnormalities. Current Research could use more diverse content.

• The students displayed considerable effort to go beyond the formal class material to research their topic in depth, however, as mentioned earlier, with the exception of the section Diseases and Abnormalities, much of the information used came from review articles not cited as such, or not cited at all.

6. Does the group relate the content of the wiki to the primary learning aims of cell biology?

The primary learning aims of cell biology are to understand the relationship of structure and function within the cell as well as broadly within the tissue and organism.

• The project clearly addresses structure and function within the sections so titled. The section Synthesis and Regulation and the other sections, particularly Diseases and Abnormalities, help to put RBCs in the context of the rest of the body.

7. Final thoughts

Reading this wiki page on RBCs was certainly informative and helpful toward advancing understanding of them. The structure and delivery of the information appears to be to the appropriate level, if sometimes perhaps more technical than might be expected of this course. The main areas of focus should be the aforementioned citation issues and completing some sections such as the Introduction, Structure, and Current Research that appear unfinished to varying degrees. In general, however, this appears to be an excellent and informative project. Great work, and good luck with finalising your project in the coming weeks.

B Lymphocytes Group 3 - Peer review by z3465531

1. Are the key points of the topic delineated and clearly presented in detail?

• The key points of the topic were identified as aspects of B cells according to the title figure, structured subheadings, and a clear introduction.

• Clear language was often used.

• As a result, the article was typically easy to follow and informative.

2. Are the content, headings and sub-headings, diagrams, tables, graphs appropriate and do they indicate sufficient comprehension of the allocated topic?

• The content presented was appropriate, but some sections, as detailed below, were either overly specific or not detailed enough.

• The figures were appropriate, but sparse, and do not appear be have an appropriate reference given each’s details. Some sections as mentioned below could benefit from additional figures.

• The title banner could be student authored if the group is looking for a good figure to edit to make a great display of the topic and fulfil the associated requirement.

• A glossary could be a helpful addition to the wiki page.

• An important note that applies to all sections: There are very few citations throughout the wiki. Large sections of the wiki are have no reference attributed at all. This really needs to be corrected. Citations need to be added. Even if a piece of information seems basic, try to find support for it in a review article. Additionally, a common error in other projects, if using a review article, be sure to cite review articles “as reviewed in [#]”.

• Introduction: The introduction is very clear and straightforward and delivers a very clear description of B-cells. The introduction touches on all of the aspects of structure, function, synthesis, previewing the sections to follow without becoming overly detailed. Be sure to add citations, as there is a lot of specific information included here, and source of the information, at the very least a review article, needs to be credited. Otherwise, this is a brilliant introduction.

• History: Timeline is very short and contains only a single citation. Have nothing of any consequence concerning B cells happened since 1976? Look at the History section presented in Group 2 as a good example for referencing in a timeline and a suitable length. Those events described so far, however, do seem significant.

• Development & Location and Activation: These sections are very technical and difficult to follow well. Cells is misspelt at “cels” in one instance and B cells is not consistently labeled with an upper case B. The citations should be made according to the instructions that were given in lab. The figure is very instructive for B-cell development, but the mechanisms in it appear to be highly technical and illegible at the level to which it has been shrunk. If you are unsure how to do this, be sure to review the relevant section in the introductory labs. There are chunks of text dispersed throughout the text that are missing citations. In particular, the author of this section might want to consider creating subsections to assist the reader in understanding the different possible fates in B cell development. Figures would be very valuable here.

• Types of B-cells: While this section still requires more citations, this section models best the practice of developing useful subheadings for the purpose of organizing the information for better instruction to the reader. This section is very clear and provides a great scaffolding for the reader to organize the dense information and better understand the wiki as a whole. Figures would be very valuable here.

• Structure: The section of structure gives a very detailed technical account of the structure of B cells. While the detail at times appears to be excessive and presented in a difficult way for the reader to understand, the summary and figure are useful tools for learning. Be aware that the figure here does not appear to be appropriately cited displaying the license permitting its reproduction. More citations, however, are necessary. This section is completely devoid of citations, which is not acceptable, as the information presented is very clearly from one or more sources in detail. The table of B-cell surface molecules is really valuable, but needs to be cited. It is informative that this section refers back to a concept in function describing the structure justification behind an earlier mentioned functional activity between B cells and T cells.

