User:Z3324681

From CellBiology

Lab Attendance

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--Z3324681 (talk) 15:20, 6 June 2013 (EST)

Individual Assessments

Lab 1

Bacterial and Eukaryotic Microtubule Structure.png

Structural Model of Bacterial and Eukaryotic Microtubules [1]

Reference: Pilhofer M, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ (2011) Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton. PLoS Biol 9(12): e1001213. doi:10.1371/journal.pbio.1001213

Copyright: © 2011 Pilhofer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

--Mark Hill (talk) 11:06, 11 April 2013 (EST) This image is uploaded correctly with all required associated information, as well as relating to the prokaryotic/eukaryotic topic.

Lab 2

Identify a recent research article (not review) that uses either confocal microscopy or super-resolution microscopy as one of the study's techniques. Explain briefly (1 paragraph) how the microscopy technique specifically contributed to the article's findings.


This study examined the chemotherapeutic drug doxorubicin (DOX) with the novel sixth-generation cationic poly-l-lysine dendrimer (DM) and determined enhanced therapeutic efficacy of DOX when complexed to DM in tumour-bearing mice. Confocal microscopy allowed evaluation of the depth of distribution of both drugs (DOX and DOX-DM) into the Multicellular Tumour Spheroids (MTS)bu the following method. For each image, intensity from background fluorescence was obtained from untreated MTS and subtracted from total intensity. The total fluoresence intensity was then calculated in each region of interest and plotted as a function of the distance from the spheroid rim. [2]


--Mark Hill (talk) 11:20, 11 April 2013 (EST)This paper does employ confocal analysis of tumor growth in vivo. You could have explained why this technique has an advantage/application over other methods. FYI - Live animal fluorescence optical imaging was used to monitor DOX retention in vivo of the fluorescent DOX using the IVIS Lumia II imaging system.

Lab 3

1. Select 4 reference papers related to your selected topic sub-section. Read these papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page. 2. Select an image related to your selected topic sub-section (this can be from one of the 4 above or from elsewhere). The image should be uploaded (with all the required information: description, reference, copyright and student template) and pasted onto the project page sub-section and onto your own personal page.

Lab 4

Identify an antibody against an adhesion junction protein that is commercially available. Add a link to the original data sheet page and identify the type of adhesion junction. Include the following information: type of antibody (polyclonal, monoclonal), species raised in, species reacts against, types of application uses, and if available any reference using that antibody.

Tight Junction Protein 1 Antibody

Original Data Sheer for Tight Junction Protein 1 Antibody

Type of adhesion junction: human zona occludens 1, specifically ZO-1 alpha-minus found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Type of antibody: Polyclonal

Species raised in: Guinea Pig

Species reactivity: Human, Mouse, Rat, and Canine

Types of application uses: Immunohistology, immunofluorescence and Western blotting

References that uses this antibody:

1. Bradley K Wacker, Angela B Freie, Jennifer L Perfater, Jeffrey M Gidday Junctional protein regulation by sphingosine kinase 2 contributes to blood-brain barrier protection in hypoxic preconditioning-induced cerebral ischemic tolerance. J. Cereb. Blood Flow Metab.: 2012, 32(6);1014-23 PubMed 22314269

[3]

2. Andréa M Caricilli, Paty K Picardi, Lélia L de Abreu, Mirian Ueno, Patrícia O Prada, Eduardo R Ropelle, Sandro Massao Hirabara, Ângela Castoldi, Pedro Vieira, Niels O S Camara, Rui Curi, José B Carvalheira, Mário J A Saad Gut microbiota is a key modulator of insulin resistance in TLR 2 knockout mice. PLoS Biol.: 2011, 9(12);e1001212 PubMed 22162948

[4]


Lab 6

Z3324681 Analysis of morphological phenotypes for Lab 6.JPG

Lab 6

References

  1. Martin Pilhofer, Mark S Ladinsky, Alasdair W McDowall, Giulio Petroni, Grant J Jensen Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton. PLoS Biol.: 2011, 9(12);e1001213 PubMed 22162949
  2. Khuloud T Al-Jamal, Wafa' T Al-Jamal, Julie T-W Wang, Noelia Rubio, Joanna Buddle, David Gathercole, Mire Zloh, Kostas Kostarelos Cationic poly-L-lysine dendrimer complexes doxorubicin and delays tumor growth in vitro and in vivo. ACS Nano: 2013, 7(3);1905-17 PubMed 23527750
  3. Bradley K Wacker, Angela B Freie, Jennifer L Perfater, Jeffrey M Gidday Junctional protein regulation by sphingosine kinase 2 contributes to blood-brain barrier protection in hypoxic preconditioning-induced cerebral ischemic tolerance. J. Cereb. Blood Flow Metab.: 2012, 32(6);1014-23 PubMed 22314269
  4. Andréa M Caricilli, Paty K Picardi, Lélia L de Abreu, Mirian Ueno, Patrícia O Prada, Eduardo R Ropelle, Sandro Massao Hirabara, Ângela Castoldi, Pedro Vieira, Niels O S Camara, Rui Curi, José B Carvalheira, Mário J A Saad Gut microbiota is a key modulator of insulin resistance in TLR 2 knockout mice. PLoS Biol.: 2011, 9(12);e1001212 PubMed 22162948


Formatting Experiments

Linking things within the page and renaming said links: Lecture 1 is the same link as Cell_Biology_Introduction

Linking things outside the page and renaming said links: Google it! is the same link as [1]

Pictures: Resizing and repositioning

Red White Blood cells 01.jpg

Pictures: Thumbnail and Renaming

OMGCELLS

Dot points:

  • wow
  • bang
  • pop

Referencing: 1. find the number, remove colon.

PMID 23393914


2. Put pubmed code at front and end.

(pubmed)23393914(/pubmed)


3. Put ref code at front and end

(ref)(pubmed)23393914(/pubmed)(/ref)