User:Z3330795

From CellBiology

Attendance

lab 1--Z3330795 (talk) 15:13, 14 March 2013 (EST)

lab 2--Z3330795 (talk) 15:03, 21 March 2013 (EST)

lab 3--Z3330795 (talk) 15:05, 28 March 2013 (EST)

lab 4--Z3330795 (talk) 15:21, 11 April 2013 (EST)

lab 5--Z3330795 (talk) 15:30, 18 April 2013 (EST)

lab 6--Z3330795 (talk) 15:17, 2 May 2013 (EST)

lab 7--Z3330795 (talk) 16:16, 9 May 2013 (EST)

lab 8--Z3330795 (talk) 16:30, 16 May 2013 (EST)

Lab 1 Assessment

Structure in Field and Cultured Microbialites from the Alkaline Lake Alchichica (Mexico)


Lab 1 Image


Citation: Couradeau E, Benzerara K, Moreira D, Gérard E, Kaźmierczak J, et al. (2011) Prokaryotic and Eukaryotic Community Structure in Field and Cultured Microbialites from the Alkaline Lake Alchichica (Mexico). PLoS ONE 6(12): e28767. doi:10.1371/journal.pone.0028767

Editor: Jack Anthony Gilbert, Argonne National Laboratory, United States of America

Received: September 13, 2011; Accepted: November 14, 2011; Published: December 14, 2011

Copyright: © 2011 Couradeau et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


--Mark Hill (talk) 11:37, 11 April 2013 (EST) See my comments in the image summary box.

Lab 2 Assessment

1. [1]


2. This study by Xiong YS, Yu J, Li C, Zhu L, Wu LJ, Zhong RQ. explored "The Role of Siglec-1 and SR-BI Interaction in the Phagocytosis of Oxidized Low Density Lipoprotein by Macrophages". A confocal microscope was of the highest advantage specifically due to the ability to detect fluorescent stains and give perspective between the protein marked and the rest of the specimen. The advantage of fluorescence in this experiment was being able to attach a flurescent dye to a marker protein Siglec-1 to identify its role and interaction in association with macrophages. Being able to differentiate cells on this level empowers the researcher to observe the relationships between certain cells, without this technology this type of experiment would not be possible.



Citation: Yi-song Xiong,#1 Juan Yu,#2 Chang Li,2 Lin Zhu,3 Li-juan Wu,1,* and Ren-qian Zhong2,*

Editor: Rajesh Mohanraj

Received: October 17, 2012; Accepted February 7, 2013.

Copyright notice: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Go to:


--Mark Hill (talk) 11:42, 11 April 2013 (EST) This paper and your description meets the lab assessment criteria. Though your summary paragraph could have been better formatted as below.

This study[2] used a confocal microscope to specifically detect fluorescent stains and give perspective between the protein marked and the rest of the specimen. The advantage of fluorescence in this experiment was being able to attach a fluorescent dye to a marker protein Siglec-1 to identify its (localisation), role and interaction in association with macrophages. Being able to differentiate cells on this level empowers the researcher to observe the relationships between certain cells, without this technology this type of experiment would not be possible.

Lab 3 Assessment

--Mark Hill (talk) 11:47, 11 April 2013 (EST) You appear to not have yet pasted on discussion page or edited appropriately on the Project page. I have not yet assessed this item, if you wish to improve before the submission deadline.

Mark Hill- please note that we discussed the above issue in class today 11/4/13. I advised you that the below information was on my project page and was mainly under the subheading "metaphase-anaphase transition". You then advised me that this was sufficient for this individual lab assessment. Thank you --Z3330795 (talk) 17:50, 11 April 2013 (EST)

Article 1, Identifies subtle differences between different species mitotic processes Anaphase-promoting complex/cyclosome (APC/C) triggers chromosome isolation along with mitotic exit through selective targeting of cell cycle regulators. Yeast complementation studies composed the nucleus of the investigation however there was no positive result yielded subtlety highlighting servere differences between the 2 different species APC/C’s at review. Further questions have been raised by the negative result whether the parasite used T. brucei even holds MCC-APC/C complex counterpart, this prompted the next part of research comparing the protein characteristics of APC1 fusion proteins at different stages of the cell cycle, the abundant of unchanged proteins indicates that T. brucei has its very own APC/C.[3]