• Function: This section is worded well and does a good job of dividing up the concepts of B cell function and giving adequate descriptions without becoming too technical. There are some instances in which the author uses citation appropriately, as in the subsection Initiation of T-cell Immune Response. It would be helpful if elsewhere the citations were likewise more frequent and closely associated with the specific information that the specific reference contributed. The content here is some of the densest so far in the wiki, so it would be helpful for some cited pictures and the student authored images to assist the reader’s comprehension. The picture provided is excellent and should be presented earlier in the section, but again, the picture needs to have the license permissions provided, as they currently are not. It looks like the whole team needs to be really careful about this. In general, the section has helpful divisions, but requires more citation.

• Role in Disease: The subsection for autoimmunity here is incredibly clear and well written. It needs to have citations, as it currently has none. Pay attention in many instances to the importance of citing a review article differently than primary research. The citations are integrated into the text well. The RA section is also well written. The sections still needs to be completed, so be sure to do that in a timely manner, and make sure that citations are included as the information is posted.

• Applications: This is a very short section again missing citations, though it gives a succinct account of monoclonal antibody production. A section titled Current Research ought to represent more of the current applications of B cells. The section also seems like an oddly specific for a section serving as the conclusion of the wiki. A separate brief conclusion and glossary might be beneficial.

3. Are citation and references for the topic appropriate?

• There appeared to be a very sparse number of citations where the material presented was not appropriately attributed to the source of the information. Throughout, either information cited from review articles needed to be identified as such or additional citation of information in general was required.

• All four images appeared to have citation major citation errors immediately apparent in the wiki document. One figure, B-cell development and B-cell subsets, actually contained a copyright notice, but the copyright notice does not appear to expressly state that the figure is allowed to be reproduced. It would be a wise course of action to double check for any errors, as these mistakes would be a serious source of concern.

4. Is the wiki instructive to peers by making use of insightful diagrams, tables or figures and/or examples or explanations authored by the group members? • The wiki did at times present material in a manner friendly and instructive to peers, but as mentioned above, the project would benefit from more figures and student designed learning tools to help organize all of the information.

• There was no student created image.

• The figures seem to have generally appropriate descriptive names.

• The Functions of a B cell gives a beautiful picture of the cells being described and their functions. Be sure to find out if you are actually allowed to use it.

• It could be useful to look at some of the media created by students in Group 1 including their banner, clearly depicting megakaryocytes, and summary picture clearly depicting the different biological roles of megakaryocytes.

5. Is it clear that dedicated research has been conducted to connect basic and applied sciences, and does the effort go beyond the formal class material?

• It seems that some research and connections were made between basic and applied sciences, in particular in the sections Role in Disease and Applications, but the content needs to be expanded and requires careful research with the inclusion of citations.

• The students displayed effort to go beyond the formal class material to research their topic in depth, however, as mentioned earlier, much of the information used came from review articles not cited as such, or not cited at all.

6. Does the group relate the content of the wiki to the primary learning aims of cell biology?

The primary learning aims of cell biology are to understand the relationship of structure and function within the cell as well as broadly within the tissue and organism.

• The project addresses structure and function within the sections so titled. The section Synthesis and Regulation and the other sections, particularly Diseases and Abnormalities, help to put B cells in the context of the rest of the body.

7. Final thoughts

Reading this wiki page on B cells was informative and helpful toward advancing understanding of them. The structure and delivery of the information appears to be to the appropriate level and even, at times, perhaps more technical than might be expected of this course. The main areas of focus should be the aforementioned many citation issues and completing some sections such as the History, Development, Role in Disease, Applications, and Conclusion that appear unfinished to varying degrees. In truth, however, all of the sections require work concerning missing citations, especially the figures, which demand immediate attention. Apart from these issues, however, this appears to be a helpful and informative project concerning B cells. Good work, and keep going strong with finalising your project in the coming weeks.

