User:Z3284634

From CellBiology

Lab Attendance

--Z3284634 (1) 15:13, 8 March 2012 (EST)

--Z3284634 (2) 14:09, 15 March 2012 (EST)

--Z3284634 (3) 14:07, 22 March 2012 (EST)

--Z3284634 (4) 14:07, 29 March 2012 (EST)

--Z3284634 (5) 14:18, 5 April 2012 (EST)

--Z3284634 (6) 14:06, 19 April 2012 (EST)

--Z3284634 (7) 14:01, 26 April 2012 (EST)

--Z3284634 (8) 14:37, 3 May 2012 (EST)

--Z3284634 14:15, 17 May 2012 (EST)

--Z3284634 14:37, 24 May 2012 (EST)

--Z3284634 15:19, 31 May 2012 (EST)

Lab 1

[1]

Cell Biology Introduction

Lab 2

Ribosomes, or particles of Palade, in rat pancreas.jpg


Superresolution Microscopy

Helge Ewers Septin pairs, a complex choreography. J. Cell Biol.: 2011, 193(6);959-61 PubMed 21670210


Septin complex.gif

In this article, super-resolution microscopy was used to localize and focus (right red points) on more minute details. Whereas, normal microscopy is only limited to a few hundred nms magnification (left red point). Super-resolution microscopy can focus to the point of or less than 100nm, which is useful when researching into the structures of a cell.


Lab 3

Formaldehyde (Formalin)

Dangerous goods class: 8

Hazard Category: Harmful, Corrosive

Risk

Toxication by inhalation

Causes burns

Possible risk of irreversible effects

Risk of serious damage to eyes

May cause sensitisation by skin contact


Insulin Signalling Pathway

1). Frédéric Tremblay, AnneMarie Gagnon, Alain Veilleux, Alexander Sorisky, André Marette Activation of the mammalian target of rapamycin pathway acutely inhibits insulin signaling to Akt and glucose transport in 3T3-L1 and human adipocytes. Endocrinology: 2005, 146(3);1328-37 PubMed 15576463


This article discusses the inhibition and activation of insulin signalling through activating / inhibiting rapamycin pathway and in turn, the activated, inhibited receptors.


2). Malcolm A Leissring, Enrico Malito, Sabrine Hedouin, Lael Reinstatler, Tomoko Sahara, Samer O Abdul-Hay, Shakeel Choudhry, Ghulam M Maharvi, Abdul H Fauq, Malwina Huzarska, Philip S May, Sungwoon Choi, Todd P Logan, Benjamin E Turk, Lewis C Cantley, Marika Manolopoulou, Wei-Jen Tang, Ross L Stein, Gregory D Cuny, Dennis J Selkoe Designed inhibitors of insulin-degrading enzyme regulate the catabolism and activity of insulin. PLoS ONE: 2010, 5(5);e10504 PubMed 20498699


The possibility of enhancing the activity of insulin through insulin degrading enzyme is discussed in the review article. This is to research further into the different pathway insulin signalling affects and how it is carried through.


3). Jiaqi Huang, Chris Morehouse, Katie Streicher, Brandon W Higgs, Jin Gao, Meggan Czapiga, Anmarie Boutrin, Wei Zhu, Philip Brohawn, Yong Chang, Jaye Viner, Theresa LaVallee, Laura Richman, Bahija Jallal, Yihong Yao Altered expression of insulin receptor isoforms in breast cancer. PLoS ONE: 2011, 6(10);e26177 PubMed 22046260


Insulin Growth factor signalling through insulin isoforms that contribute to breast tumors/cancers - allows a deeper understanding into the results of altered insulin receptor expression.


4). Kimberly X Mulligan, R Tyler Morris, Yolanda F Otero, David H Wasserman, Owen P McGuinness Disassociation of muscle insulin signaling and insulin-stimulated glucose uptake during endotoxemia. PLoS ONE: 2012, 7(1);e30160 PubMed 22276152


Muscle reactions to insulin receptor signaling and the uptake of glucose during endotoxemia, stimulated by insulin.


Lab 4

Jun Muto, Takao Imai, Daisuke Ogawa, Yoshinori Nishimoto, Yohei Okada, Yo Mabuchi, Takeshi Kawase, Akio Iwanami, Paul S Mischel, Hideyuki Saya, Kazunari Yoshida, Yumi Matsuzaki, Hideyuki Okano RNA-binding protein Musashi1 modulates glioma cell growth through the post-transcriptional regulation of Notch and PI3 kinase/Akt signaling pathways. PLoS ONE: 2012, 7(3);e33431 PubMed 22428049

[1]

The Mushashi family of RNA-binding protein consists of MSI1 [RNA-binding Protein critical in nervous system development and stem cell self-renewal], MSI2.


