User:Z3465108

From CellBiology

Welcome to Cell Biology 2017!

Lab 1 Assessment

Lab 2 Assessment

  1. Identify a chemical SDS and the risks and hazards of that chemical in text. Add a link to the original SDS
  2. Select 4 reference papers papers related to your selected group project topic sub-section. Read the research papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page.

Lab 3 Assessment - Endo/Exo worksheet questions.

Lab 4 Assessment

  1. Identify a cytoskeletal antibody.
  2. Identify the species deriving the antibody.
  3. Identify the working concentration for the antibody.
  4. Identify a secondary antibody that could be used with this antibody.
  5. Identify a paper that has used this antibody.

This assessment will be due by the next lab (Lab 5).

Lab 7 Assessment

The following peer assessment exercise should be completed before next lab (Lab 8 - 2 May) as your individual assessment for this week (lab missed due to public holiday).

Your answer should be pasted in 2 places

  1. onto each project discussion page (Note you should add anonymously to the discussion page)
  2. your own individual student page for my assessment.

Each individual will provide a brief assessment of the other groups projects. This should take the form of a brief critical (balanced) assessment identifying both the positive (good) and negative (bad) aspects of the project page as it currently exists online.

You may if you choose, use the final project assessment criteria as a guide. Though you are also welcome to use your own criteria.

Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.

Attendance

Z3465108 (talk) 16:04, 7 March 2017 (AEDT)

Z3465108 (talk) 16:51, 21 March 2017 (AEDT)

Z3465108 (talk) 16:00, 28 March 2017 (AEDT)

Z3465108 (talk) 15:05, 4 April 2017 (AEST)

Z3465108 (talk) 15:10, 9 May 2017 (AEST)

Z3465108 (talk) 15:04, 16 May 2017 (AEST)

Z3465108 (talk) 15:06, 23 May 2017 (AEST)

Individual Assessments

Lab 1

Sec4p induced endocytosis.jpg

Sec4p overrides the inhibition of cortical actin polymerization to induce compensatory endocytosis

Jesper Johansen, Gabriel Alfaro, Christopher T Beh Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization. PLoS Biol.: 2016, 14(8);e1002534 PubMed 27526190

© 2016 Johansen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

(5/5)

Lab 2

Part 1

Hydrogen peroxide


Risks/Hazards:

  • skin corrosion/ irritation
  • serious eye damage/ eye irritation


Hydrogen peroxide SDS


Part 2

Cell-matrix Interactions

Harini Chakravarthy, Xueying Gu, Martin Enge, Xiaoqing Dai, Yong Wang, Nicolas Damond, Carolina Downie, Kathy Liu, Jing Wang, Yuan Xing, Simona Chera, Fabrizio Thorel, Stephen Quake, Jose Oberholzer, Patrick E MacDonald, Pedro L Herrera, Seung K Kim Converting Adult Pancreatic Islet α Cells into β Cells by Targeting Both Dnmt1 and Arx. Cell Metab.: 2017, 25(3);622-634 PubMed 28215845

Pancreatic alpha cells can be converted into beta cells in an environment of significant beta cell loss. The experiment showed that when alpha cell regulators, Aristaless-related homeobox (Arx) and DNA methyltransferase 1 (Dnmt1), were inactivated, there as efficient conversion of alpha cells into beta cells. This shows a close relationship with alpha and beta cells.

Joana Almaça, Judith Molina, Danusa Menegaz, Alexey N Pronin, Alejandro Tamayo, Vladlen Slepak, Per-Olof Berggren, Alejandro Caicedo Human Beta Cells Produce and Release Serotonin to Inhibit Glucagon Secretion from Alpha Cells. Cell Rep: 2016, 17(12);3281-3291 PubMed 28009296

In non-diabetic individuals, the release of serotonin from pancreatic beta cells, stimulated by increases in glucose, decrease cAMP in nearby alpha cells, inhibiting glucagon secretion. This interaction between alpha and beta cells shows the importance of an intricate system of pancreatic cells.