Natural Killer Cells Group 4 - Peer review by z3465531

1. Are the key points of the topic delineated and clearly presented in detail?

• The key points of the topic were easily identified in Contents with detailed structured subheadings, and a straightforward introduction.

• Clear language was used throughout. At times, however, the vocabulary and technical terms went without full explanation.

• As a result, the article was generally easy to follow and informative.

2. Are the content, headings and sub-headings, diagrams, tables, graphs appropriate and do they indicate sufficient comprehension of the allocated topic?

• The content presented was appropriate and usually likewise presented, but some sections such as Rheumatoid Arthritis in Abnormalities in Disease are perhaps too long and specific.

• The figures were appropriate and sufficient in some sections, and they helped convey sufficient comprehension of NK cells. Some sections from additional figures, but the initial goal should be confirming that the figures are permissible by license so far.

• The title banner, makes for a great display of the topic, though it is not immediately clear that the copyright allows for the reproduction of the image on this wiki. The copyright is “This image is protected under U.S. copyright law. Prior to purchase it can be used for one time, internal comp purposes only.”

• A glossary will be a helpful addition to the wiki page when completed.

• An important note that applies to many sections: Large sections of the wiki are have no citation, or only have a collection of citations at the end of a long paragraph. Citations need to be added within the text. Even if a piece of information seems basic, try to find support for it in a review article. Additionally, a common error in other projects, if using a review article, be sure to cite review articles “as reviewed in [#]”.

• Lymphocytic NK Cells (Introduction): The introduction is very clear and straightforward and delivers a brief description of NK cells. The introduction touches on all of the aspects of function and diseases, but is perhaps light in addressing structure and synthesis, previewing some of the sections to follow, without becoming overly detailed. It might be wise to include previews of the other important sections.

• History: Timeline is relatively short, though appropriately cited. The timeline seems is shorter than some of the other projects because of the relatively recent classification of NK cells, but it might be worthwhile to look for additional important events in the history of NK cells. Look at the History section presented in Group 2 as a good example for referencing in a timeline and a suitable length. Those events described so far, however, do seem significant.

• Structure: The section of structure gives a very clear explanation of the specialized structure of NK cells while even touching briefly on the function and development underlying this structure. More citations, however, are necessary. The histology slide of a large granular lymphocyte is a clear picture, but its relevance to NK cells is not immediately apparent from the text that is given: “Most commonly NK cells are large granular lymphocytes, meaning that have large round nueclei with an absence of a nucleoli surrounded by a large transparent cytoplasm. Granules can be seen throughout the cell giving the cell its name.” Be aware “nueclei” is misspelt. Be sure to finish this section, and be careful of the image that you have uploaded for receptors, unless you have requested and received permission to use this image, it should not be uploaded to your wiki page. That said, the material is otherwise written very straightforwardly and at an appropriate level of complexity.

• Function of NK cells: This section is worded very well and gave me a clear understanding of the multiple functions of NK cells without becoming too technical. The citations, however, need to be more frequent. For example, right away the question “The are 3 main functions of NK cells 1. CD56(dim) in cytokinesis 2. CD56(bright) in cytotoxicity 3. Clonal properties for tumour growth inhibition” needs justification by citation, i.e. How do you know this? And to whom do you owe credit for having this information? The way in which many of the citations are delayed until the end of the paragraph is confusing. It would be helpful for the citations to be more closely associated with the specific information that the specific reference contributed. The content here is some of the densest so far in the wiki, so it would be helpful for some cited pictures and the student authored images to assist the reader’s comprehension. One picture is supplied, but again, the picture does not appear to be allowed to be freely used unless the authors of this wiki have requested and obtained permission from the journal Science. In general, the section reads smoothly, but requires more citation integrated into the text, rather than a single citation at the end.