Article 2, Identifies APC role in initiating Anaphase Metaphase-to-anaphase transition is tightly controlled by anaphase-promoting complex (APC) motions. APC promotes the degradation of several proteins that inhibit anaphase, It has been shown that with out destruction of these protein inhibitors the transition is static. [4]

Article 3, Role of regulatory proteins in coordination of Metaphase to anaphase transition The regulatory protein Cdc20 triggers APC/C at the start of the metaphase-anaphase transition. Cdh1 a membrane protein which is able to control the fate of certain cells, triggers anaphase through the G1 stage of the cell cycle. APC/C specifically targets securin(anaphase inhibitor), Early destruction of this inhibitor is coordinated by nuclear transport factors(Nup98 and Rae1), This mechanism is strictly governed to guarantee appropriate timing of degradation. [5]

Article 4, Proves a way to by pass regular cellular requirements by chemical means Kinetochore coordinates chromosome segregation through a binding relationship with microtubules. Sufficient kinetochore-microtubule attachments must be present at the spindle assembly checkpoint however cells can override this regulation through mitotic slippage. Through experimentation It was found when using the anti neoplastic agent nocodazole along with cellular interaction of Cdh1 miotic slippage was achievable. This highlight the critical role of inhibition through destruction in mitotic processes. It must be noted that this experiment could be an insight into a possible future tumour treatment. [6]


Mitotic Slippage - SAC activated

Lab 4 Assessment

My antibody against an adhesion junction protein that is commercially available is Focal Adhesion Associated Protein-Tyrosine Kinase (FAK), This can be purchased from Pierce Antibodies [1] ( a link to the original data sheet page). Applications for use include Immunofluorescence (IF)and Western Blot (WB).Focal Adhesion Associated Protein-Tyrosine Kinase (FAK) is a common Polyclonal(type of antibody), non-receptor protein tyrosine kinase (PTK) (identify the type of adhesion junction). The active site of the protein is centrally located and the carboxyl-terminus pinpoints focal adhesions. Cells are attracted to these sites though the surface integrin receptors. This product has a distinct advantage to its competitors due to a diverse range of species reactivity including Amphibian, Human, Mouse, Rat, Canine(species react in). To my knowledge there was no refferencing available using the above antibody.(reference using antibody).


Lab 5 Assessment

Phenotype A & B Homework data


1) Do you see any change in phenotypes between group A and group B? Group A=Over-expression of Tm4 Group B=Control

Group A, contained many more phenotype D (60)and E(48) in contrast to group B, this group expressed more of phenotype C (77) and D(78)

2) If you see a difference, speculate about a potential molecular mechanism that has lead to the change

The over expression of Tm 4 has structural consequences, this over expression seems to be associated with neurite growth. This is evident between the phenotypes being expressed tending to contain more prominent neurite growth with phenotypes like pronged being hallmarks of such expression.

however the inexperience analysis performed by student may cause less accurate results as the a lot of the structure do look similar for example pronged and stumped.


Lab 6 Assessment

Group 1 -> Regulation of Cell Division

The key points relating to the topic that your group allocated are not clearly described, the introduction should be more simple and a justification of the outline should be provided, ie. why have they chosen those subheadings Content is correctly cited and referenced, however there seems to be a lack of References, perhaps text use could be more efficient leaving out the "waffle" and sticking to shorter sentences.The wiki lacks figures tables and alternative methods of information. There is no evidence of research beyond the formal activities.This group does however relate the topic and content of the Wiki entry to learning aims of cell biology. In Summary the page provides good information however there is no evidence of above and beyond effort, more research needs to be done to establish better understanding for more simplistic explanation/ interpretation.