Musashi1 Antibody (ab21628)

Rabbit polyclonal to Musashi1

Reacts with Mouse and humans

Applications: ICC/IF concentration of 1microgram/mL

WB concentration of 1microgram/mL

[2]


Secondary Antibody

[3]

Alexa Fluor 488 - Goat(host) anti Rabbit (reactivity)

Targets IgG isotope


Lab 6

Table 1

Cell counts.JPG

Questions

1. Do you see a difference in phenotype between Tm4 over-expression and control cells?

Yes

Tm4 consists a majority of stumped and pronged phenotypes, whereas, broken fans and fans are more common in the control samples.


2. If so, how could Tm4 over-expression lead to this difference?

The increased number of stumped and pronged phenotypes present in the Tm4 sample suggests that Tropomyosin 4 over-expresses in phenotypes that has more branching processes, thus more interaction between phenotypes.

Part 2 - cAMP

Genotype A

- More processes and branching [more interaction]

- Lighter periphery staining

- the major phenotypes are stumped and pronged


Genotype B

- Less Branching [less interaction]

- Lighter periphery staining

- consists of more broken fan phenotypes


Lab 7

Contributions to date:


Normal Function (partial)

Abnormal Function (partial) "defects in myocardial signalling"

History (discussion) - havent yet transferred to page


Lab 8

1. Identify a mammalian cell line in the ATCC catalogue (and add a link)

ATCC No. -- CCL. 7.2

Designations: NCTC clone 3526 [4]

2. Identify the original tissue of origin of that cell line.

Epithelial cells from the kidney of Macaca mulatta (Rhesus Monkey)

3. Identify the original paper that characterised the properties of that cell line.

V J EVANS, J C BRYANT, H A KERR, E L SCHILLING CHEMICALLY DEFINED MEDIA FOR CULTIVATION OF LONG-TERM CELL STRAINS FROM FOUR MAMMALIAN SPECIES. Exp. Cell Res.: 1964, 36;439-74 PubMed 14242231


Lab 9

1

Introduction

  • Brief, but detailed insight into Testosterone Signalling

History

  • The table layout is great and well referenced, however, the colour might not be too appropriate

Biosynthesis & Regulation

  • I thought these two parts were easy to follow, although biosynthesis was a bit hard to understand. Probably could have included a bit about the Bolded words.

Signalling Pathway

  • I thought this section was nicely structured. It flows, with relevant images and introduces the classical and non classical pathways which ties nicely to the next section.

Normal & Abnormal Function

  • The section for Normal function could have added some sub titles so the readers won't feel too overwhelmed with the extensive texts, but it was informative. The abnormal function provided interesting introduction into the different outcomes.

Clinical uses & Current Research

  • Provides an informative and insightful understanding as to how this relates to the bigger picture. I thought it was a good conclusion to the project.


2

Introduction

  • Maybe a bit more info would be good, slightly brief

History

  • Good use of table, well referenced and the colour choice was good, not too bright.

Normal & Abnormal Function

  • I thought normal function should come just before abnormal function so readers could have a better comparison between the two, and the signalling pathway could be better placed just after the introduction. The table in abnormal function was great, with the relevant images placed, was really helpful.

Signalling pathway

  • I thought that maybe the information in this section was a brief, could use a bit more information. Also, it would be nice if there were some info under VEGF, so it won't seem like theres nothing done on it.

Research

  • The information was well researched, but the layout wasn't as clear. Maybe the pictures should all go on one side of the page, to present a clearer page.

Glossary

  • Better placed before the Reference, as readers might miss it.

--Mark Hill 13:34, 17 May 2012 (EST) You have not completed the peer assessment process yet. If you have made comments on each project page they need also to be pasted here today for me to include in your individual assessment.

3

Introduction

  • could be a bit more brief, just list the components that will be discussed.

History

  • good information however, reference needed

Signalling pathway

  • could do with a bit more info and pictures would help with explaining how it works

Function

  • The information flows but not much structure. Sub headings would help give a clearer understanding. Abnormal function?

Current Research

  • Again, headings would give readers a better understanding of what they are reading, but good articles and examples

Glossary and References could do with some work

4

Introduction

  • Clear cut and straight to the point

History

  • a bit too brief

Pathway, Proteins and Receptors

  • I thought the pathway was well explained, but more detailed description would be better. Also, proteins and receptors might be better as a subheading under pathways? The image is good, but maybe should include a brief description

Normal Function

  • This section is well researched, clear layout and the flow chart helped in explaining about the role of Notch in CNS. Each sub section has a nice intro and, brief detailing and closing, overall i thought this section was good.

