User:Z3423497

From CellBiology
Group Projects
This year's main topic is Blood Cell Biology. Each group should discuss with group members the specific sub-topic that will be covered by their project.

Here is a list of some of the cell types (Structure and Function)

Cell Type (PuMed citations)


Below are the groups to which students have been randomly assigned. You should now on the project discussion page add your own suggestion for a specific topic. Once your group has agreed on the topic, add this as a heading to the project page before Lab 3.


2016 Projects: Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7

Group 1: User:Z5017493 | User:Z3330991 | User:Z5020043 | User:Z5020175 | User:Z3489355

Group 2: User:Z5018320 | User:Z5015980 | User:Z3376375 | User:Z3461106

Group 3: User:Z5019595 | User:Z5019962 | User:Z5018925 | User:Z3461911

Group 4: User:Z5020356 | User:Z3463895 | User:Z3376502 | User:Z3423497 | User:Z5021149

Group 5: User:Z5015719 | User:Z3462124 | User:Z3463953 | User:Z5017292

Group 6: User:Z5018866 | User:Z3329177 | User:Z3465531 | User:Z5105710

Group 7: User:Z5021060 | User:Z5016365 | User:Z5016784 | User:Z3414546 | User:Z3417773

Group Assessment Criteria

Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.
Individual Lab Assessments
Lab 8 Assessment
2016 Lab 8 - Lab 8 Assessment (to be completed before Lab 9)
  1. Add your peer assessment to your own student page to the site.
  2. Add your peer assessment to each project discussion page to the site.
Lab 6 Assessment
2016 Lab 6 -
  1. Identify an antibody against your group blood cell protein that is commercially available.
  2. Add a link to the original data sheet page and identify the type of group blood cell protein.
  3. Include the following information: type of antibody (polyclonal, monoclonal), species raised in, species reacts against, types of application uses, and if available any reference using that antibody.
Lab 2 Assessment
2016 Lab 2 - Super resolution microscopy
  1. Find a recent research article (not review) that uses super resolution microscopy technique.
  2. Write a brief summary of the paper (referenced) and what the super resolution microscopy technique showed.
    1. This should not simply be the abstract of the paper.
    2. This can be 2-3 paragraphs no longer.
  3. Include a super resolution microscopy image from the paper.
    1. Therefore the paper must be from a source that you can reuse.
    2. Image uploaded as in Lab 1 (summary box - description/reference/copyright/student image)
    3. Image should appear as a "thumbnail" (thumb) next to your paper summary (with citation legend) See Test page
Lab 1 Assessment
2016 Lab 1 - Lab 1 Assessment (to be completed before Lab 2) The test page I set up in the Lab
  1. Add your own student page to the site.
  2. Add your signature for Lab attendance.
  3. Add a sub-heading.
  4. Add an external Link.
  5. Add an internal Link.
  6. Add an image from PubMed, PloS or BioMed Central journal related to prokaryote cellular component. Make sure it includes both the reference and copyright information, with the file and where it appears on your page.

Attendance

Z3423497 (talk) 11:54, 10 March 2016 (AEDT)

Z3423497 (talk) 11:06, 17 March 2016 (AEDT)

Z3423497 (talk) 11:07, 24 March 2016 (AEDT)

Z3423497 (talk) 10:55, 7 April 2016 (AEST)

Z3423497 (talk) 11:14, 21 April 2016 (AEST)

Z3423497 (talk) 11:02, 5 May 2016 (AEST)

Z3423497 (talk) 11:08, 12 May 2016 (AEST)


  • Z8600021 You have attended only 7 practical classes.

Lab 1 Assessment

Search Pubmed

prokaryotic cytoskeleton

http://www.ncbi.nlm.nih.gov/pubmed/?term=eukaryotic+cytoskeleton

PMID 26756351

Katherine Ann Hurley, Thiago M A Santos, Gabriella M Nepomuceno, Valerie Huynh, Jared T Shaw, Douglas B Weibel Targeting the bacterial division protein FtsZ. J. Med. Chem.: 2016; PubMed 26756351


Biomed Central

Links

Carnegie stage table

Cell Biology Lecture 1

SMH

testz8600021

Self Reflection

Today in lab I have learnt how to use the Cell Biology wiki page by learning how to make edits and additions to the page. I have also learn shortcut codes to assist me in my future individual assessments and the group assignment. With the ability to make reference through a simple command, it gives me relief that I will not have the problem of the tedious task of finding, copying and pasting references and citings.

