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From CellBiology

NCAM1

Concentration in normal individuals [1]


CSF: 318.31±14.27 ng/ml Serum: 270.8±8.41 ng/ml


1. (chosen kit) Abcam Australian Destributor: Sapphire Bioscience Pty Ltd Contact info: +61296982022, 1800062088 (free phone) Cost: AUD 417+15 Availability: lead time: 3-4 weeks Sensitivity: ≤10 pg/ml Range: 78 pg/ml – 5000 pg/ml Web Link: http://www.abcam.com/CD56-Human-ELISA-Kit-ab119587.html

Dilution: 1:100 (High target); 1:10 (Medium target); 1:2 (Low target) i.e. concentration range before dilution: 0.16-500 ng/ml

2. Company Name: R&D systems Australian Destributor:Sapphire Bioscience Pty Ltd Contact info: +61296982022, 1800062088 (free phone) Cost: AUD 534.4 +15 Availability: 1-2 weeks Range: 80 pg/ml – 5000 pg/ml

Dilution: CSF: 1:100; Serum: 1:80 i.e. concentration range before dilution: CSF: 8-500 ng/ml; Serum: 6.4-400 ng/ml

3. Company Name: Abnova Australian Destributor:Life Research Contact info: +61 3 9803 0045 Level 1, 5 Lakeside Drive Burwood East VIC 3151 Cost: USD$569 Sensitivity: ≤10 pg/ml Range: 78 pg/ml – 5000 pg/ml Web Link:http://www.abnova.com/de/products/products_detail.asp?Catalog_id=KA2003

Dilution: 1:100 (High target); 1:10 (Medium target); 1:2 (Low target) i.e. concentration range before dilution: 0.16-500 ng/ml

4. Company Name: Adipo Bioscience Australian Destributor:ResolvingIMAGES Contact info: +61 412402229 Cost: USD$395 Range: 46 pg/ml – 3000 pg/ml Web Link:http://www.adipobioscience.com/Product_Systems/NCAM/NCAM.html Note: It is used in CSF or serum free cell culture supernates. No dilution factor can be found.

Clusterin(Apolipoprotein J)

Concentrations in normal individuals

CSF: 5.6±1.1 μg/ml [2] 0.1-1.3 μg/ml [3]

Serum: 55-200 μg/ml [4]

CSF concentration in patients with Alzheimer's disease: 6.5±1.9 μg/ml [5]

1. (chosen kit) BioVendor Australian Destributor:BioCore Pty Limited Contact info: 204 Henderson Road Alexandria Sydney NSW 2015 Australia (61 2) 9565 1281 Email: sales@biocore­.com.au Cost: AUD 890 Availability: 2-3 weeks Sensitivity: 0.5 ng/ml Range: 5-160 ng/ml Web Link:http://www.biovendor.com/product/immunoassays/clusterin-human-elisa Dilution factor: serum:1:3000; CSF: 1:100 i.e. concentration range before dilution: serum: 15-480 μg/ml CSF: 0.5-16 μg/ml


2. Phoenix Pharmaceuticals Australian Destributor:Abacus ALS Australia Contact info: 1(800)222 287 +61 (0)7 33919777 Cost: AUD$581+50 Sensitivity: 1.56 ng/ml Range: 1.56 – 100 ng/ml Web Link:http://www.phoenixpeptide.com/catalog/product_info.php?products_id=10751 No dilution factor can be found.


3. Company Name: USCN Cost: USD$665 Sensitivity: 0.31 ng/ml Range: 0.78-50ng/ml Web Link: http://www.uscnk.com/uscn/ELISA-Kit-for-Human-Clusterin-CLU-945.htm Dilution factor: 500-2000 i.e. concentration range before dilution: 0.39-100 μg/ml Hence, not suitable in measuring serum clusterin levels.

References

  1. Wei Wang, Liang Wang, Jing Luo, Zhiqin Xi, Xuefeng Wang, Guojun Chen, Lan Chu Role of a neural cell adhesion molecule found in cerebrospinal fluid as a potential biomarker for epilepsy. Neurochem. Res.: 2012, 37(4);819-25 PubMed 22219127
  2. A.-M. Lidström, C. Hesse, L. Rosengren, P. Fredman, P. Davidsson, K. Blennow Normal levels of clusterin in cerebrospinal fluid in Alzheimer's disease, and no change after acute ischemic stroke. J. Alzheimers Dis.: 2001, 3(5);435-442 PubMed 12214028
  3. M Polihronis, K Paizis, G Carter, L Sedal, B Murphy Elevation of human cerebrospinal fluid clusterin concentration is associated with acute neuropathology. J. Neurol. Sci.: 1993, 115(2);230-3 PubMed 8387101
  4. Madhav Thambisetty, Yang An, Anna Kinsey, Deepthi Koka, Muzamil Saleem, Andreas Güntert, Michael Kraut, Luigi Ferrucci, Christos Davatzikos, Simon Lovestone, Susan M Resnick Plasma clusterin concentration is associated with longitudinal brain atrophy in mild cognitive impairment. Neuroimage: 2012, 59(1);212-7 PubMed 21824521
  5. A.-M. Lidström, C. Hesse, L. Rosengren, P. Fredman, P. Davidsson, K. Blennow Normal levels of clusterin in cerebrospinal fluid in Alzheimer's disease, and no change after acute ischemic stroke. J. Alzheimers Dis.: 2001, 3(5);435-442 PubMed 12214028