User:Z3254433

From CellBiology

Lab Attendance

1. --z3254433 10:35, 10 March 2011 (EST)

2. --z3254433 09:33, 17 March 2011 (EST)

3. --z3254433 09:18, 24 March 2011 (EST)

4. --z3254433 09:37, 31 March 2011 (EST)

5. --z3254433 20:38, 7 April 2011 (EST)

6. --z3254433 09:16, 14 April 2011 (EST)

7. --z3254433 10:11, 21 April 2011 (EST)

9.lab 9 i was here but i forgot to sign on.

10. --z3254433 09:41, 12 May 2011 (EST)

11. absent doctors certificate given.

12. Lab 11. role marked in class.

13. --z3254433 10:20, 2 June 2011 (EST)

Individual Assessments

Lab 1

1. What are the key cell biology journals?

the journal of cell biology

public library of science

nature journals

trends in cell bioloy

cell


2. Which journal allows reuse of their published content?

Journal of Cell Biology, BMC Cell Biology and Public Library of Science allow reuse of content provided the article is properly cited.


here is some bold text

here is some italic text

internal link



Lab 2

[1]

Pubmed


lab questions

1. Which chromosomes contribute to the nucleolus?

chromosomes 13, 14, 15, 21, 22

2. Identify and add a link to your page of a recent cell biology article using confocal microscopy from the Pubmed database.

An Atlas for Schistosoma mansoni Organs and Life-Cycle Stages Using Cell Type-Specific Markers and Confocal Microscopy.

pubmed link




Lab 3

1.Find the SDS information for chloroform and identify the hazards associated with this chemical.

Potential Acute Health Effects: Hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, of inhalation. Slightly hazardous in case of skin contact (permeator).

Potential Chronic Health Effects: CARCINOGENIC EFFECTS: Classified + (Proven.) by NIOSH. Classified A3 (Proven for animal.) by ACGIH, 2B (Possible for human.) by IARC. Classified 2 (Some evidence.) by NTP. MUTAGENIC EFFECTS: Mutagenic for mammalian somatic cells. Mutagenic for bacteria and/or yeast. TERATOGENIC EFFECTS: Not available. DEVELOPMENTAL TOXICITY: Not available. The substance may be toxic to kidneys, liver, heart. Repeated or prolonged exposure to the substance can produce target organs damage.

Links

[http:/msds.chem.ox.ac.uk/CH/chloroform.html sds chloroform1] msds chloroform

2.You ll need to upload an image and add it to your page, with the reference and copyright information with the following.


Red White Blood cells.jpg [1]

A three-dimensional ultrastructural image analysis of a T-lymphocyte (right), a platelet (center) and a red blood cell (left), using a Hitachi S-570 scanning electron microscope (SEM) equipped with a GW Backscatter Detector.

Image Reference

  1. http://en.wikipedia.org/wiki/File:Red_White_Blood_cells.jpg



Lab 4

1. Identify a commercial supplier of an antibody that relates to your group project topic.

biocompare can provide:

  • Mouse Anti- Human ZO1 tight junction protein Antibody, Unconjugated, Monoclonal, Clone mAbcam 61357
  • Rabbit Anti- Human ZO1 tight junction protein Antibody, Unconjugated, Polyclonal

biocomapre


2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria? (Hint - Watch Part 2: Factors Involved in the Intrinsic Pathway of Apoptosis

  • the CYCS gene that encodes cytochrome C is located in chromosome 7.
  • the protein cytochrome C is keep trapped by the outer mitochondrial membrane.



Lab 6

Lab 6 table.JPG

Q 1) what are the changes in phenotypes that you observe between group A and group B?

A =Fan B = Broken Fan C = Stumped D =Pronged E = Stringed F = pyogenic Group a seems to have a greater number of phenotype C and D.


Q 2) how does Tm4 mediate these changes?

The over expression of Tm4 mediates the growth of the neurites.

other observations with regard to morphology?

An increase in the pronged and stringed phenotypes.


Lab 9

1. Identify from one of the cell line repositories: a neural cell line and a muscle cell line. neural cell line:

HUV-EC-C

muscle cell line: G-8


2. Identify the species and growth conditions for these cell lines.

neural cell line: species: Homo sapiens (human)

growth conditions;

1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37°C.

muscle cell: species; Mus musculus (mouse)

growth condition;

Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks.Differentiation is enhanced by reducing the sera to 2% each.



Peer review

Group 1

  • I like the fact that you give a brief overview of the nervous system in the introduction, so that the reader better understand your topic.
  • I like the history section as it is not too long, but informative, but maybe you can add some newer finding in this section.
  • Don’t forget to take out your name of the section you completed.
  • In the neurotransmitter section second sentence just fix the word chemical s, where there is an extra space between the L and S. So it will be a good idea to go over the whole assignment again and check grammar and spelling.
  • Good use of a table to explain the types to neurotransmitters.
  • The disease section is a bit long compared to the rest of the assignment, so there is probably some information missing in the other sections.
  • Your glossary would be easier to read if it was in alphabetical order.
  • Finally the gallery should be in the discussion page.


Group 2

  • just to start of with i just wanted to mention that you do not have a title saying gap junction on your page.

references:

  • your first picture called specialized cell junction, does not have a reference.
  • All the images are relevant but not all the images have a description beneath it which would be much more helpful.
  • I like the way you broke down the locations of gap junctions and possible diseases, which made it easier to read.
  • the glossary section could be longer. It would also be easier to read if the glossary is in alphabetical order.

Group 3

  • I like the introduction as you explain the topic of junctions overall, as well as yours.
  • I like the timeline as it covers a wide range of dates, but is still nice and short.
  • I liked the further explanation on the proteins that make up tight junctions.
  • You need to lengthen your glossary list.
  • I think that the disease section is too long compared to the rest of the sections in your assignment.
  • Some of the images do not have a reference as they may be from our cell biology wed site or wiki, I’m not sure if you have to but you should still say where you got the image from.
  • Overall a really good assignment.


Group 4

  • Good concise introduction
  • Well detailed history, maybe something current should be included in this section.
  • Possibly some more information on the current research.
  • The subheadings under "structure" need some fixing as the whole structure under that section is a bit too long and complex, this makes it hard to follow the point you are trying to get across.
  • A glossary should be included, as this is an open access site and the content should be understood by all who would like to read it.
  • Do not forget to check spelling and punctuation, for example you have the word (cells’) written as so in the extra cellular domain subheading, 5th paragraph.
  • Overall it was very informative to read this assignment.


Group 6

  • A well written introduction.
  • I like the short history section, but maybe add 1 or 2 more recent points.
  • I liked the use of images, videos, tables and links.
  • There are too many pictures close to each other in the future research section, so just some layout issues to be fixed.
  • Other than that a VERY well done assignment, it was very informative and creative. Good job guys.