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From CellBiology



1.APOE4/Pan-APOE ELISA kit (chosen kit)

- MBL International http://www.mblintl.com/product/7635

Australian distributor: Jomar Bioscience =>AUD$686.00 + $25 delivery + GST


Minimum detection limits:

-APOE: 4ng/ml

-APOE4: 8ng/ml


Dilutions:


-Serum dilution - 1:500 to 1:600

-CSF dilution - 1:50 to 1:100



Normal APOE4/Pan APOE ratio in serum: (obtained from data sheet of ELISA kit)

-APOE4 homozygotic: 1.07 -No APOE4 gene: 0.00 -Heterozygotes: 0.44


Normal levels in CSF:

-APOE: 3-8µg/ml [1], 8.82±3.31mg/L [2]

-APOE4/APOE ratio: 0.6-0.7 (APOE3/APOE4 heterozygotes) [3]




2. APOE3 ELISA kit =>AUD$520-1270 (depending on number of plates) Antigenix America http://www.antigenix.com/Merchant2/datasheet/RHF335CKX1.pdf

Australian distributor: Not available


3. CHGA ELISA kit

-ALPCO Immunoassays http://www.alpco.com/products/Chromogranin_ELISA.aspx

-Australian distributor: Laboratory Diagnostics AUD$1040.00 + AUD$38.00 delivery + GST

-Detection range: 31-830ng/ml

-Normal CSF CHGA: 11.8 +/- 3.0 nmol/ml [4]


  1. C Hesse, H Larsson, P Fredman, L Minthon, N Andreasen, P Davidsson, K Blennow Measurement of apolipoprotein E (apoE) in cerebrospinal fluid. Neurochem. Res.: 2000, 25(4);511-7 PubMed 10823584
  2. K Yamauchi, M Tozuka, T Nakabayashi, M Sugano, H Hidaka, Y Kondo, T Katsuyama Apolipoprotein E in cerebrospinal fluid: relation to phenotype and plasma apolipoprotein E concentrations. Clin. Chem.: 1999, 45(4);497-504 PubMed 10102909
  3. Hiroaki Fukumoto, Martin Ingelsson, Nina Gårevik, Lars Olof Wahlund, Nobuyuki Nukina, Yoshio Yaguchi, Masao Shibata, Bradley T Hyman, G William Rebeck, Michael C Irizarry APOE epsilon 3/ epsilon 4 heterozygotes have an elevated proportion of apolipoprotein E4 in cerebrospinal fluid relative to plasma, independent of Alzheimer's disease diagnosis. Exp. Neurol.: 2003, 183(1);249-53 PubMed 12957508
  4. M Landén, B Grenfeldt, P Davidsson, M Stridsberg, B Regland, C G Gottfries, K Blennow Reduction of chromogranin A and B but not C in the cerebrospinal fluid in subjects with schizophrenia. Eur Neuropsychopharmacol: 1999, 9(4);311-5 PubMed 10422891