From CellBiology

Lab Attendance

--Paul Lee 10:56, 10 March 2011 (EST)

--Paul Lee 10:42, 17 March 2011 (EST)

--Paul Lee 09:44, 24 March 2011 (EST)

--Paul Lee present, 31 March 2011

--Paul Lee 09:13, 7 April 2011 (EST)

--Paul Lee 09:11, 14 April 2011 (EST)

--Paul Lee 09:42, 21 April 2011 (EST)

--Paul Lee 10:48, 5 May 2011 (EST)

--Paul Lee 10:07, 12 May 2011 (EST)

--Paul Lee 09:28, 19 May 2011 (EST)

--Paul Lee 09:37, 26 May 2011 (EST)

Work Space

Lab 2


Lab 3

This is about frog nucleoli shape changes.[1][2]

Individual Assesment

Lab 1

Q1. what are the key cell biology journals?

A:cell biology journals

  1. Cell
  2. Nature: Cell biology
  3. Journal of Cell Biology
  4. BMC Cell Biology
  5. Public Library of Science

Q2. Which journals allow reuse of their published content?

A:Cell Biology Journals that allow reuse of their published content

  1. Public Library of Science
  2. Journal of Cell Biology
  3. BMC Cell Biology

Lab 2

Q1 Which chromosomes contribute to the nucleolus?

A: The nucleolus, located within the nucleus, functions to house the transcription and assembly of rRNA. Within the human body, the chromosomes which contribute to this activity are - 13,14,15,21,22 chromosomes.[1]

Q2 Identify and add a link to your page of a recent cell biology article using confocal microscopy

A:Isolation, characterization and neural differentiation potential of amnion derived mesenchymal stem cells.

Lab 3

Q1 Find the SDS information for chloroform and identify the hazards associated with this chemical.


Health hazard information of Chloroform

1. Harmful if Swallowed.

2.Causes Skin Irritation.

3.Causes Eye Irritation.

4.Suspected of causing cancer.

5.Suspected of Damaging the unborn child.

6.May Cause Drowsiness or Dizziness.

7.May Cause Damage to Liver and Kidneys Through Prolonged or Repeated Exposure.

Occidental Chemical Corporation Safety Data sheet

Q2 You will need to upload an image and add it to your page, with the reference and copyright information with the image.

A: Non-invaded first trimester decidua.jpg

non-invaded first trimester decidua[3]

Copyright Nikitina et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

Lab 4

Q1. Identify a commercial supplier of an antibody that relates to your group project topic.

A: Abcam is a supplier of a variety of antibodies. To name one specifically, the antibody (ab59720) is a rabbit poly-clonal to the ZO1 tight junction. This antibody is able to bind to the ZO1 tight junction protein on humans, mice and dogs.[2]

Q2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria?

A: Cytochrome C is a protein localised in the mitochondria of a cell and takes part in the electron transfer chain as well as being involved in the initiation of apoptosis. It is encoded by the CYCS gene located on chromosome 7 (Video). Due to the selectively impermeable nature of the inner membrane, Cytochrome C is trapped within the inner membrane of mitochondria and is also, in a sense, trapped by the outer membrane of the mitochondria.

Lab 6

Percentage Comparison Of morphological phenotypes in Tropomyosin 4 over-expressing B35 neuro-epithelial cells.JPG

Percentage Comparison Of morphological phenotypes in Tropomyosin 4 over-expressing B35 neuro-epithelial cells (Group 1)

Group A: Tm4 Group B: Control

Phenotype: A - Fan, B - Broken Fan, C - Stumped, D - Pronged, E - Stringed, F - Pygnotic


Part A (Group 1)

1. What are the changes in Phenotypes that you observe between group A and group B? For group A, the more frequent phenotype tended to be localized towards the pronged, stringed phenotypes. However, for group B, they tended to be more towards the Fan, Broken fan and stumped phenotype.

2. How does Tm4 mediate their changes? Understanding that Tm4 was overexpressed within group A, we could see through these observations that Tm4 clearly plays a role in phenotypic differentiation. As opposed to group B, as group A displayed a greater frequency of the type D and E phenotypes, it could be deduced that the overexpression of Tm4 in group A created an increase in the growth of processes of the dendritic cell. Conversely, lacking the overexpression of the Tm4 caused the formation of shorter processes leading to phenotypes A-C.