• Abnormalities in Disease: The summary table at the start of this section is a really helpful tool for originating reading of the subsequent sections to follow. Again, however it is unclear, looking at the original article from which it was obtained, whether or not the table can be used in its entirety verbatim. If you are unsure how to do this, be sure to review the relevant section in the introductory labs. There are chunks of text dispersed throughout the text that are missing citations. Perhaps this is a good opportunity to modify this table for use in your own words so that the requirement of a student created figure is also fulfilled? While this section is worded well and describes in detail different associated diseases, the citations are really insufficient. This section and its many subsections contain the very few citations for the references, in one case only citing one reference for 8 paragraphs of text. Throughout this section, only one reference is cited for each subsection. Pay attention in many instances to the importance of citing a review article differently than primary research. Be sure to use correct citations for the two incomplete subsections when writing them.

• Current Research: This section gives a well expressed accounts of research topics, but they all are cited to articles that are each over ten years old. Nevertheless, the topics described to seem interesting, if somewhat esoteric to the content provided in this course using terminology such as “HLA haplotype-mismatched”. A separate brief conclusion and glossary might also be beneficial.

3. Are citation and references for the topic appropriate?

• While the citations for this group were preferable to some groups which neglected to cite some entire sections, the citations tended to be sparse and citing references for general paragraphs rather than the specific information within the paragraph. In many cases, either information cited from review articles needed to be identified as such or additional citation of information in general was required.

• It is only readily apparent that the figure Histology slide of large granular lymphocyte is permitted to be freely reproduced, a figure which needs further clarification of its relevance specifically to NK cells. It would be advisable for the group to confirm that the other citations do not contain any licensing errors, as any mistakes would be a serious source of concern.

4. Is the wiki instructive to peers by making use of insightful diagrams, tables or figures and/or examples or explanations authored by the group members? • The wiki did present material in a manner friendly and instructive to peers.

• There was no student created image.

• The figures have appropriate descriptive names.

• The table summarizing NK Cells in Disease is extremely helpful in describing the key applications of NK Cells in disease conditions and is a valuable part of the project, provided that it is allowed to be used.

• It could be useful to look at some of the media created by students in Group 1 including their banner, clearly depicting Megakaryocytes, and summary picture clearly depicting the different biological roles of megakaryocytes.

5. Is it clear that dedicated research has been conducted to connect basic and applied sciences, and does the effort go beyond the formal class material?

• Research and connections were made between basic and applied sciences, in particular in the section Abnormalities in Disease and Current Research sections, but the quality of that research is called into questions to be paucity of different resources cited in each particular subsection.

• The students displayed considerable effort to go beyond the formal class material to research their topic in depth, however much of the information used came from review articles not cited as such, and the subsections would benefit from a synthesis of research from multiple different sources, whereas most paragraphs in this paper have only a single citation.

6. Does the group relate the content of the wiki to the primary learning aims of cell biology?

The primary learning aims of cell biology are to understand the relationship of structure and function within the cell as well as broadly within the tissue and organism.

• The project clearly addresses structure and function within the sections so titled. The other more applied sections help put NK cells in the context of the rest of the body, but the wiki seems to be missing a section on development to give further context of NK cells within the tissue and organism as a whole.

7. Final thoughts

Reading this wiki page on NK cells was informative and helpful toward advancing understanding of them. The structure and delivery of the information appears to be to the appropriate level, if at times perhaps more technical than might be expected of this course. The main areas of focus should be the aforementioned citation issues and completing some sections such as the Structure, Abnormalities in Disease, and possibly adding the sections Development, Conclusion, and Glossary. In general, however, this appears to be an instructive and informative project. Good work, and best of fortune with finalising your project in the coming weeks.

Mast Cells Group 5 - Peer review by z3465531

1. Are the key points of the topic delineated and clearly presented in detail?

• The key points of the topic were very easily identified as aspects of mast cells according to the title figure, detailed structured subheadings, and a very clear introduction.

• Extremely clear language was used throughout. Reading this article was fun and enjoyable as well as informative.

• As a result, the article was almost always easy to follow and informative.

2. Are the content, headings and sub-headings, diagrams, tables, graphs appropriate and do they indicate sufficient comprehension of the allocated topic?

• The content presented was always detailed, appropriate, and was usually likewise presented. The authors clearly knew the topic very well.

