Individual Lab Assessments
Lab Assessment 1
Later stage cyanobacterial cell death visualized by the TUNEL assay.
Lab Assessment 2
This Research article explains the achievement of reaching a spatial differentiation which is not limited to light as well as introducing the new and highly technological Super Resolution Florescence Microscopy methods. It addresses the benefits and advances made within the present time and past in regards to optical microscopes leading to the birth of Super resolution fluorescence microscopy techniques. This article focuses mainly on the different branches and types of Super Resolution Microscopy and their different parameters and uses in cell biology observations.
The article addresses the limitations of current optical microscopy, mostly about the diffraction which light undergoes when observing things in high magnification. Super Resolution Florescence Microscopy has managed to overcome these light diffraction barriers providing high resolution imagery as well as being able to observe specimens and structures in a live sample in comparison to other methods.
Lab Assessment 3
Research article breaks down the main components of Elastin describing its characteristics. Observations on the alternating hydrophobic and cross-linking characteristics within Elastin are described. Focuses on explaining in depth the basis of self-organizational ability of elastin-based polypeptides and how the information from them can possibly assist in developing self-assembling biomaterials.
This article focuses on investigating the mechanisms behind the elastic fiber assembly via observing the molecular interactions between 'elastin' and 'microfibrillar' components using solid-phase binding assays. Observations found that the major cross-linking region in elastin is formed by association of domains encoded by exons 10.19 and 25 of tropoelastin.
Lab Assessment 5
Graph representing the phenotypic changes of control B35 cells as well as overexpressed Tm4 (Tropomyosin 4) B35 cells.
Lab Assessment 6
1.Identify an antibody that can been used in your group's extracellular matrix project.
Anti-Elastin Antibody (ab21610)
2. Identify the species deriving the antibody.
3. Identify the working concentration for the antibody.
Application / Dilution
IHC-FoFr 1/50 ~ 1/100
ELISA 1/1000 ~ 1/2000
4. Identify a secondary antibody that could be used with this antibody.
FITC-conjugated secondary antibody
5. Identify a paper that has used this antibody.
Peer Review Assessment
Straight away, the wiki-page is visibly pleasing. The information seems to be organized well under their respective subheadings as well as broken down nicely within their sections to avoid mass clumping of information. It was nice to have an introduction that described simply the function and structure of Proteoglycans, and it was also great to see that you have mentioned which specific topics you will be talking about throughout the page. The history section was used greatly to break down previous, current and future possibilities in research with Proteoglycans as well as a nicely broken down section on structure. I think that the text under the subheading 'structure' can be improved by maybe incorporating some bolding of words (would look simpler and less like a wall of text). Love the table that was used in your project for structure as well as how you broke down the individual components and proceeded to describe them, giving us the reader the most information.
I think maybe you might want to add more information on the synthesis of Proteoglycans, maybe add a video or even a flow diagram, but overall it is well done. I also think that your 'Disease' section is slightly lengthy and too much of a wall of text in comparison to your other sections, the amount of text in this section is overwhelming compared to that in the others (which means you did significant research). Great citation and all your images seem to follow the correct guidelines provided by Mark!
Great job overall! Keep at it!
Having a quick initial look through your project, there seems to be a lot of quality and in-depth information under their respective correct subheadings. The structure section was described in a simple yet very descriptive way allowing me to understand the basic outline of what an Integrin consisted of. There are some parts of this section which is still under construction, nevertheless it is still well written. The function section provides some nice base information with a nice video (there is no sound?) however the video seems rather fast and I had to re-watch it a few times, maybe slowing it down if possible would be great but other than that, the images were great and correctly cited. It was great to see how you talked about Integrin and its interaction with other molecules around it and how it went about in their interactions.
The Disease section is very informative, but it is very hard to read the wall of text. Maybe incorporate images related to the diseases in between the text would be better in breaking up the information, but other than that, you did provide clear concise information on the role of each Integrin in the disease so well done!
In terms of improvement, I think more information on History and Timeline can be provided as well as some information on the synthesis and regulation section of your project. The project is coming together nicely! Well done!
The information in this project seems to be organised well into simple subheadings which allows ease of access for readers to go to their desired section when reading through the project. The introduction has great descriptive information breaking down the structural aspects of Fibronectin as well as lightly touching on the function as well (might be slightly too much for an introduction though). The history section of the project is nicely put, however there seems to be no historical data post 1980s? And also tabulating the data may be aesthetically pleasing.
The structure section is informative and describes the function, form and interaction of all its individual components, this was nicely done, and the break-down of text made it much easier to read and access information. I think this section could be improved by adding a structural image on the side of the text to allow the readers to understand what these components look like or how they interact with one another. This can be said with the assembly section, maybe make use of a flow diagram with your information to show a simplified process of Fibronectin formation.There is abundant information in the Function and Abnormalities section, but as of now it seems like a massive wall of information (good information), which can be addressed by adding in more images in-text or on the side.