Part B (db cAMP induced differentiation of B35 cells)

1. What are the changes in Phenotypes that you observe between group A and group B? Similar to the group 1 observations, Group A expressed a greater frequency for the Type D and E phenotype (longer processes), while group B expressed group A-C (shorter processes). Furthermore, Group A showed greater branching in their neurites.

2. How does Tm4 mediate their changes? Tropomyosin is an actin binding protein and accounts for the stability of actin filaments through the regulation of actin assembly and organisation. It has been discovered that there were high concentrations of Tm4 in the neurites of young animals [4]. This may point to the notion that Tm4 is associated with neurite growth. This paralleled our observations in that the cells with overexpressed Tm4 showed greater length of neurite growth and branching. Thus, we can assume that with Tm4, it allows for greater process growth and branching, which are essential for the development of young animals.

Critique of Project Pages

Group 1

Intro - It would be better if the introduction is broken up for easier read

history - Great!

What is SJ? - The elaboration of the components of the SJ would allow for better understanding

Type of SJ - Outlining where they are located and their actions in those locations would be good

modulation - What is the effect of modulation?

neurotransmitters - Good use of tables! allows for easy read

Diseases - good! the layout is good however making the titles of the disease more distinct would be good. If you quickly scroll down on this section you dont really know what disease youre talking about.

Current and future research - More potential research studies would be good

Glossary - Alphabetically rearrange

Overall, good site just need to elaborate on some of the points you mentioned. Good work!

Group 2

Good work group 2. It was a good read but is it really necessary to discuss the other junctional structures in that depth? Also there are alot of points you could elaborate on such as:

- structure of the gap junction (this section seems too brief)

-The functional role (the 4 points you mentioned i.e. 1) Transmission of excitation in cardiac muscle, smooth muscle and central nervous system (CNS) neurons...etc)

also, for the diseases section, it would be better if you could make the headings for the types of diseases bigger so that people wouldnt get confused.

Otherwise, an overall good page! :)

Group 4

Good work group 4. It is a very detailed yet concise page! However for the introduction and the function of this junction needs abit of fleshing out. The introduction seems abit to brief. Maybe the brief mention of what you will be talking about throughout the page or even just going into abit more detail to get the readers interested in this topic. For the function section, use of examples would be better to allow the readers to have a better grasp of what you are talking about. I like your groups use of pictures, they go really well with the text. Good work guys!

Group 5

Hey guys, you have an awesome page! However I have a few things that you could improve on. The history seems abit too brief. A few more dates and key figures that led up to not just the discovery of this junction but rather the function or their role in diseases would be good. Furthermore, for the disease section, the use of bigger headings for the title of the diseases would be better for the readers. Other than that, it is an awesome page :)

p.s. Is it really necessary to discuss the other type of junctions in that much detail?

Group 6

Good work guys! I really liked the video to describe the process. It really allows for a good understanding of the process! However for that section, rather than using the dotpoints, wouldn’t it be better if you used a numbering system (to show the progression of the mechanism of action?) Also I think it would be good to discuss how muscle contraction occurs (i.e. following activation of the motor neuron, the signal transduction pathway that activates the muscle and causes the contraction). Otherwise, a job well done! :)

Reference list

  1. Clifford P Brangwynne, Timothy J Mitchison, Anthony A Hyman Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes. Proc. Natl. Acad. Sci. U.S.A.: 2011, 108(11);4334-9 PubMed 21368180
  2. Duncan Sproul, Colm Nestor, Jayne Culley, Jacqueline H Dickson, J Michael Dixon, David J Harrison, Richard R Meehan, Andrew H Sims, Bernard H Ramsahoye Transcriptionally repressed genes become aberrantly methylated and distinguish tumors of different lineages in breast cancer. Proc. Natl. Acad. Sci. U.S.A.: 2011, 108(11);4364-9 PubMed 21368160
  3. Liudmila Nikitina, Helmut Ahammer, Astrid Blaschitz, Angela Gismondi, Andreas Glasner, Michael G Schimek, Gottfried Dohr, Peter Sedlmayr A new method for morphometric analysis of tissue distribution of mobile cells in relation to immobile tissue structures. PLoS ONE: 2011, 6(3);e15086 PubMed 21408197
  4. L Had, C Faivre-Sarrailh, C Legrand, J Méry, J Brugidou, A Rabié Tropomyosin isoforms in rat neurons: the different developmental profiles and distributions of TM-4 and TMBr-3 are consistent with different functions. J. Cell. Sci.: 1994, 107 ( Pt 10);2961-73 PubMed 7876361