From CellBiology

Individual page for z3254753

== Lab Attendance==--Mark Woods 10:51, 28 May 2011 (EST)

--z3254753 10:37, 10 March 2011 (EST)

--z3254753 09:18, 17 March 2011 (EST)

--z3254753 09:09, 24 March 2011 (EST)

--z3254753 09:26, 31 March 2011 (EST)

--z3254753 09:25, 7 April 2011 (EST)

--z3254753 09:12, 14 April 2011 (EST)

--z3254753 09:29, 21 April 2011 (EST)

--z3254753 09:21, 5 May 2011 (EST)

--z3254753 09:47, 12 May 2011 (EST)

--z3254753 09:15, 19 May 2011 (EST)

I was present at the lab on the 26/5, and was marked as attending on the role sheet. However I did not sign into a computer to sign my attendance on my student page --Mark Woods 10:51, 28 May 2011 (EST)

--z3254753 09:11, 2 June 2011 (EST)

Individual Assessments

Lab 1

1. What are the key cell biology journals?

The key cell biology journals are;

1. JCB (journal of Cell Biology)

2. Cell, Nature Cell Biology

3. Journal of Cell Science

4. Molecular Biology of the Cell

5. PNAS (Proceedings of the National Academy of Sciences of the United States of America.

2. Which journals allows users to reuse their material?

1. Journal of Cell Biology

2. BMC Cell Biology

3. Public Library of Science

Lab 2


1. Which chromosomes contribute to the nucleolus?

five chromosomes 13, 14, 15, 21, 22 contribute to the nucleolus.

--Mark Hill 08:01, 24 March 2011 (EST) Do you need help with this question?

2. Identify and add a link to your page of a recent cell biology article using confocal microscopy

Figure 9-19. Conventional and confocal fluorescence microscopy compared

Exercise: Look through editing basics, there is a link called project referncing,; step by step to a link to a reference in the Pubmed page.

Lab 3

1. Find the SDS information for chloroform and identify the hazards associated with this chemical.

Chloroform is a carcinogen. This means Absorption through the skin may result in exposure.

The United Nations Number (UN number)is 1888.

This substance had a A Chemical Abstract Service Registry Number (CAS Number: 67-66-3).

The classification of chloroform is

Xn; R22-48/20/22 Xi; R38 Carc. Cat. 3; R40


Xn : Harmful

R22-48/20/22 are risk phases associated with chloroform.

R22 is Harmful if swallowed.

R23 Toxic by inhalation.

R24 Toxic in contact with skin.

R25 Toxic if swallowed.

R48/20/22 Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed.

2. You will need to upload an image and add it to your page, with the reference and copyright information with the image.

FGF receptor expression pattern in a b3 stage lung.jpg




Lab 4

1. Identify a commercial supplier of an antibody that relates to your group project topic.

Two monoclonal antibodies, bd-17 and bd-24.


2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria? (Hint - Watch Part 2: Factors Involved in the Intrinsic Pathway of Apoptosis

Gene is located in the nucleus of the cell and is trapped in mitochondria by the mitochondria's outer membrane.

Lab 6

Group 3 graph.JPG

We noticed an increase in "stringed" phenotype and a significant decrease in "pronged" catagory when moving from over expressing Tm4 to control respectively. This shows that over expressing Tm4 increases strutural capabilities.

Work Area

Intro Lab


Lab 9

Identify from one of the cell line repositories: a neural cell line and a muscle cell line.


Homo sapiens (human)

Organ: brain Disease: neuroblastoma Derived from metastatic site: bone marrow


Rattus norvegicus (rat) Tissue: skeletal muscle Cell Type: myoblast myoblast

Identify the species and growth conditions for these cell lines.


Organ: brain Disease: neuroblastoma Derived from metastatic site: bone marrow

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°CStorage


Rattus norvegicus (rat) Tissue: skeletal muscle Cell Type: myoblast myoblast

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: The myoblastic component of this line will be depleted rapidly if the cells are allowed to become confluent.


lab 10

perr assessment note: assess as a uni student learning the material for the first time. is it clearly written are there glaring holes? are there repetitive parts? means they havnt collaborated point out gud points and the things that dont work so well. if identified specific effors mention that as well. do all images contain correct copyright info? a good assessment should fill half t he screen with a balance of positive and negative. done by next week

Peer Assessment

Group 2

The inclusion of very specific knowledge shows that the page is well researched and gives the right amount of detail to the reader. There are a variety of interesting pictures, from diagrams to photographs, all which add to the page. The pictures throughout the page need captions so there is a small amount of info about that picture actually with the picture on the page itself. The table of comparisons is a good idea, however I am not sure how much can be learnt about gap junctions themselves. It seems to take up too much of the page proportionally with a lot of information that seems best suited elsewhere. Despite this, the clear advantage of such a table is that it allows the page to not only be a resource for gap junctions, but as a starting point for learning about other types of junctions. This is very helpful for students looking for further knowledge not specific to the gap junctions themselves.

Group 3

This page is really informative and I found the intro with the accompanying picture immediately engaging. This page is complimented by several really helpful diagrams that allow the reader to easily picture tight junctions between cells, and how they perform their functions. Glossary should be a bit bigger,there are quite a few technical terms. The section molecular components is well written as there is not an overload of text. The other sections do contain more text which is overwhelming at first impression, but reading through seems reasonable given the content. Perhaps one more example under "Examples of Tight Junctions of the Body" would be beneficial, the section is great, but two examples perhaps doesn't show how big a role they play in the body. Overall, the page is a great resource for anyone looking into tight junctions

Group 4

Very well done. This is very technical and is very well research. High level of detail requiring specific knowledge that is not covered on the page. This page needs a glossary to explain terms, although it is meant for peers the technical nature of the page requires some explanation of terms to make the page more accessible. Not sure if it's worth a mention, but the pubmed references could be done differently (<rf><pubmd>PMID</pubmd><rf>, include the es)to give info about the resource on the page. The introduction is relevant but short, maybe including slightly more detail or theimportance of this junction would help. Besides these small details, I found this page incredibly informative with the amount of depth and detail, great work guys.

Group 5

Helful images both referenced and from the studnets compliment the written text. This page is well balanced with sensible headings, building comfortably on itself from the start to finish in terms of content. This is beneficial for the reader as he/she can easily learn from what is on the page, which fulfils this criteria to an extrermely high level. The table giving other types is a good idea, however, it does seem a bit extensive for something that is not on the topic. Conversely, it works well with it's iamges to provide a means of comparison between adherens junctions and other types. The inclusion of several external links throughout is great, as this page can now be used as a base for further study into the subject. If a section is dedicated to external links,then perhaps more than one should be listed. The history does seem a little short, both in number of dates and amount of detail for each major event.

Group 6

I was blown away with this project page. Not only is there a high level of detail, but it is presented in such a way that doesn't overload the reader.I particularly liked the EM section, it works well because of the balance of text to the pictures presented, and is very interesting. Perhaps the mechanisms of action should be numbered because it relays to a reader a sequence of events. Numbering what occurs would just reinforce this. Also, it could be handy to add some statistics to common nueromuscular junction disoders, to give an idea of how often these diseases occur. I know this seems like nit picking, but the page is so well done I could only suggest modifying some details. Well done.