User:Z3359076

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Lab Attendance

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Individual Assessments

Lab 1

Single-celled ciliate Tetrahymena thermophila.png [1]

The genome sequence of the single-celled ciliate Tetrahymena thermophila sheds light on early eukaryotic evolution.

Published: August 29, 2006

Copyright: © 2006 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Lab 2

In the research article, "Differentiation of mouse bone marrow derived stem cells toward microglia-like cells" by Arnd Hinze and Alexandra Stolzing, confocal microscopy allowed the researchers to observe the migration of microglia in organotypic brain slices. Confocal images showing either top-down or lateral view of the brain slices were imaged and reconstructed. These images enabled the researchers to study the pattern of cell migration in the brain slices. Confocal microscope scans which were used to determine the depth of cell migration, further improved the precision of the results achieved from this study. [2]

--Mark Hill (talk) 12:22, 11 April 2013 (EST) You have explained how the confocal microscopic analysis contributed to the paper findings and used the Pubmed referencing format correctly. I would suggest in future to add the reference towards the beginning (after research article) and not include the reference or authors in your description.

In the research article[3].....

Lab 3

Laura L Lackner, Jennifer S Horner, Jodi Nunnari Mechanistic analysis of a dynamin effector. Science: 2009, 325(5942);874-7 PubMed 19679814

[4]

This article demonstrates the mechanistic role of DRP-associated proteins using the yeast mitochondrial division as a model. The study explains the specific interaction of protein Mdv1p with GTP-bound Dnm1p and the promotion of Dnm1p assembly onto membranes of mitochondrial, providing clear evidence of the function of Mdv1p in the mitochondrial division mechanism.


Junko Wakabayashi, Zhongyan Zhang, Nobunao Wakabayashi, Yasushi Tamura, Masahiro Fukaya, Thomas W Kensler, Miho Iijima, Hiromi Sesaki The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice. J. Cell Biol.: 2009, 186(6);805-16 PubMed 19752021

[5]

This study demonstrates the physiological function of mitochondrial division in the embryonic and brain development of mice. It also describes the study of the first Dynamin-related protein 1 knock-out mice.


Yusuke Kageyama, Zhongyan Zhang, Ricardo Roda, Masahiro Fukaya, Junko Wakabayashi, Nobunao Wakabayashi, Thomas W Kensler, P Hemachandra Reddy, Miho Iijima, Hiromi Sesaki Mitochondrial division ensures the survival of postmitotic neurons by suppressing oxidative damage. J. Cell Biol.: 2012, 197(4);535-51 PubMed 22564413

[6]

This article provides good findings to prove that mitochondrial division plays a key role in promoting neuronal survival by suppressing oxidative damage in postmitotic neurons. The study of how mitochondrial division affects mammalian cells is one of the latest interests of researchers.


Yusuke Kageyama, Zhongyan Zhang, Hiromi Sesaki Mitochondrial division: molecular machinery and physiological functions. Curr. Opin. Cell Biol.: 2011, 23(4);427-34 PubMed 21565481

[7]

This review article gives an insight to the molecular machinery of mitochondrial division and how mitochondrial division contributes to cellular functions and diseases. It serves as a good starting point for this research project.


JCB 201110034 Fig10.jpg [8]


A model for neurodegeneration caused by mitochondrial division deficiency.

Lab 4

N Cadherin Antibody

Datasheet for N Cadherin Antibody

N Cadherin Antibody is an antibody against all types of cadherin. It is a rabbit polyclonal antibody, costing $299 for 0.2ml. It is applicable in Western Blotting, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. N Cadherin Antibody reacts against a large number of species including amphibians, birds, cows, dogs, fishes, humans, mice, rabbits, rats and Xenopus laevis.

Jason Karamchandani, Megan YiJun Wu, Sunit Das, Hannes Vogel, Paul Muller, Michael Cusimano, Walter Montanera, Kalman Kovacs Highly proliferative sellar chordoma with unusually rapid recurrence. Neuropathology: 2013, 33(4);424-30 PubMed 23082799

[9]

Lab 7

Group 2 Analysis of morphological phenotypes in Tm4 over-expresing B35 neuro-epithelial cells

Group 2 Graph.JPG

1. Do you see any change in phenotypes between group A and group B?

Yes. Majority of the cells in the control group (group B) were either of the broken fan phenotype or the stumped phenotype. In the Tm 4 overexpressed group, majority of the cells were either of the pronged phenotype or the stringed phenotype.


2. If you see a difference, speculate about a potential molecular mechanism that has led to the change.

The overexpression of Tm4 might drive the formation of neutrite and the elongation of cells as the majority of the cells in the Tm4 overexpressed group belonging to either the pronged or stringed phenotype.

Lab 8

Peer Assessments

Group 1:

Overall, the group project page has very good subheadings that would enable one to understand the topic well. However, upon closer look at the content, it seems that the project page is still incomplete especially in the History and Disease section. There also seems to be a lack of references in the content. It’s good that the group balances out the text with pictures. However, the pictures should be formatted properly such that they are in the correct position that they are intended to be on the project. It seems that the pictures are out of place on the page. A short description should also be included with each picture such that it gives the readers a clearer idea of what the picture is trying to show. I like the way the information is presented under the History and Disease section in a table as it makes the content easier to read. The tables are also of a good colour scheme. The information presented was also well reference. I also like that the group has included current and future research sections as it gives a good idea of current issues and interests related to this topic.