• The figures were very appropriate and excellent in almost all sections, and they helped convey sufficient comprehension of mast cells. Mentioned below, however, are a few cautionary notes to keep in mind when revising.

• The title banner, student authored, makes for a great display of the topic, though it seems a relatively minor edit. The authors should be aware that the picture is missing the addendum that the picture is being used a part of a student project. Look to the labs for a detailed account of the information that needs to be included with each picture added to the wiki.

• A glossary is currently short, but a helpful addition to the wiki page.

• This has been a common error in other projects, and this cautionary note will not be repeated for the other sections so as not to be too onerous, but be sure to cite review articles, such as reference [1], as “as reviewed in [1]”.

• Introduction: The introduction is very clear and straightforward and delivers a very clear description of mast cell. The introduction touches on all of the aspects of structure, function, development, and disease, previewing the sections to follow, without becoming overly detailed. In general, this is a brilliant introduction. The way in which the introduction does not assume that the reader already knows the biochemical and functional significance of immunological structures such as granulocytes is very friendly to the reader. It is helpful that the citations are throughout.

• History: Timeline is very detailed and appropriately cited. The timeline seems to be just the right length to give the reader a comprehensive picture of the history of mast cells without presenting excessive details.

• Morphology: The section of morphology gives a very clear explanation of the specialized structure of mast cells while even touching briefly on the function and development underlying this structure. The picture, which is appropriately referenced, gives a truly rewarding depiction of secretory granules. The accompanying information is clearly stated at an appropriate level for this course, supplying easily understood, yet valuable information such as “Mast cells possess other organelles such as mitochondria, golgi [sic] apparatus, endoplasmic reticulum, and ribosomes”. The picture is appropriately mentioned in the text. The only critique that ought to be made is that the excellent figure should be given an original name.

• Function: This section is worded very well and gave me a clear understanding of the multiple functions of mast cells without becoming too technical. In fact, the author of this section clearly made an effort to understand the complex content and make it more accessible. The citations remain integrated. The only suggestion that comes to mind is that the author tries splitting this section into further subsections of Immune System and Wound Healing/Tissue repair. This is another excellently composed section.

• Origin and Migration, Differentiation, Activation, Mediators, and Degranulation: These sections are worded very well and, in particular, gave me a clear understanding of the steps in mast cell developmental origin and migration and beyond. The first figure is very instructive for showing where mast cells fit in immune cell development, as well as detailing some of the techniques used to better understand mast cell development. The only thing that really ought to be kept in mind with this section is that the figures provided requires an informative title, and the content provided when clicking on the picture should be revised. If you are unsure how to do this, be sure to review the relevant section in the introductory labs. The student authored table and following Mast cell signaling pathway picture are very helpful in describing otherwise complicated genetics and cellular signaling pathways in simple terms. The linked video to mast cell degranulation is a beautiful and informative resource.

• Pathology: This section and its many subsections contain clear complete citations for the references. The citations are integrated into the text well, but are missing from some of the content in the tables. The quality of writing in is superb and conveys a sophisticated understanding the highly technical content. The content is perhaps covered in some excessive detail, as it is easily the longest section in the wiki, and seems to be about as long as or longer than the other sections combined. It would seem a shame to cut down on such a well-crafted review of the maladies associated with Mast cells. In particular, the figures and tables are well distributed throughout the otherwise confusing text and are very beneficial to the comprehension of such text. I would remark one cautionary comment that, for figures such as Mast Cell Role in Asthma, it appears that the copyright notice does not grant you full access to reusing the diagram on this wiki unless you have obtained separate permission: “All of the material available from the PMC site is provided by the respective publishers or authors. Almost all of it is protected by U.S. and/or foreign copyright laws, even though PMC provides free access to it. (See Public Domain Material below, for one exception.) The respective copyright holders retain rights for reproduction, redistribution and reuse. Users of PMC are directly and solely responsible for compliance with copyright restrictions and are expected to adhere to the terms and conditions defined by the copyright holder. Transmission, reproduction, or reuse of protected material, beyond that allowed by the fair use principles of the copyright laws, requires the written permission of the copyright owners. U.S. fair use guidelines are available from the Copyright Office at the Library of Congress.” This section is comprehensive and detailed.

