User:Z3429175

From CellBiology

Welcome to Cell Biology 2017!

Lab 1 Assessment

Lab 2 Assessment

  1. Identify a chemical SDS and the risks and hazards of that chemical in text. Add a link to the original SDS
  2. Select 4 reference papers papers related to your selected group project topic sub-section. Read the research papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page.

Lab 3 Assessment - Endo/Exo worksheet questions.

Lab 4 Assessment

  1. Identify a cytoskeletal antibody.
  2. Identify the species deriving the antibody.
  3. Identify the working concentration for the antibody.
  4. Identify a secondary antibody that could be used with this antibody.
  5. Identify a paper that has used this antibody.

This assessment will be due by the next lab (Lab 5).

Lab 7 Assessment

The following peer assessment exercise should be completed before next lab (Lab 8 - 2 May) as your individual assessment for this week (lab missed due to public holiday).

Your answer should be pasted in 2 places

  1. onto each project discussion page (Note you should add anonymously to the discussion page)
  2. your own individual student page for my assessment.

Each individual will provide a brief assessment of the other groups projects. This should take the form of a brief critical (balanced) assessment identifying both the positive (good) and negative (bad) aspects of the project page as it currently exists online.

You may if you choose, use the final project assessment criteria as a guide. Though you are also welcome to use your own criteria.

Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.

Individual Assessment

Lab 7

Group 2 (Ductal Cells)

The content of the ‘Function’ and ‘Structure’ of ductal cells are good. However, I think it could be elaborated further by explaining the Inositol pathway. It can be seen that good research has been done on the ‘History’ part. It could be better by organizing the information in a table to make it easier to follow, as it can be a bit confusing. I believe that the referencing will be done on the ‘History’ and ‘Development’ sections during final touch of the project. Good content for the ‘Development’ sub-heading, although it could be improved by explaining further in details of certain terms or factors that are not familiar such as HNFiB, HNF6 and SOX9 for better understanding of the viewers. Unfortunately, at this point there is no current research found by the team. Overall, the project has really good contents in which it can definitely be enhanced by adding videos or images that are related to the content for viewers to develop deeper understanding.

Group 3 (Beta Cells)

Everything is very well organized. A reasonable amount of research has been done on the ‘Structure’ subheading considering that ample information has been provided including the similarities and differences of the structures both in humans and mice. A good diagram has also been included to explain beta cell destruction. However, I noticed there are some sections done without referencing them. Other than that, the first image on the page does not have proper copyright information. I believe that copyright information has to be completed when the image is being uploaded. The contents of each section are clear except for ‘Function’, ‘Development’ and ‘Signalling’ which seem to be lacking content. However, a brief list has been done under each subheading, which indicates a great start towards the content. It is really good that the treatments mentioned include both human tissue and also embryonic pig tissue. It indicates that the option of treatments is not limited to only human tissue. This group definitely has put in effort considering the amount of information provided on the page. Overall, relevant contents on the page, good flow from one subheading to another subheading therefore it is very easy to follow.

Group 4 (Alpha Cells)

According to the current state of the page, this group certainly needs to put in more effort in collecting all the information together since all the important notes have been listed down in dot points. I am very certain that once everything is completed, it would be a good final project. The images added are in fact very useful in providing better explanations to the viewers. The videos added to the page are in fact very interesting and easy to understand. However, I would suggest that the size of the image under ‘Disease’ subheading to be enlarged just a little bit more. Most of the images have not been referenced properly and there is no copyright information as well. The ‘Current Research’ subheading clearly needs some attention, as it is empty. The subheadings could also be more organized I believe. Overall, it is a good start since a considerable amount of information has been provided.


(8/10)

Lab 5

Tm4control.png


Mark Hill (talk) 20:51, 24 April 2017 (AEST) This lab assessment will be marked by the guest presenter and the mark added here when I have received.


Lab 4

Primary Antibody

Smooth Muscle Actin Antibody

Species raised: Rabit/ IgG Class: Polyclonal

Secondary Antibody

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Research Paper:

Christina C Westhoff, Katharina Schoner, Sylvia Hartmann, Andreas M Sesterhenn, Roland Moll Actin isoform expression patterns in adult extracardiac and cardiac rhabdomyomas indicate a different cell of origin. Virchows Arch.: 2017, 470(3);285-290 PubMed 28091730


Lab 2

Iodic Acid

GHS Classification:

Oxidizing solids (Category 2)

Skin corrosion (Category 1)

Serious eye damage (Category 1)

Hazard statements:

H272 May intensify fire, oxidiser

H314 Causes severe skin burns and eye damage

Further harzards identification can be found here:

Iodic Acid

Group Project Team 1

  • 1

Inchul Lee Human pancreatic islets develop through fusion of distinct β and α/δ islets. Dev. Growth Differ.: 2016, 58(8);635-640 PubMed 27530443


Human pancreatic islets represents distinctive fomation in which α and δ cells are particularly found at the area of peripheral and perivascular. Fetal islets establish first in two distinct types comprise of β or α/δ cells, correspondingly.The α/δ islets turn out to perform a primary function for the progression, in the direction towards the fusion partner dynamically. This article touches on the development of delta cells.

