User:Z3292208

From CellBiology

Group 5

Adherens Junctions | Discussion

Free to reuse journals: Cell Biology | BMC Cell Biology | Public Library of Science

Lab Attendance

Lab 1 --z3292208 10:37, 10 March 2011 (EST)

Lab 2 --z3292208 09:02, 17 March 2011 (EST)

Lab 3 --z3292208 08:25, 24 March 2011 (EST)

Lab 4 --z3292208 09:27, 31 March 2011 (EST)

Lab 5 --z3292208 08:24, 7 April 2011 (EST)

Lab 6 --z3292208 08:13, 14 April 2011 (EST)

Lab 7 --z3292208 08:48, 21 April 2011 (EST)

No lab 8 - mid semester break.

Lab 9 --z3292208 08:29, 5 May 2011 (EST)

Lab 10 --z3292208 09:36, 12 May 2011 (EST)

Lab 11 --z3292208 08:37, 19 May 2011 (EST)

Lab 12 --z3292208 08:32, 26 May 2011 (EST)

Individual Assessments

Lab 1 Questions

1. What are the key cell biology journals?

  • The Journal of Cell Biology
  • BMC Cell Biology
  • Nature Journals
  • Cell
  • Trends in Cell Biology

2. Which journals allow reuse of their published content?

The Journal of Cell Biology and BMC Cell Biology.

3. Show some formatting.

Bold text.

Italic text.

4. Internal link: First lab

5. External link: The Journal of Cell Biology

Lab 2 Questions

Some links

1. Which chromosomes contribute to the nucleolus?

The tips of five chromosomes contribute to the nucleolus, these are chromosomes 13, 14, 15, 21 and 22. These chromosome sections contain 180 rRNA genes which function to produce ribosomes needed for protein synthesis in the cytoplasm.Nucleolus Pubmed Nucleolus

2. Identify and add a link to your page of a recent cell biology article using confocal microscopy.

Article using confocal microscopy: Shear bond strength, failure modes, and confocal microscopy of bonded amalgam restorations

Lab 3 Questions

1. Find the SDS information for chloroform and identify the hazards associated with this chemical.

Safety data sheet (SDS) for Chloroform

Hazards:

  • Harmful if swallowed.
  • Irritant to the skin.
  • May have a carcinogenic effect.
  • Prolonged exposure by inhalation or swallowing will have serious health effects.

2. You will need to upload an image and add it to your page, with the reference and copyright information with the image.

Mouse zygote.jpg

HGM reveals sub-cellular detail in the living mouse zygote.[1]

Lab 4 Questions

1. Identify a commercial supplier of an antibody that relates to your group project topic.

Atlas Antibodies: Rabbit Anti-Human AJAP1 Polyclonal Antibody, Unconjugated. [2]

2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria?

The gene for Cytochrome C called the CYCS gene is located on chromosome 7. [3]

The Cytochrome C protein is located on the inner membrane of the mitochondria.[4] It is kept within the mitochondria due to its attachment to the membrane by the protein cardiolipin[5] and is only released to the cytosol when apoptosis begins, this release is regulated by Bcl-2 family proteins.


Lab 6 Questions

Phenotype Graph A.PNG


A = Fan

B = Broken fan

C = Stumped

D = Pronged

E = Stringed

F = Pygnotic











1. What are the changes in phenotypes that are observed between group A and group B?

  • Phenotype Fan - Tm4 group reduced by about 11%
  • Phenotype Broken Fan - Tm4 group reduced by about 30%
  • Phenotype Stumped - Tm4 group increased by about 17%
  • Phenotype Pronged - Tm4 group increased by about 26%
  • Phenotype Stringed - Tm4 show only slight increase of about 3%
  • Phenotype pygnotic - Tm4 only show very slight decrease about 1%

Most common phenotype for Tm4 was stumped and pronged and most common phenotype for the control was fan and broken fan.

2. How does Tm4 mediate these changes?

Lab 9 Questions

1. Identify from one of the cell line repositories: a neural cell line and a muscle cell line.

Neural cell line: CRL-2299 from ATCC [6]

Muscle cell line: CRL-1446 from ATCC [7]


2. Identify the species and growth conditions for these cell lines.

Neural cell line: CRL-2299.[8]

Species:

Organism = Mus musculus (mouse).

Morphology = epithelial.