• Further Notes about sections: It might be unnecessary to include a section for Current Research as such as been integrated throughout the wiki already. Still, as the final section on Pathogenesis is so expansive and detailed, it might be wise to include a separate brief conclusion to bring the reader back to the crucial points of mast cells.

3. Are citation and references for the topic appropriate?

• The citations were plentiful and comprehensive, with only minor exceptions mentioned above. It is worth reminding, however, that information cited from review articles needs to be identified as such.

• No major citation errors were immediately apparent in the wiki document, but it would be a wise course of action to double check for any errors, as any mistakes would be a serious source of concern. This is especially relevant for the occasional instances mentioned in which the copyright permissions of the images is questionable.

4. Is the wiki instructive to peers by making use of insightful diagrams, tables or figures and/or examples or explanations authored by the group members? • The wiki did present material in a very friendly and instructive manner to peers.

• There were multiple student created images, and they were very effective in clarifying the otherwise complicated associated concepts.

• The figures have appropriate descriptive names, except in the aforementioned cases where there are no descriptive names provided at all.

• There were multiple excellent pictures and figures provided.

5. Is it clear that dedicated research has been conducted to connect basic and applied sciences, and does the effort go beyond the formal class material?

• Dedicated research and connections were made throughout the project. Well done.

• The students displayed considerable effort to go beyond the formal class material to research their topic in depth. In the case of pathology, this research is perhaps too extensive and lengthy, but it was nevertheless clearly conveyed and valuable reading.

6. Does the group relate the content of the wiki to the primary learning aims of cell biology?

The primary learning aims of cell biology are to understand the relationship of structure and function within the cell as well as broadly within the tissue and organism.

• The project clearly addresses structure and function within the section physiology. The parts of the section physiology pertaining to activity within the tissue and the section of pathology helped to put mast cells in the context of the rest of the body.

7. Final thoughts

Reading this wiki page on mast cells was incredibly informative and helpful toward advancing understanding of them. The structure and delivery of the information extremely well worded and presented at an accessible level, nonetheless rich in scientific content. The main areas of focus should be a Conclusion section and expanded Glossary, but in truth there is very little criticism to offer. Double check those figure copyright permissions. At the end of the day, this just simply appears to be a top notch project. Incredible work, and good luck with finalising your project in the coming weeks.

Eosinophils Group 7 - Peer review by z3465531

1. Are the key points of the topic delineated and clearly presented in detail?

• The key points of the topic were very easily identified as aspects of eosinophils according to the title figure, detailed structured subheadings, and a very clear introduction.

• Extremely clear language was used throughout. Reading this article was fun and enjoyable as well as informative.

• As a result, the article was almost always easy to follow.

2. Are the content, headings and sub-headings, diagrams, tables, graphs appropriate and do they indicate sufficient comprehension of the allocated topic?

• In some cases that will be listed below, it seemed that necessary sections for the project were omitted. In all sections that were addressed, however, content presented was always detailed, appropriate, and was usually likewise presented. The authors clearly knew the topic very well.

• The figures were very appropriate and excellent in all sections, and they helped convey sufficient comprehension of eosinophils.

• The title banner makes for a great display of the topic.

• A glossary is not yet included and might make for a helpful addition to the wiki page.

• This has been a common error in other projects, and this cautionary note will not be repeated for the other sections so as not to be too onerous, but be sure to cite review articles, such as reference [1], as “as reviewed in [1]”.

• Introduction: The project probably ought to have an introduction describing the fundamental characteristics of eosinophils.

• History: The timeline is relatively short and only cites two different references. Furthermore, the timeline only seems to outline a 300 year build up to the first usage of the term eosinophil. There is a case to be made that the most important aspects of eosinophils four our purposes are all of the history of discoveries about eosinophils since 1879. Some more modern discoveries about eosinophils probably ought to be included and appropriately cited.