  • 2

A Apelqvist, H Li, L Sommer, P Beatus, D J Anderson, T Honjo, M Hrabe de Angelis, U Lendahl, H Edlund Notch signalling controls pancreatic cell differentiation. Nature: 1999, 400(6747);877-81 PubMed 10476967


The pancreas comprises of exocrine and endocrine cells. However, the molecular techniques regulating the differentiation of these cell types are generally undetermined. Pancreatic endocrine cells share quite a few molecular aspects with neurons and differentiating endocrine cells in the pancreas are found to be scattered within progenitor cells. This illustrates that they may be produced by lateral arrangement through Notch signalling. This paper explains further in details about the signalling of the cells in the developing pancreas.

  • 3

R Jain, E Lammert Cell-cell interactions in the endocrine pancreas. Diabetes Obes Metab: 2009, 11 Suppl 4;159-67 PubMed 19817798


The islets of Langerhans creating the endocrine pancreas are made up of alpha, beta, delta, epsilon and PP cells. Communications between these cells are needed for adjustment of glucose homeostasis of the body. This review argues on how islet hormones, released molecules and ions affects the endocrine cells and in addition to that, how cell adhesion molecules as well as extracellular matrix proteins, modify the function of pancreatic islet. This review article provides information on the cell matrix interactions.

  • 4

Michael R DiGruccio, Alex M Mawla, Cynthia J Donaldson, Glyn M Noguchi, Joan Vaughan, Christopher Cowing-Zitron, Talitha van der Meulen, Mark O Huising Comprehensive alpha, beta and delta cell transcriptomes reveal that ghrelin selectively activates delta cells and promotes somatostatin release from pancreatic islets. Mol Metab: 2016, 5(7);449-58 PubMed 27408771



The researcher detected that the ghrelin receptor is expressed particularly by delta cells within the islet, which was firmly established by fluorescent in situ hybridization and qPCR. Ghrelin engages delta cells in order to stimulate local inhibitory feedback that reduces insulin release instead of engaging to delta cells directly. This paper explains the activation of delta cells by ghrelin.


(10/10)

Lab 1

MET.png

Figure A shows a schematic relationship between gene amplification and polysomy. The proportion of the copy number of the focused gene in comparison for the centromeric section of the chromosome differentiates a multiplication of the copy number of the focused gene deducible to gene amplification as a result of additional copies of the chromosome. Figure B shows FISH evaluation of a gastric cancer cell line or known as HSC58.


Mark Hill (talk) 12:15, 28 March 2017 (AEDT) All good with the image upload and source information on your page. Note that you need a better file name than just "MET" and only needed to add the image a title and the reference to your page (as below) the additional information must go with the image file. Please have a look at the file and I have added the information correctly for you. (4/5)

Schematic relationship between gene amplification and polysomy.[1]

Reference

Hisato Kawakami, Isamu Okamoto, Wataru Okamoto, Junko Tanizaki, Kazuhiko Nakagawa, Kazuto Nishio Targeting MET Amplification as a New Oncogenic Driver. Cancers (Basel): 2014, 6(3);1540-52 PubMed 25055117


Copyright

© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

Attendance

Z3429175 (talk) 16:05, 7 March 2017 (AEDT)

Z3429175 (talk) 15:37, 14 March 2017 (AEDT)

Z3429175 (talk) 17:00, 21 March 2017 (AEDT)

Z3429175 (talk) 16:28, 28 March 2017 (AEDT)

Z3429175 (talk) 18:41, 4 April 2017 (AEST)

Z3429175 (talk) 15:00, 2 May 2017 (AEST)

Z3429175 (talk) 15:19, 16 May 2017 (AEST)

Tutorial

Student Image Template

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

Referencing

https://www.ncbi.nlm.nih.gov/pubmed

PubMed

Search Plasma Membrane

  • No link - PMID: 28260076
  • Link - PMID 28260076

Xijing He, Liying Fan, Zhongheng Wu, Jiaxuan He, Bin Cheng Gene expression profiles reveal key pathways and genes associated with neuropathic pain in patients with spinal cord injury. Mol Med Rep: 2017; PubMed 28260076


Image

Human R3 RPTP members.png

Reference

Javier U Chicote, Rob DeSalle, Antonio García-España Phosphotyrosine phosphatase R3 receptors: Origin, evolution and structural diversification. PLoS ONE: 2017, 12(3);e0172887 PubMed 28257417


Copyright

Copyright: © 2017 Chicote et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


2017 Course Content

Moodle

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2017 Revision

2017 Laboratories: Introduction to Lab | Fixation and Staining |


2017 Projects: Group 1 - Delta | Group 2 - Duct | Group 3 - Beta | Group 4 - Alpha

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G
  1. Hisato Kawakami, Isamu Okamoto, Wataru Okamoto, Junko Tanizaki, Kazuhiko Nakagawa, Kazuto Nishio Targeting MET Amplification as a New Oncogenic Driver. Cancers (Basel): 2014, 6(3);1540-52 PubMed 25055117