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From CellBiology

Lab Attendance

--Elizabeth Blanchard 10:42, 10 March 2011 (EST)

--Elizabeth Blanchard 10:53, 24 March 2011 (EST)

--Elizabeth Blanchard 10:32, 31 March 2011 (EST)

--Elizabeth Blanchard 09:35, 7 April 2011 (EST)

--Elizabeth Blanchard 10:32, 14 April 2011 (EST)

--Elizabeth Blanchard 09:12, 21 April 2011 (EST)

--Elizabeth Blanchard 09:35, 5 May 2011 (EST)

--Elizabeth Blanchard 09:35, 12 May 2011 (EST)

--Elizabeth Blanchard 10:12, 19 May 2011 (EST)

--Elizabeth Blanchard 09:24, 2 June 2011 (EST)

Individual Assessments

Lab 1

1. What are the key cell biology journals?

The Journal of Cell Biology

BMC Cell Biology

Cell

Journal of Cell Science

Molecular Biology of the Cell

2. Which journals allow reuse of their published content?

The Journal of Cell Biology

BMC Cell Biology

Public Library of Science

Lab 2

1. Which chromosomes contribute to the nucleolus?

The nucleolus contains repeats of ribosomal RNA genes on chromosomes 13, 14, 15, 21 and 22.

2. Identify and add a link to your page of a recent cell biology article using confocal microscopy.

Biotherapeutic target or sink: analysis of the macrophage mannose receptor tissue distribution in murine models of lysosomal storage diseases.



Lab 3

1. Find the SDS information for chloroform and identify the hazards associated with this chemical.

Chloroform is harmful when inhaled, and in high concentrations may even be fatal. It is a chlorinated hydrocarbon, which toxic when swallowed, and with repeated exposure may cause dermatitis and reproductive harm. May also be a potential human carcinogen with repeated exposure.

Chloroform Safety Data Sheet

Natural cycle and OC cycle follicles.jpg

Rebecca L Birtch, Angela R Baerwald, Olufemi A Olatunbosun, Roger A Pierson Ultrasound image attributes of human ovarian dominant follicles during natural and oral contraceptive cycles. Reprod. Biol. Endocrinol.: 2005, 3(1);12 PMID:15829004 | PMC1087505 | Reprod Biol Endocrinol.


© 2005 Birtch et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Lab 4

1. Identify a commercial supplier of an antibody that relates to your group project topic.

Millipore is a commercial supplier of the antibody 'Anti-Connexin 43, C-terminus' related to gap junctions. This specific connexin is able to recognize proteins who have a molecular weight between 43-47kD from Western blots of mouse fibroblasts as well as Cx43 in a variety of organisms.

Millipore: Anti-Connexin 43, C-terminus

2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria?

The CYCS gene encodes for Chromosome C. This gene is located from base pair 25,158,269 - 25,164,979 on chromosome 7. It is associated with the initiation of apoptosis as well as being part of the electron transport chain in the mitochondria. The mitochondrial membrane (in particular- the outer membrane) is reponsible for keeping this protein trapped within the mitochondria to function effectively.


Lab 6

Group 2 analysis graph.JPG

A) What are the changes in phenotypes that you observe between group A and group B?

- In Genotype A, alot more branching and processes are seen. More branching therefore leads to more interaction between the cells. The processes also fluoresce more brightly as compared to Genotype B which are less brightly stained. Genotype B presents much less branching and processes and therefore have less interaction between each other.

- Phenotypes D and E (pronged and stringed respectively) were by far the most dominant phenotypes in both group A and B. Fan, broken fan and pygnotic followed similar trends in both groups A and B, but group A seemed to have the most in those categories as a percentage overall. Group B proved to have over double the percentage of Stumped phenotype group when compared to group A.

B) How does Tm4 mediate these changes?

Tropomysin (including Tm4) regulates the structural properties of the actin cytoskeltons in neurons. Over expression of the Tm4 cell leads to more branching as compared to the control cells, B35. The over expression of the protein is also able to induce elongation and increase neurite length. By Tm4 mediating these changes, physical changes in the appearance of the neurones can be observed. These can be clearly visible when comparing to the control cells as more branching and longer processes are seen.

Lab 9

1. Identify from one of the cell line repositories: a neural cell line and a muscle cell line.

Muscle Cell Line: Myoblast Myoblast

Organism: Rattus norvegicus (rat) Tissue: skeletal muscle Cell Type: myoblast


Neural Cell Line: Neuroblastoma Neuroblastoma

Organism: Homo sapiens (human) Organ: brain Derived from metastatic site: abdominal mass

2. Identify the species and growth conditions for these cell lines Muscle Cell Line: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: The myoblastic component of this line will be depleted rapidly if the cells are allowed to become confluent.

Neural Cell Line ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C

Lab 10

Peer Review of Group Assessments

Group 1

Good choice for initial picture. Introduction could include a little bit more about what you will talk about.

History is too short and everything should be referenced. More research should be done to find out more recent findings.

Hand drawn pictures are lovely and complement the project very nicely. Obviously a lot of time was put into these, nice work!

Types of synaptic junctions is very concise.

Good balance between text and pictures in synaptic integration and modulation.

The use of tables allows for clear understanding.

Diseases are well researched, however they are very detailed and seem to be form a pathological perspective. Summarizing these and just putting the main relevant points


Group 3

Good concise introduction.

History well balanced with the addition of pictures.

Molecular components is detailed, but readable.

Spelling error in one of your subheadings in function.

Content in classification of epithelia is good, but it gets very tiring reading this so try breaking this up.

Very extensive table of diseases but how do tight junctions actually cause these??

Might also be worthwhile adding to your glossary, but overall good project.


Group 4

Overall, maybe add a few more pictures throughout to break up the text.

Intro: Introduction is a little short- try adding some information about what you will be talking about in more detail. A picture would also compliment this nicely.

History: Obviously well researched and referenced.

A few details in the structure need to be simplified.

Regulation is a good topic choice and nicely set out.

Current research could be extended. But good work overall!


Group 5

Introduction and History are both too short and an initial picture of adherens junction would be nice.

Structure and function are both thorough- although adding a picture to function would make it a little more interesting and would break up the text.

Importance and Regulation as well as Diseases are very well balanced with pictures and text and are well written.

Current research and the table are both very extensive. Nice project overall.


Group 6

There a a few spelling mistakes in your page, maybe just do a quick spell check

Need to complete history section, maybe up until 2005

Some pictures arent referenced eg embryonic development

Loved the videos

Good referencing

Good tables to break up text

The overall page is awesome, obviously a lot of hard work and research has gone in to it. Well done guys

Work Area

Lab 1

Bold Text.

Italic Text.

First Lab

Journal of Cell Biology Homepage

Lab 2

http://www.ncbi.nlm.nih.gov/pubmed/

Pubmed


Lab 3

This is about frog nucleoli shape changes.[1]

Peer Review

Group 6:

There a a few spelling mistakes in your page, maybe just do a quick spell check

Need to complete history section, maybe up until 2005

Some pictures arent referenced eg embryonic development

Loved the videos

Good referencing

Good tables to break up text

The overall page is awesome, obviously a lot of hard work and research has gone in to it. Well done guys


Reference list

  1. Clifford P Brangwynne, Timothy J Mitchison, Anthony A Hyman Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes. Proc. Natl. Acad. Sci. U.S.A.: 2011, 108(11);4334-9 PubMed 21368180