• Birth, Life, and Death in the Body: These sections are worded very well and, in particular, gave me a clear understanding of the steps in eosinophils cell developmental origin and migration and ultimate death. The first figure is very instructive for showing where eosinophils fit in immune cell development, and the other figure depicts eosinophils excellently demonstrating their granular nature and even presenting a neutrophil in contrast. The only thing that really ought to be kept in mind with this section is that the first figure provided requires an informative title, and the picture should be provided with an explanatory note. If you are unsure how to do this, be sure to review the relevant section in the introductory labs.

• Structure: The section of morphology gives a very clear explanation of the specialized structure of eosinophils taking the time to describe granules clearly and in detail. The multiple pictures, which are appropriate have an appropriate associated copyright notice, gives a truly rewarding depiction of eosinophil morphology, clearly showing the bi-lobed nucleus and secretory granules. The accompanying information is clearly stated at an appropriate level for this course, supplying easily understood, yet valuable information. The text is closely related to the pictures provided, but it might be helpful for the authors to specifically refer to the accompanying pictures in the text of the wiki. The Characteristics of Eosinophils figure should have a brief description of its relevance. This was a clear and superb section, and a joy to read.

• Function: Function is mentioned briefly in the last paragraph of Birth, Life, and Death in the Body, but it seems like Function is important enough to merit its own section. It might be worthwhile to create this section in the revision of the project.

• Pathology (Role in Allergy and Diseases): This section and its many subsections contain clear complete citations for the references. The citations are integrated into the text very well. The quality of writing in is superb and conveys a sophisticated understanding the highly technical content. In particular, the figures, pictures, and tables are well distributed throughout the otherwise confusing text and are very beneficial to clarifying the text. Particularly impressive is the proactive way in which permission has been obtained for those pictures and diagrams which require formal requests for permission for use. They are very clear pictures that clearly describe how eosinophils can play a role in allergy and disease. This section is comprehensive and detailed, and importantly, not over detailed so as to flood the student with a deluge of information. The well-chosen figures appear to assist in keeping the descriptions succinct.

• Further Notes about sections: It might be wise to include a separate brief conclusion to bring the reader back to the crucial points of mast cells as well as a glossary of terms used.

3. Are citation and references for the topic appropriate?

• The citations were plentiful and comprehensive, with only minor exceptions mentioned above. It is worth reminding, however, that information cited from review articles needs to be identified as such.

• No major citation errors were immediately apparent in the wiki document, but it would be a wise course of action to double check for any errors, as any mistakes would be a serious source of concern. This wiki page in particular is an exemplary job with copyright permissions.

4. Is the wiki instructive to peers by making use of insightful diagrams, tables or figures and/or examples or explanations authored by the group members? • The wiki did present material in a very friendly and instructive manner to peers.

• There were no student created images.

• The figures have appropriate descriptive names and explanations, except in the aforementioned cases where there are no descriptive names provided at all.

• There were multiple excellent pictures and figures provided.

5. Is it clear that dedicated research has been conducted to connect basic and applied sciences, and does the effort go beyond the formal class material?

• Dedicated research and connections were made throughout the project. Well done.

• The students displayed considerable effort to go beyond the formal class material to research their topic in depth in the particular sections that were addressed. The authors of this wiki should seriously consider addressing the omitted sections mentioned above.

6. Does the group relate the content of the wiki to the primary learning aims of cell biology?

The primary learning aims of cell biology are to understand the relationship of structure and function within the cell as well as broadly within the tissue and organism.

• The project clearly addresses structure and very briefly the function within the section Birth, Life and Death in the Body. The description of an eosinophils development and role in allergy and disease helped to put eosinophils in the context of the rest of the body.

7. Final thoughts

Reading this wiki page on eosinophils was incredibly informative and helpful toward advancing understanding of them. The decision to describe Birth, Life, and Death of eosinophils worked well and was presented at an accessible level, nonetheless rich in scientific content, but development and function might merit their own sections as you work to revise and perhaps expand your wiki in this area. The main areas of focus should first be an Introduction section, revised History section, Conclusion section and Glossary, but in truth there is very little criticism to offer. At the end of the day, this just simply appears to be a top notch project, even if seemingly incomplete. Incredible work, and good luck with finalising your project in the coming weeks.

References