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From CellBiology

OHS

OHS is extremely important in lab work. The potential exposure to chemical, radioactive, inflammable and other dangerous substances means that proper understanding of the dangers and ways in which it can be prevented is extremely vital for OHS.

The lab is comprised of 2 rooms, G20 and G22. G22 door is always locked, and access must be through G20. Before entering, check if the red light is flashing, which indicates a high level of CO2 inside the room. Upon entering, first thing to do is wash hands and sign in via lab diary. Beside the lab diary, there is a first aid kit and chemical safety data sheet. Protective clothing (gowns) and goggles are worn to protect from splashing of chemicals. However, if it does occur, there is a shower and tap near the door to wash off chemicals from eye or clothes.

Tissue Culturing

The biggest problem facing tissue culturing is contamination, which greatly influences results. In order to reduce this, the biohood is equipped with UV light, which can sterilise the work area. Ethanol (70%) is used to clean the workplace, and the air used goes through a filter that prevents anything over 0.2 microns from passing through, and the air is also filtered before it leaves.

In terms of working in the labs, the rooms are to be booked online prior to use. Before commencing work, it is recommended all the equipments are prepared, preheated, and set up. External surfaces can be cleaned by 70% ethanol. Gloves are used to prevent contamination or exposure to chemical and biological wastes. Equipment is generally placed in a way that frees up work space in the middle of the biohood, with equipment used on the sides of the work space.


PMID 20043966