Few of the skills I have picked up in this lab class are:

  • Editing my personal Wiki page
    • Creating Headings
      • Making bullet points
  1. Creating hyperlinks to articles and references
  2. Internal links through the use of the header with [[]]
  • The use of the colon, to create hyperlinks to specific pubmed articles through the use of the PMID.

How to make an in-text citation

Bacterial division protein FtsZ.[1]

How to upload an image

Model of hypercoupled cytoskeleton.png]

References

  1. Katherine Ann Hurley, Thiago M A Santos, Gabriella M Nepomuceno, Valerie Huynh, Jared T Shaw, Douglas B Weibel Targeting the bacterial division protein FtsZ. J. Med. Chem.: 2016; PubMed 26756351

Individual Assessments

Lab 1

  • Z8600021 Image Reference, copyright and student template with the image you have uploaded. You do not need to put copyright information on your own page, only in the file summary box. (5/5)


Life and Death Cyanobacterium.png

Figure 1. Life and death of an endosymbiotic cyanobacterium.

Zheng W, Rasmussen U, Zheng S, Bao X, Chen B, Gao Y, et al. (2013) Multiple Modes of Cell Death Discovered in a Prokaryotic (Cyanobacterial) Endosymbiont. PLoS ONE 8(6): e66147. doi:10.1371/journal.pone.0066147[1]

Copyright: © 2013 Zheng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

Lab 2

  • Z8600021 Article does relate to Super-resolution fluorescence microscopy. The summary is far too brief image should be here not at the bottom of your page. (4/5)


Article Summary

This article demonstrates the use of Super-resolution fluorescence microscopy (STORM) to achieve high resolution images Micro-tubule arrays on a Arabidopsis thaliana seedling root. It addresses the current issues with the use of STORM on plant cells due to the high fluorescence background. Even with these issues, the discussions states two methods that are used to help with achieving smaller spatial resolution, which is one of the main goals within this experiment. The two main methods that are discussed are VAE illumination and epi-illumination, both with pros and cons shown in the results.

The conclusion states that the maximum spatial resolution achieved through STORM was up to 20nm. This was achieved with additional techniques, such as enzymatic degradation of the call wall with the combination of freeze-shattering.

Lab 5

CYTOTOXIC LYMPHOCYTES FROM NORMAL DONORS

S Miller Nurse-midwifery in St. Vincent and the Grenadines. J Nurse Midwifery: 1992, 37(1);53-60 PubMed 1538269


Lab 6

  • Z8600021 All information is here, except I asked for the reference to be added here, not referred to from the data sheet. (4/5)


NK Cell and Receptor Antibodies

Mouse anti Human CD16

Mouse derived antibody for humans, specific to the NK Cell CD16. Commercially available as FcRIII as a monoclonal antibody. Group blood protein IgG2a. Application for Immunohistology - Paraffin (1)

References available in the datasheet.

datasheet

Lab 8

  • Z8600021 These are useful peer reviews. Your comments are a little general in some cases and you should be thinking of how each group can use your feedback constructively. (16/20)


Peer review

Group 1 (z3423497)

  • Great introduction, well detailed and gives an insight into the topic.
  • Extensive history but a little too long, key events should be used to condense the timeline. There is also a lack of references for the timeline.
  • Appropriate images are used to further reinforce the information but again there is a lack of references.
  • Additional images for sections such as structure would help greatly in giving readers a visual image of the structures.
  • Information is very clear but there a few spelling errors which can be easily corrected in the final edit.
  • The page does lack a student made image but that can be easily fixed as well.
  • Good use of glossary, is quite extensive.