Group 2 -> Cytokinesis

The key points relating to the topic that your group allocated are clearly described however the information could be broken down into smaller block of text, at first glance looks like essay. The choice of content, headings and sub-headings, diagrams, tables, graphs shows an excellent understanding of the topic at hand, perhaps too much text to describe certain processes. Content is correctly cited and referenced. The wiki has some visual stimulous although they need more of a balance, transforming their obvious extensive knowledge into more bite-size segments, a dramatic increase of non text aids would help. Evidence of significant research is present however different methods of showing this must be applied, any one can summarise information of the internet, please use alternative routes of expressing your knowledge. The group however does relates the topic and content of cell biology extremely well.

Group 3 -> THE GOLGI APPARATUS

This group has the key points relating to the topic clearly described, this group also shows a superior choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area. Content is sufficiently cited and referenced. The wiki element as with most of these projects lack a good balance between text and other means of information. There is significant research relating to basic and applied sciences that goes beyond the formal teaching activities with good use of an external reference heading. Group does relates the topic and content of the Wiki entry to learning aims of cell biology.In summary excellent information however change up your method of interpretation more figures diagrams etc.

Group 4 -> Spindle Apparatus

The key points relating to the topic that your group allocated are clearly & simply described, this already showing a superior layout. The choice of content, headings and sub-headings, diagrams, tables, graphs show a extensive understanding of the topic, an in depth detailed yet simplified for a diverse audience is demonstrated. Content is perfectly cited and referenced.The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations more than other groups however more figures need to be added to trim the text heavy nature of this page. Evidence of significant research relating to basic and applied sciences does goes beyond the formal teachings. Extremely impressive layout and understanding however too text heavy at times.


Group 5 This group has the key points relating to the topic simply described, this group also shows an excellent format of layout and content with an extermely in-depth analysis of their topic. Content is impressively cited and referenced. The common problem is evident again with the wiki element lacking a good balance between text and other means of information. There is an amazing amount of research relating to basic and applied sciences that goes beyond requirements. Group does effectively relates the topic and content of the Wiki entry to learning aims of cell biology. In summary this group is a stand out in terms of depth of information however more emphasis should be on breaking up the large amount of text.


Group 7

The key points are not well identified and there is a lack of consistent structure. The referencing is extensive and reflects significant depth of research. This groups page is extremely text heavy and this aspect of presentation must be addressed. There is obviously alot of research evident but this level of understanding isn't reflected, There must be different vectors to deliver this information. The above and beyond teaching requirements is not evident as this page appears to be quite boring to go through.

Internal Links

Link to first lecture Cell_Biology_Introduction

External Links

favorite personal website [2]


References

  1. Yi-song Xiong, Juan Yu, Chang Li, Lin Zhu, Li-juan Wu, Ren-qian Zhong The role of Siglec-1 and SR-BI interaction in the phagocytosis of oxidized low density lipoprotein by macrophages. PLoS ONE: 2013, 8(3);e58831 PubMed 23520536
  2. Yi-song Xiong, Juan Yu, Chang Li, Lin Zhu, Li-juan Wu, Ren-qian Zhong The role of Siglec-1 and SR-BI interaction in the phagocytosis of oxidized low density lipoprotein by macrophages. PLoS ONE: 2013, 8(3);e58831 PubMed 23520536
  3. Mohamed Bessat, Giselle Knudsen, Alma L Burlingame, Ching C Wang A minimal anaphase promoting complex/cyclosome (APC/C) in Trypanosoma brucei. PLoS ONE: 2013, 8(3);e59258 PubMed 23533609
  4. O Cohen-Fix, D Koshland The metaphase-to-anaphase transition: avoiding a mid-life crisis. Curr. Opin. Cell Biol.: 1997, 9(6);800-6 PubMed 9425344
  5. D J Baker, M M Dawlaty, P Galardy, J M van Deursen Mitotic regulation of the anaphase-promoting complex. Cell. Mol. Life Sci.: 2007, 64(5);589-600 PubMed 17334950
  6. Kazuhiro Toda, Kayoko Naito, Satoru Mase, Masaru Ueno, Masahiro Uritani, Ayumu Yamamoto, Takashi Ushimaru APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage. Cell Div: 2012, 7;4 PubMed 22321970