(9/10)

Lab 4

Alpha tubulin monoclonal antibody

  • Species raised: mouse/IgG1, kappa
  • Working concentration: 0.2mg/ml
  • Used in a Western Blot:

Stephanie L Maiden, Yuliya I Petrova, Barry M Gumbiner Microtubules Inhibit E-Cadherin Adhesive Activity by Maintaining Phosphorylated p120-Catenin in a Colon Carcinoma Cell Model. PLoS ONE: 2016, 11(2);e0148574 PubMed 26845024


(5/5)

Lab 5

Comparison of morphology.jpg


Mark Hill (talk) 20:51, 24 April 2017 (AEST) This lab assessment will be marked by the guest presenter and the mark added here when I have received.


Lab 7

Peer reviews

Group 1

The overall layout and format of the page is structured well, with headings and subheadings relevant to the topic area. It shows a comprehensive understanding into the areas that need to be researched in relation to delta cells. The information provided is relevant and shows extensive research. The use of videos and tables is a good mode of interactive learning and is engaging. The background section is a good start to the page, however at the moment is very brief is describing the background to delta cells. The majority of the content in this section discusses the proportion of islet cells, which perhaps should be discussed in another section. Consider looking at the background section instead as an introduction which would cover background as well as a brief look into delta cells. The development and structure section seems to still be coming along, and the content so far is good, particularly the comparison with other islet cells. Also, I like how connections have been made to other sections. The function section is quite comprehensive however the format of the content could be enhanced with the addition of more diagrams, the same applies for the cell-matrix interactions section. The pathology section has been covered well, and whilst it hasn't yet been completed, I can see by the layout as to what information is to come. The inclusion of a glossary at the end of the page is an excellent idea and is perfect to accommodate learning, however this should be used more, I noticed that in quite a few sections there are abbreviations with their explanation, consider taking out the explanation and including the abbreviation in the glossary. Overall a good job so far.

Group 2

The page is well set out with appropriate headings and subheadings. Each section is well researched. The introduction is well thought out, however consider expanding on the relation between the pancreas and duct cells, for example, talk about Islets of Langerhans. Whilst the content is comprehensive, there are only two diagrams on the page, it would be useful in understanding the text with more diagrams, videos, or tables, particularly for the function section, perhaps it would be easier to understand with a flow chart of some sort. The pathology/abnormalities section is incomplete, but with the references for articles it looks like it will be covered well. There is yet to be anything included in the glossary. It would be a good idea to use the tool as it would make it easier when using the page. Overall there seems to be a comprehensive understanding of duct cells with the various sections researched well, however the layout could be improved to allow for a more engaging learning experience.

Group 3

The introduction is well thought out and presented. It extensively covers the background of beta cells. Whilst the inclusion of a picture is good, it would be helpful to have it labelled to understand what is shown. The history section is formatted well, the use of a table structures the events in an easily understandable layout. The structure section has been researched extensively, which is shown by the amount of content available. Again, the addition of a photo assists with understanding the text, however, if it were labelled it would be easier to understand what is what, or if not labelled, perhaps referring to the image within the text. A comparison between mice and human structure is covered well. Function, development and signalling, whilst not completed, shows that it is known what is to be included, and this covers the section. The role in disease has been described extensively and the use of a video is engaging. This section could include more about current research, or even lead into a s section on current research. The glossary is a useful tool, and I have noticed that a note has been made it alphabetise this section which is good. Perhaps consider even putting it into a table format to structure the section better. From what is completed so far, there seems to be a good understanding of beta cells.


(very good (8/10)

Tutorial

Student Image Template

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

Referencing

https://www.ncbi.nlm.nih.gov/pubmed

PubMed

Search Plasma Membrane

  • No link - PMID: 28257417
  • Link - PMID 28257417

Javier U Chicote, Rob DeSalle, Antonio García-España Phosphotyrosine phosphatase R3 receptors: Origin, evolution and structural diversification. PLoS ONE: 2017, 12(3);e0172887 PubMed 28257417


Image

Human R3 RPTP members.png

Domain architecture of human R3 RPTP members

2017 Course Content

Moodle

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2017 Revision

2017 Laboratories: Introduction to Lab | Fixation and Staining |


2017 Projects: Group 1 - Delta | Group 2 - Duct | Group 3 - Beta | Group 4 - Alpha

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G