User:Z3329177

From CellBiology

Welcome to Cell Biology 2017!

Lab 1 Assessment

Lab 2 Assessment

  1. Identify a chemical SDS and the risks and hazards of that chemical in text. Add a link to the original SDS
  2. Select 4 reference papers papers related to your selected group project topic sub-section. Read the research papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page.

Lab 3 Assessment - Endo/Exo worksheet questions.

Lab 4 Assessment

  1. Identify a cytoskeletal antibody.
  2. Identify the species deriving the antibody.
  3. Identify the working concentration for the antibody.
  4. Identify a secondary antibody that could be used with this antibody.
  5. Identify a paper that has used this antibody.

This assessment will be due by the next lab (Lab 5).

Lab 7 Assessment

The following peer assessment exercise should be completed before next lab (Lab 8 - 2 May) as your individual assessment for this week (lab missed due to public holiday).

Your answer should be pasted in 2 places

  1. onto each project discussion page (Note you should add anonymously to the discussion page)
  2. your own individual student page for my assessment.

Each individual will provide a brief assessment of the other groups projects. This should take the form of a brief critical (balanced) assessment identifying both the positive (good) and negative (bad) aspects of the project page as it currently exists online.

You may if you choose, use the final project assessment criteria as a guide. Though you are also welcome to use your own criteria.

Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.

Attandence

Z3329177 (talk) 16:04, 7 March 2017 (AEDT)

Z3329177 (talk) 15:00, 14 March 2017 (AEDT)

Z3329177 (talk) 15:09, 21 March 2017 (AEDT)

Z3329177 (talk) 15:36, 28 March 2017 (AEDT)

Z3329177 (talk) 15:10, 4 April 2017 (AEST)

Z3329177 (talk) 15:20, 11 April 2017 (AEST)

Z3329177 (talk) 15:09, 9 May 2017 (AEST)

Z3329177 (talk) 15:05, 16 May 2017 (AEST)

Z3329177 (talk) 15:09, 23 May 2017 (AEST)

Z3329177 (talk) 15:04, 30 May 2017 (AEST)

Tutorial

Student Image Template

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

Refrencing

https://www.ncbi.nlm.nih.gov/pubmed


Pubmed


Search Plasma Membrane

  • No Link - PMID: 28260162
  • Link - PMID 28260162

Marjut Niinivirta, Gunilla Enblad, Per-Henrik Edqvist, Fredrik Pontén, Anca Dragomir, Gustav J Ullenhag Tumoral cubilin is a predictive marker for treatment of renal cancer patients with sunitinib and sorafenib. J. Cancer Res. Clin. Oncol.: 2017; PubMed 28260162


when you use the reviewed article mention about like as reviewed in, reviewed by ~

I can use the journal which is published 6 months from now.

Individual Assessments

Lab1

Membrane tension negative feedback loop.PNG

Figure title: Membrane tension negative feedback loop


Reference: Alba Diz-Muñoz, Kevin Thurley, Sana Chintamen, Steven J Altschuler, Lani F Wu, Daniel A Fletcher, Orion D Weiner Membrane Tension Acts Through PLD2 and mTORC2 to Limit Actin Network Assembly During Neutrophil Migration. PLoS Biol.: 2016, 14(6);e1002474 PubMed 27280401


Copyright: © 2016 Diz-Muñoz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Lab2

Sigma - Potassium Hydroxide Solution | SDS

Potassium hydroxide solution may be corrosive to metals, harmful if swallowed and cause severe skin burns and serious eye damage or eye irritation.



4 research papers that related to alpha cell.


type 2 diabetes

1. Evolution of Type 2 diabetes, pancreatic alpha cells are exposed to the metabolic stress. Only alpha cell adapt stress and resistance to apoptosis and even increase hormone, while beta cell cannot. This resistance due to the enhanced expression of anti-apoptotic proteins which is Bcl211 and BCl2

Laura Marroqui, Matilde Masini, Beatriz Merino, Fabio A Grieco, Isabelle Millard, Christine Dubois, Ivan Quesada, Piero Marchetti, Miriam Cnop, Decio L Eizirik Pancreatic α Cells are Resistant to Metabolic Stress-induced Apoptosis in Type 2 Diabetes. EBioMedicine: 2015, 2(5);378-85 PubMed 26137583


regulation

2. In mouse pancreatic alpha cells and αTC1.6 cells have histamine H3 receptor. H3R inhibites increasing in intracellular Ca2+ concentration this leads inhibiting secretion from αTC1.6 cells. The research suggest that H3R modulates glucagon secretion from pancreatic alpha cells.

