User:Z3269335

From CellBiology

Hi. I am Rosita, currently doing Medical Science in UNSW. I am interested in doing Medical Research and I am currently seeking Honours Projects in Cell Biology.

--Rosita Pang 05:35, 11 March 2010 (UTC)

Lab 1 - What Cell Biology Journals allow reuse of acknowledged and correctly cited content?

The Journal of Cell Biology, BMC Cell Biology and Public Library of Science allow reuse of content provided the article is properly cited.

ANAT3231_References

--z3269335 06:47, 10 March 2010 (UTC)

Lab 2 - What are the "light" regions of DNA within the nucleus called?

Euchromatin

http://www.cytochemistry.net/Cell-biology/Nucleus.htm

--Rosita Pang 06:47, 17 March 2010 (UTC)

Lab 3 - How do we know the hazards associated with research chemicals?

We can find out the hazards associated with research chemicals by looking it up from the Material Safety Data Sheets (MSDS). We can also go to the website: UNSW Chem Alert and type in the research chemicals' names, then a detailed list of hazards associated with the chemicals would be found.

--Rosita Pang 06:39, 24 March 2010 (UTC)

Lab 4 - Identify a commercially available antibody to an adhesion junction protein, and also add a link to the antibody page on your own student page.

Desmoplakin I+II antibody [2Q400]

  • Mouse monoclonal [2Q400] to Desmoplakin I+II
  • Reacts with Human, Mouse, Rat, Chicken, Cow
  • Product type: Primary antibodies
  • Immunogen: Full length protein (Cow).
  • Tested applications: ICC/IF, IHC-Fr, WB
  • Use: Suitable for use in the detection of primary and metastatic carcinomas.

http://www.abcam.com/Desmoplakin-I-II-antibody-2Q400-ab16434.html

--Rosita Pang 06:59, 31 March 2010 (UTC)

Lab 6 - Analysis of morphological phenotypes in Tropomyosin 4 over-expressing B35 neuro-epithelial cells

Z3269335 Lab6.JPG

--Rosita Pang 07:24, 21 April 2010 (UTC)


Lab 7 - Confocal Microscopy

From today's confocal tutorial, identify an advantage to using each of the following objectives: Air, Water or Oil.

Although air allows the lowest magnification (20X, 40X), with working distance around 5mm, the lens would not be contaminated and haziness is reduced.

Water allows a greater magnification (60X) than air while keeping the cell alive under a moist environment. Its working distance is shorten, around 0.28mm. Also, it reduces spherical aberration.

While, the use of oil allows the highest magnification (60X, 100X), with working distance at around 0.20mm only. Compared with air and water, oil immersion lenses allow a better optical performance with a higher resolution.

--Rosita Pang 07:53, 28 April 2010 (UTC)

Lab 8 - Tissue Culture/ Peer reviewed of others' projects

Group 1 Project - Fluorescent-PCR

--z3269335 07:40, 5 May 2010 (UTC)

Good points:

  • It is nice to see that the group has included sufficient diagrams and some self-drawn diagrams, which help explaining a complex idea.
  • Most diagrams include appropriate references.

Things to improve:

  • Missing reference one of the picture: PCR.png.
  • A glossary could be included.


Group 2 Project - RNA Interference

--z3269335 09:49, 11 May 2010 (UTC)

Good points:

  • The project explain RNA interference technique precisely and clearly.

Things to improve:

  • Correct citations and short descriptions for the pictures Z3216273-Petunia-RNAi.png and Z3216273-authors.jpg should be included


Group 3 Project- Immunohistochemistry

--z3269335 10:45, 11 May 2010 (UTC)

Good points:

  • An organized layout with some tables and diagrams helping in explaining the complex mechanism of immunohistochemistry.

Things to improve:

  • Lack of correct citations for some of the content and images.


Group 4 Project - Cell Culture

--Rosita Pang 11:06, 11 May 2010 (UTC)

Good points:

  • The language used in your project could be easily understood.
  • It is nice to see all the colourful pictures.
  • It is a good idea to include helpful links in the project.

Things to improve:

  • Instead of drawing the "self=drawn" diagram with hands, it maybe more professional to draw it using the computer.


Group 5 Project - Electron Microsopy

--z3269335 11:40, 11 May 2010 (UTC)

Good points:

  • Lots of colourful, properly cited diagrams and pictures.
  • A table is used to compare and contrast Transmission Electron Microscope and Scanning Microscope.

Things to improve:

  • The pictures TEM Diagram.jpg and SEM Diagram.jpg should clearly indicate that they are "self-drawn diagrams" under the comment of the image if they are drawn by the students. If not, a proper reference should be included.
  • A glossary could be included at the end of the project too.


Group 6 Project - Confocal Microscopy

--z3269335 14:03, 11 May 2010 (UTC)

Good points:

  • It is clear and consize with properly cited diagrams.
  • Professional computer drawn diagram that explained the machanism involved in Confocal Microscopy.
  • It is also a good idea to add the hyperlinks to the glossary.

Things to improve:

  • For the timeline and development, instead of presenting it in paragraphs, making it in dot-points form/ a table form may make it clearer.


Group 7 Project - Monoclonal Antibodies

--z3269335 14:24, 11 May 2010 (UTC)

Good points:

  • The group seems to have done a detailed research on monoclonal antibodies.

Things to improve:

  • The diagram Monoclonal antibody development.jpg has not been correctly referenced. If it is a self- drawn diagram, it would be nice if it is clearly stated in the comment.
  • It may be a good idea to unify the way of referencing, so that the project would look more professional.


Group 9 Project - Fluorescent Proteins

--z3269335 14:42, 11 May 2010 (UTC)

Good points:

  • The group have included quite a lot of information about fluorescent protein and some colourful photos.

Things to improve:

  • Some of them like, Gfp mouse1.JPG, Malaria.jpg and Prion coverting protein.jpg, have not been properly referenced.
  • At least one self- drawn diagram should be included.
  • The way of referencing is not consistent. More work should be done on correct citations.
  • The Links to Current Research and Glossary have not been completed.


Group 10 Project - Somatic Cell Nuclear Transfer

--z3269335 14:59, 11 May 2010 (UTC)

Good points:

  • Extensive researches are shown.
  • Most of the diagrams are correctly referenced with some descriptions.
  • It is a good idea the use of table to demostrate the advantages and disadvantages of SCNT.

Things to improve:

  • The layout of the diagrams could be improved or unified.


Lab 9 - Tissue Culture/ Microarray Visit

What is the technological bottleneck common to all next generation sequencing (NGS) platforms?

The technological bottleneck common to all next generation sequencing (NGS) platforms is the amount of time and resources required for template and library preparation.

--Rosita Pang 06:58, 12 May 2010 (UTC)

Lab 10- Discussion on how we can improve the project

Our group has discussed the comments given by our peers. The following is the summary of their suggestions:

  1. more images
  2. more diagrams to illustrate some methods
  3. more glossary terms (proto-oncogene, cDNA, FISH, microtome, perturbations)
  4. more tables
  5. more bullet points to replace long paragraphs
  6. references to current research

We agreed most of the above suggestions and would edit our project accordingly. Additionally, we would add more information to the timeline and we would create a session for helpful links. --Rosita Pang 07:45, 19 May 2010 (UTC)