Overall, the information is all there on this page! I think it would be nice to add those pictures (you do need a hand-drawn image to meet assessment criteria) to the relevant sections as well as information on the current research to see where we stand on our understanding of Fibronectins. Other than that, great job guys!
Having a quick read through the project, there seems to be an overload of information in all the sections making the wikipage look clustered and hard to access. It would look much more organised if the references at the end of each section were moved to the end of the project rather than being where they are currently. The Introduction section touches on the basics of structure and function, however it would be nice to tell the readers about what your group project will be focusing on throughout the duration of your page.
There seems to be overwhelming information in both the History and Structure sections, which can be fixed by adding subheadings to break down the information further or just to reduce the information in these sections (especially with the structure section which just seems like a wall of text). Summarising the information would be much easier to read. For the functions section, you have broken down Laminins into a whole list of different types and their different function/structures. I suggest making use of a table or even dot points to organise the section better, as well as summarising the information for each Laminin due to the fact that there are so many different types; thus much more information to be added. The ‘Current Research’ section as well as ‘Abnormalities’ can be simplified in the amount of information that is placed within it.
There are some formatting errors that I can see through the page, such as the images not being added correctly (the copyright information should only appear when you click the image for more information, not on the page itself), as well as the reference list being scattered throughout the project (put it at the end of the project. Each different section has a different format of how the text is laid out as well as varying sizes of text, this makes this project seem like it was individuals just placing information on a wiki-page rather than a group assignment. This can be fixed by setting a uniform format that everyone from your group will use throughout the page.Great job on the amount of information that you guys have obtained! Just the formatting and you guys are fine!
The contents menu of the page shows that everything has been categorized and placed under their appropriate sections. There is no introduction as of right now, assuming that the introduction will be added soon, it would be good to address within the introduction the main sections of Collagen Type II that you will be talking about throughout the project giving the reader a basic outline of what is come. The ‘History’ section has some decent information.
The ‘Structure’ section of the project is nicely explained with detailed information regarding its structure and biosynthesis, however I think certain parts, ‘biosynthesis’ can be broken down / summarised to be easily understood by the readers as it is currently a lot of information to take in as well as lacking any in-text citations. Certain subheadings under the Structure section have yet to have information added to them, especially under crucial subheadings such as ‘What cells makes Collagen Type II’ when the project is about Type II Collagen.
Very good job in the ‘Function’ section of the project, however it seems to be lacking in citations and references, unless all the information was obtained from 5 sources of information. The hand-drawn image is nice and simple with the appropriate information blurb under it.The abnormalities section in the project has complex information which might need more organizations and deciphering as to make it easier for the information to be extracted. Possibly make use of dot-points to break up the text as well as images which show the different kinds of abnormalities which arise from Type II collagen diseases.
I think with some formatting, summarizing and more information under certain sections would make the project much more accessible and easy to read / understand. Adding in more images with the appropriate references and citations would also help break-up the text! Great work so far!
Introduction links your project to the ECM which is a great start, great basic information as well as awesome to see that you outline what you will be covering throughout your project! Basic touch on the function as well as interactions with other cells was good to see! The history section is lacking right now but it seems you guys are still working on it, great to see the use of a table in your project.
Current Research section seems to be incomplete? Unless you guys are planning to leave it like that. Might need to work on this part and add some information rather than just placing up PubMed articles. Function section has concise information which is easily understood and accessible due to dot points, there are relevant citations. Seems like lots of research has been done in this section and it is nice to see an addition of a diagram on the side regarding the basic formation of the basement membrane. The Functional layers were informative as well.
In terms of structural components, maybe add a little more information on the structure of the basement membrane if possible before breaking it down into describing the individual components which make it up. The information on each of the individual components is sufficient and not overwhelming, good work on that, however there seems to be some incomplete sections in Structural components which are not complete yet ‘Collagen IV’ and ‘Perlecan’ which will need to be modified. The ‘Function’ section of the project has lots of information but it seems a bit too much like a wall of text? Maybe summarising more of the information, simplifying the data, would make it a lot easier to understand and take in. great In-text citations throughout the project! The Abnormalities are well addressed in the project with their relevant images accompanying the text, the introduction to the Abnormalities was a bonus as well, for describing what kind of abnormalities you will be covering as well the different components of the abnormalities being mentioned.
Overall, great work on your project guys! Just a touchup here and there and you guys are set!
Lab Assessment 12
This research article employed the use of microarray to survey the change in gene expression which occur in the components of cutaneous elastic fibres as a result of intrinsic aging. Real-time polymerase chain reaction and immunostaining was used to determine the age related changes in gene expression (at mRNA and protein levels). The microarray study showed that majority of the elastic fibre network components were unchanged with age
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