Tadaho Nakamura, Takeo Yoshikawa, Fumito Naganuma, Attayeb Mohsen, Tomomitsu Iida, Yamato Miura, Akira Sugawara, Kazuhiko Yanai Role of histamine H3 receptor in glucagon-secreting αTC1.6 cells. FEBS Open Bio: 2015, 5;36-41 PubMed 25685663


3. HMGN3 is highly expressed in all pancreatic endocrine islet cells, they tested with the Hmgn3-/-mice which have a mild diabetic phenotype, reduced glucagon level in their blood. They examine wether HMGN3 affect glucagon synthesis and secretion in αTC1-9 cells. They found deletion of either HMGN3 or other HMGN variants, no significant affect glucagon gene expression or glucagon secretion. Glucagon secretion does not dependent to HMGN3.

Toshihiro Kurahashi, Takashi Furusawa, Tetsuya Ueda, Michael Bustin The nucleosome binding protein HMGN3 is expressed in pancreatic alpha-cells and affects plasma glucagon levels in mice. J. Cell. Biochem.: 2010, 109(1);49-57 PubMed 19885867


glucagon

4. Glucagon receptor has been identified in most beta cells and subset of α- and δ-cells. So, within the islet, glucagon is likely to function as a paracrine regulator of hormone secretion. Activation of glucagon receptors in β- and δ-cells stimulates insulin and somatostatin secretion through elevation of intracellular cAMP. However the impact of glucagon on alpha cells is difficult to evaluate, test of glucagon receptor knockout mice shows that glucagon is a negative regulator of its own secretion, because they are hyperglucagonemic and have hyperplasia of alpha cells.

Melissa F Brereton, Elisa Vergari, Quan Zhang, Anne Clark Alpha-, Delta- and PP-cells: Are They the Architectural Cornerstones of Islet Structure and Co-ordination? J. Histochem. Cytochem.: 2015, 63(8);575-91 PubMed 26216135


(7/10)

Lab 4

cytoskeleton protein antibody

Smooth Muscle Actin Polyclonal Antibody (PA1-37024)

This antibody recognises rat and human smooth muscle actin, and generated from rabbit.

Suggested secondary antibody. Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Goat anti-rabbit IgG is the suggested secondary antibody and this antibody is conjugated with Alexa Fluor 488.

The working concentration of this antibody is immunohistochemistry (paraffin) (IHC(P))) 1:100

The paper that used Smooth Muscle Actin Polyclonal Antibody is Novel assessment of haemodynamic kinetics with acute exercise in a rat model of pulmonary arterial hypertension. In this research PA1-37024 was used to study effect of the exercise on already-elevated pulmonary arterial perssures and right ventricular wall stress in a rat model of pulmonary arterial hypertension.

(5/5)

Lab 5

Z3329177, Tm4.png

percentage of phenotype comparison of over expressed Tm4 and control B35.

Mark Hill (talk) 20:51, 24 April 2017 (AEST) This lab assessment will be marked by the guest presenter and the mark added here when I have received.

Lab 7

Peer reivew assessment

Group1 delta cell peer review:

The contents are very well organized, the project contains figures and videos, because of that project is easy to understand and straight forward, also the contents are very detailed, especially pathology gives us information about 3 main pathologies. Function part covers very detailed information, this part explains the function and terms to easy to understand.

However, reference 13 is reviewed article, but there is no mention about reviewed article in the paragraph. I might suggest that to easy to read, signaling part could follow after development part, and interaction part could be in the part of the structure with receptor subheading.

So far group 1 put many contents and most references, and when they focus on the editing, the final project will be a very good quality.

Group 2 pancreatic ductal cells peer review:

The project looks very tidy and the order of the sub-heading is nicely organized and It is easy to follow the topic. References used all research article and suitable. Development part contains very much details.

However, more figures or video would help understanding the topic and the image doesn’t have the [Student Image] paragraph, figure number or title might need for easy understanding. The format of the reference needs to change, there is no hyperlink direct to the pubmed. I would suggest that the history part is easy to read when it is in the table. it might help when put more sub-heading of sub-heading.

So far group 2 did well in organizing sub-heading, but title of the figure and right references format is necessary. if put more contents and edit the project, the final work would be better.

Group 3 beta cell peer review:

Project looks very well orgainsed and contains very much details. Introduction is very clear to the topic. reference 1 is reviewed article, but there is mention about reviewed article in the paragraph. History part is well organized and very easy to read and understand. Opening from introduction, history, structure is very easy to read and follow the topic. the figure and the video are heling understanding the topic. it has proper title and references.

However, some figure needs reference and title. Structure part is very detailed, so it might help when it breaks into few sub-headings such as receptor, morphology, location and etc. glossary part may easy to read when It is in the table.

So far group 3 put a lot of work and contents. Little bit of editing would make project look nicer.


Not bad feedback, I guess I was looking for a little more content in your assessment. (6/10)

Group Assessment

Group4

2017 Course Content

Moodle

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2017 Revision

2017 Laboratories: Introduction to Lab | Fixation and Staining |


2017 Projects: Group 1 - Delta | Group 2 - Duct | Group 3 - Beta | Group 4 - Alpha

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G