Organ = brain

Strain = BALB/c

Tissue = cerebral cortex

Disease = endothelioma

Cell Type = endothelialpolyoma middle T antigen transformed

Growth conditions:

ATCC complete growth medium = The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature = 37.0°C

Atmosphere = air, 95%; carbon dioxide (CO2), 5%


Muscle cell line: CRL-1446[9]

Species:

Organism = Rattus norvegicus (rat)

Morphology = myoblast

Source = myoblast

Strain = BD1X

Organ = heart

Tissue = myocardium

Growth conditions:

Growth medium = The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Reference List

  1. Tomoko Watanabe, Anisha Thayil, Alexander Jesacher, Kate Grieve, Delphine Debarre, Tony Wilson, Martin Booth, Shankar Srinivas Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopy. BMC Cell Biol.: 2010, 11;38 PubMed 20525231
  2. http://www.biocompare.com/ProductDetails/1233430/Rabbit-Anti-Human-AJAP1-Polyclonal-Antibody-Unconjugated.html?
  3. http://biowww.net/gene/gene-CYCS.html
  4. http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/CellularRespiration.html
  5. http://www.pnas.org/content/99/3/1259.full.pdf
  6. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2299&Template=cellBiology
  7. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-1446&Template=cellBiology
  8. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2299&Template=cellBiology
  9. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-1446&Template=cellBiology


Peer Assessment

Group 1 Synaptic Junctions

  • Introduction is good since you give a good outline about the nervous system and what a synaptic junction is, could say a bit about what the page is going to outline aswell. Big picture like that at the beginning draws the reader in, maybe in the intro write what its actually showing.
  • Good use of pictures throughout the project page to break up the text and keep the reader interested.
  • History is detailed but is there anything occurring from 1981-present?
  • Introduction, history, neurotransmitters headings need referencing!
  • Description of what a synaptic junction is is very clear and easy to understand, especially with the supporting pictures.
  • I dont know what the point of the gallery at the bottom is, those pictures would be more effective put throughout the page.
  • Glossary needs to be added to, every scientific word should be explained here, also should be listed alphabetically.
  • The diseases headings should be bolded more and maybe use some dotpoints in this section to make it easier to read, or put it in a table.
  • Overall, good project headings and pictures.

Group 2 Gap Junctions

  • The introduction gave a good overview of all junctions which gives a good idea of what a junction is before detailing specifically what a gap junction is.
  • Detailed history shows good research.
  • Good array of pictures throughout the whole page, breaks up the information well.
  • Comparison table is very detailed and shows a good amount of research as well as the diseases section which is very interesting especially with the addition of pictures.
  • Glossary needs to be added to.
  • The choice of content, headings and sub-headings, diagrams and tables show a good understanding of the topic area.

Group 3 Tight Junction

  • Very good introduction, giving a good overview of all junctions and then specifically what a tight junction, very informative for the reader. But maybe write a bit more about tight junctions than the other junctions to emphasize what your page will be about.
  • Good history section, is there anything beyond 2005?
  • Maybe in structure could bold some key words like the main proteins occludin and claudin to emphasize this to the reader like you have in molecular components. Maybe make molecular components a subheading under structure as that what your essentially talking about. Use of dot points in this section is very useful.
  • Could split the information in function and "Classification of Epithelia Using Tight Junctions" into dot points to make is easier to read, or even just smaller paragraphs as the big blocks of information is more difficult to take in.
  • Diseases is very well researched and detailed but how are these diseases caused?
  • Glossary needs to be expanded, every scientific term in the page should be explained here.
  • Overall the page is very well researched and presented with a good balance of pictures.

Group 4 Desmosomes

  • Introduction is well set out and informative, good how you start with the different classifications of cell junctions.
  • History section is very well detailed but is there anything from 2004-present?
  • Structure section is very detailed and lots of information, try breaking it up into dot points a bit.
  • Current research could be added to.
  • Glossary needs to be expanded to include all scientific terms in your page arranged alphabetically.
  • External links section to images and videos at the bottom of the page is a good idea but you only have two pictures here which could be integrated into your page. Maybe find a link to a video related to your junction and put it here with a blurb about the link.

Group 6 Neuromuscular Junctions

  • Great hand drawn picture to start with to introduce your topic.
  • Introduction is well outlined and informative.
  • History is well presented and detailed but is there anything from 2004-present?
  • Video is a very good idea to give a visual perspective of how this junction works.
  • Overall very informative mechanism of action.
  • Table of components needs some references.
  • Not sure how benefits of light and electron microscopy and differences between scanning and transmission EM have to do with your junction but the explanation of those images of NMJ is very good and shows what it really looks like.
  • Lots of effort and detail has been put into the muscle components and fibres but yet again I feel it strays a bit from the main focus of your page which should be the junction.
  • Diseases table is great, pictures are interesting.
  • For your external links maybe write a sentence next to each one saying what it is.
  • For some of your pictures you have uploaded don't forget to put in the link to the website where you got it from. Also your picture for Botulism doesnt actually say the copyright permission.
  • Hand drawn diagrams are awesome, great effort!
  • Overall a great project, lots of research and effort to make a very informative, well structured and well outlined page.