Abnormal Function?

Further Research

  • Lack of description on each article, even a brief intro on what they are researching on would be good


5

Introduction

  • A great introduction to the topic, it incorporates the information that would be discussed

History

  • I thought it was really good as it is well referenced with links to abbreviations

Mechanisms of action

  • Even though it was in point form, it was clear in explaining what it does and how. I thought it was refreshing as it is a bit different from others, and doesn't seem like information overload. Relevant image with description, easy to understand.

Diseases

  • Good use of table and images to present the different type of disease. Good amount of information on each disease, brief but informative

Key players

  • again, table gives a nice layout, and information given shows extensive research done

Overall, i quite like the layout and the amount of effort put into this page, as it shows enough information but not too much, and glossary links and the list of terminology provide an easy read. Well referenced.

7

Introduction

  • Intro should be brief and the pathway discussed should have a separate heading. I liked the drawing, its easy to process and follow, although the references were a bit confusing, would be better if it was in text citation.

History

  • Well written and referenced

Gene Description & Receptor Agonists

  • Lacking a bit of reference, Betas 1,2& 3 could use a bit more info. The table for Receptor Agonists is too bright but table layout is good.

Structure

  • Very detailed section, with relevant images,

Pathway & Normal function

  • good image albeit a bit confusing with lots of arrows but i understand its the pathway thats hard to grasp and not the picture Great job. This section is well written and well explained.

Abnormal function

  • great use of table - information easy to absorb. Very well researched and interesting topics on SCD and DCM

Glossary

  • Sufficient info on each term to provide a good understanding to the overall topic


8

Introduction

  • Brief but introduces the topics that will be discussed nicely

Pathway

  • The process runs nicely with the image, however, more information would show a bit more depth in the understanding of the topic

History?

Normal Function & Abnormal Function

  • Block of information, probably could use lists in some cases, (new line for 1, 2, 3, as it gives a bit more structure and won't seem to boring) Needs referencing, and some images would also help.
  • Maybe a table would help?

Proteins

  • Again, lots of good information, but its all in a block. Readers might get bored with tons of info, so structuring it out a bit would help, also adding images could lighten up the page

Need some info on current research, but overall theres a lot of info, just need to do some work on structuring

9

Introduction

  • Good intro, could end with what you'll be discussing throughout the page

Pathway

  • sub headings for the different pathways and then more information on each. More referencing needed

History

  • well researched and referenced, although i think History should come just after introduction, so its like a background to what is being researched.

Current research

  • needs more work, and best placed at the end, just before glossary, a good wrap up

Normal Function

  • Well researched, but a bit more info under each heading to show more knowledge on the topic

Abnormal Function

  • lack of information, and should talk more about the progression to carcinoma

Glossary

  • well done

Lab 12

  1. Identify a current technique used in gene sequencing.

Next Generation sequencing

  2. Identify a recent cell biology research paper that has used microarray technology.

Zahia Hamidouche, Olivia Fromigué, Jochen Ringe, Thomas Häupl, Pierre J Marie Crosstalks between integrin alpha 5 and IGF2/IGFBP2 signalling trigger human bone marrow-derived mesenchymal stromal osteogenic differentiation. BMC Cell Biol.: 2010, 11;44 PubMed 20573191


  3. What aspect of the research findings were contributed by the microarray technique.
  • Mesenchymal Stromal Cells were incubated and total RNA was isolated
  • Gene expression profiling was performed in different studies using human Mesenchymal stromal Cells
  • cRNA was synthesized from total RNA and transcribed
  • Fragmented cRNA was then hybridized to GeneChips
  • GeneChips were then washed, stained and scanned with GeneArray scanner
  • Raw Gene data were then processed for signal calculation
  • Jun Muto, Takao Imai, Daisuke Ogawa, Yoshinori Nishimoto, Yohei Okada, Yo Mabuchi, Takeshi Kawase, Akio Iwanami, Paul S Mischel, Hideyuki Saya, Kazunari Yoshida, Yumi Matsuzaki, Hideyuki Okano RNA-binding protein Musashi1 modulates glioma cell growth through the post-transcriptional regulation of Notch and PI3 kinase/Akt signaling pathways. PLoS ONE: 2012, 7(3);e33431 PubMed 22428049