Group 2:

The group has broken down the topic into very good sections. Each section is also organised in such a way that it is not overwhelming to read. I also like that the page has a good balance of text and pictures. The pictures that have been included also complemented the content and have clear descriptions that help readers under what the pictures were about. I found the inclusion of the glossary section very helpful as it enables readers to understand the terms that the group had used in their content. However, the introduction, microfilament organisation and animal vs plant cells sections seem to be lacking references. History section also needs to be updated to include more recent events. Overall, a well written and well organised page.


Group 3:

The page is well written and organised under the various subheadings. However, there are some minor grammatical errors though the page and some sections need more references. For the Morphology and Molecular mechanisms section, perhaps the content can be broken down into smaller paragraphs, so it would be easier to read. I like that the group included the Areas of Future Research section, however it seems that the research areas mentioned under the section is more of current rather than future research since the references date from 1998 to 2009? The inclusion of the glossary is also good as it helps readers to understand the terms used in the page better but it needs to be completed.


Group 4:

I like the general structure and flow of the wiki page. There are some minor grammatical errors and formatting can be further improved (using italics for headers can be quite confusing). I also like the use of a general introduction of mitochondria and the use of pictures to summarise. The history section is good in a general overview of cellular biology as it approaches to recent years and narrows on spindle fiber. However, this section is incomplete. The group can perhaps use more subheadings in the structure section to break down that big section into smaller parts in order to help readers better understand what is being discussed. External links should be pushed all to the bottom, under the useful links section as it seems odd to have external links under some sections and have a useful links section as well. The glossary is useful, and can be further expanded to encompass the other complex terms introduced in the page.


Group 5:

This page is very informative and it can be seen that the group members have done extensive research on this topic. However, the introduction section seems too elaborate and too long; this may make readers lose interest in the page. The use of pictures is good but perhaps this group can include more images to balance out the page. There can be more use of subheadings and structuring of paragraphs to make the page more comprehendible. Certain sections such as the Breakdown of the Nuclear Envelop and glossary section appear to be incomplete.


Group 6:

The page is very concise and well-structured. The sections are broken down well so that information under each section is not too overwhelming. However, some pictures used in the page lack descriptions and seem to be out of place. Also, personal notes should be removed from the actual page and perhaps be placed under the discussion page instead. History section is incomplete. Defects from anaphase could be restructured to breakdown the information into subheadings, which will make the information easier to read. Further readings sections should have direct links instead of citations for easy access to the links. The glossary is very detailed and attempts to address more terms than other pages, however this section is incomplete.

References

  1. Richard Robinson Ciliate genome sequence reveals unique features of a model eukaryote. PLoS Biol.: 2006, 4(9);e304 PubMed 20076635
  2. Arnd Hinze, Alexandra Stolzing Differentiation of mouse bone marrow derived stem cells toward microglia-like cells. BMC Cell Biol.: 2011, 12;35 PubMed 21854582
  3. Arnd Hinze, Alexandra Stolzing Differentiation of mouse bone marrow derived stem cells toward microglia-like cells. BMC Cell Biol.: 2011, 12;35 PubMed 21854582
  4. Laura L Lackner, Jennifer S Horner, Jodi Nunnari Mechanistic analysis of a dynamin effector. Science: 2009, 325(5942);874-7 PubMed 19679814
  5. Junko Wakabayashi, Zhongyan Zhang, Nobunao Wakabayashi, Yasushi Tamura, Masahiro Fukaya, Thomas W Kensler, Miho Iijima, Hiromi Sesaki The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice. J. Cell Biol.: 2009, 186(6);805-16 PubMed 19752021
  6. Yusuke Kageyama, Zhongyan Zhang, Ricardo Roda, Masahiro Fukaya, Junko Wakabayashi, Nobunao Wakabayashi, Thomas W Kensler, P Hemachandra Reddy, Miho Iijima, Hiromi Sesaki Mitochondrial division ensures the survival of postmitotic neurons by suppressing oxidative damage. J. Cell Biol.: 2012, 197(4);535-51 PubMed 22564413
  7. Yusuke Kageyama, Zhongyan Zhang, Hiromi Sesaki Mitochondrial division: molecular machinery and physiological functions. Curr. Opin. Cell Biol.: 2011, 23(4);427-34 PubMed 21565481
  8. Yusuke Kageyama, Zhongyan Zhang, Ricardo Roda, Masahiro Fukaya, Junko Wakabayashi, Nobunao Wakabayashi, Thomas W Kensler, P Hemachandra Reddy, Miho Iijima, Hiromi Sesaki Mitochondrial division ensures the survival of postmitotic neurons by suppressing oxidative damage. J. Cell Biol.: 2012, 197(4);535-51 PubMed 22564413
  9. Jason Karamchandani, Megan YiJun Wu, Sunit Das, Hannes Vogel, Paul Muller, Michael Cusimano, Walter Montanera, Kalman Kovacs Highly proliferative sellar chordoma with unusually rapid recurrence. Neuropathology: 2013, 33(4);424-30 PubMed 23082799