Overall it is a well written and structured page, additional images and the completion of references will pretty much make the assignment complete.

Group 2 (z3423497)

  • Well written introduction, very easy to understand and is referenced. Only problem is that it is incomplete but that can be easy fixed.
  • Good use of a timeline, not too long and well referenced
  • Each topic has the key information for each heading stated and the corresponding subtopics that cover the structures and functions of the cell.
  • Information flows well and is easy to read as well as understand.
  • There is a lack of images and the addition of images for each of the topics would greatly help in reinforcing the information
  • Some topics can be expanded on such as haemaglobin
  • Few spelling errors and grammatical errors, but this can all be fixed in the final draft.

Overall, it is a very well written page and the information is concise and easy to understand. The addition of few more pictures and the completion of a few paragraphs will make this complete.

Group 3 (z3423497)

  • Great image used for the title banner but lacks the proper referencing and copyright citation.
  • Introduction is detailed but lacks flow. This is present throughout the assignment but can be easily corrected with rearrangement of information.
  • History is a bit short and can be expanded on, there is a lack of referencing.
  • Choice of topics and subtopics is good but some are incomplete, topics such as role in disease and application can be expanded on.
  • More uses of images for the subtopics can help with giving a better understanding of the information and help break down the blocks of text
  • Page lacks a student created image but that can be easily added in, possibly an image could be made for the timeline.

Overall the page has most of the information there, it just needs to be rearranged to create a better flow, additional images would help greatly and references need to be added.

Group 5 (z3423497)

  • Great introduction, well informative and gives a great insight into the topic.
  • Good timeline, well presented and well referenced
  • The overall structure of the topics and the use of images for each subtopic works very well, the addition of the tables also makes it easy to understand the information.
  • Topics are well written and easy to understand, I like how the topics have been split between physiology and pathology.
  • Glossary could be possibly put into a table

Overall it is a very well written page and seems to be almost complete. There use of images to support the information is excellent, there is a great flow in the information and is well referenced. It is evident that the topic has been well researched and that a lot of time has been put in assembling all the information to make it coherent and the assignment coveys it very well, to make it easy to understand.

Group 6 (z3423497)

  • Great introduction, the right amount information to allow the readers have clear idea on what the page is about.
  • Good use of the diagram as well as a youtube video to further help with the understanding of the topic.
  • Headings and subtopics could do with a reorganising, alot of the topics could fall under a greater heading.
  • A lot of information is present but needs to be broken down and simplified for readers to better understand it. Block of texts can be divided into smaller paragraphs.
  • The information could also be rearranged to create a better flow of information down the page so it is easier to understand.
  • Good use of referencing and every thing seems to be well cited.
  • Glossary needs to be completed or removed as well as the final thoughts, don't see the need for a summary on a wiki page.
  • A student image needs to be implemented and could be possibly be done for the comparison of T cells.

Overall the page has all the information, it just needs to be simplified for readers to better understand. This can be achieved by condensing the paragraphs and making it more concise, images can be further added to help show a visualisation of the information, instead of having blocks of texts.

Group 7 (z3423497)

  • Great choice of title image.
  • The addition of a introduction would be great to give an insight into the topic.
  • Nice timeline but it some points could be simplified and the addition of more recent events could further expand on this.
  • Could do with more clearer headings for each topic and could be better structured to make it easier to read and create a better flow of information.
  • The dot points in structure need to be further expanded upon.
  • There are alot of grammatical errors but this can be easily fix,
  • Page lacks a student created image and one could easily be made possibly for the roles of the components, a table of some sort.

Overall the page does seem to be incomplete but is on the right track, it just requires a bit more information for each topic and better organisation of the information it make it more presentable.


References

VAEM-STORM imaging of cortical microtubules in a plant epidermal cell of a whole intact root tip and orientation analysis.[1]
  1. Bin Dong, Xiaochen Yang, Shaobin Zhu, Diane C Bassham, Ning Fang Stochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling Roots. Sci Rep: 2015, 5;15694 PubMed 26503365