User:Z3251370

From CellBiology

Lab 1 Q:What Cell Biology Journals allow reuse of acknowledged and correctly cited content?

The journals that allow students/users use, distribute, reproduce, copy and display the content as long as the article is properly sited are:

1- The journals, published by "Journal of Cell Biology"

 - Journals in the Homepage and content in the Archives 

2- The journals, published by " BMC Cell Biology"

 - Journal Archive from 2000 to 2009 can be used.

3- The journals, published by Public Library of Science

 - PLoS Biology
 - PLoS One
 - PloS Medicine 

The user must ask for permission to use the content of these Journals:

1- Nature Journals

 - Nature Cell Biology
 - Nature Milestones in Cytoskeleton 
 - Nature Reviews Molecular Cell Biology

2- Trends in Cell Biology

3- Current Opinion in Cell Biology

4- Cell

5- Journal of Cell Science

6- Molecular Biology of the Cell

7- Proceedings of the National Academy of Sciences

--Ozgur Tuna 23:31, 16 March 2010 (UTC)


Lab 2 What are the "light" regions of DNA within the nucleus called?

The lighter areas inthe nucleus are called euchromatin. The lighter staining is because of the less compact structure of euchromatin.


--Ozgur Tuna 05:54, 17 March 2010 (UTC)

Lab 3 - How do we know the hazards associated with research chemicals?


Material Safety Data Sheets (MSDS) are prepared for each of the chemicals used within the laboratory.

Before the techniques are carried out the uses and location of MSDSs should be shown.

This should include:

The hazards, risk that might occur during the use of specific chemicals, labelling, storage, handling and disposal.


MSDS must state:

The product name of a hazardous substance Precautions for safe use and handling Information for health hazard Hazardous substance's physical and chemical properties. The chemical and generic name of certain ingredients The manufacturer's or importer's name, Australian address and telephone number.

--Ozgur Tuna 06:53, 24 March 2010 (UTC)


Lab 4 - Identify a commercially available antibody to an adhesion junction protein, and also add a link to the antibody page on your own student page


p120 Catenin (C-term), Mouse Monoclonal Antibody

This antibody is specific for p120ctn proteins and can be used to detect isoforms (1-4) of the protein.It is reactive with mouse, rat, dog, chicken and human p120ctn. Although the reactivity with chicken protein is weak, it is still detectable. Immunofluorescence, Western blottong and immunoprecipitation techniques are used to confirm the reactivity of the antibody.

Anti p120ctn binds directly to the juxtamembrane region of cadherins. They have important role in the regulation of cadherin mediated adhesion and also function in nuclear signalling and cell-cell adhesion.

Link

http://products.invitrogen.com/ivgn/product/339600

--Ozgur Tuna 04:16, 14 April 2010 (UTC)


Lab 5

Knockout methods handouts were completed and given to the pHD student in the lab.

--Ozgur Tuna 04:48, 2 June 2010 (UTC)


Lab 6


Z3251370.JPG

Lab 7 Identify an advantage to using each of the following objectives: Air, Water or Oil


Oil: The resolution is highest when oil is used. Specimens must be fixed.

Air: It is possible to observe live specimens and not there is no mess occuring.

Water: Higher resoultion viewing than when air is used. Live specimens can be observed.

--Ozgur Tuna 04:48, 2 June 2010 (UTC)


Lab 8


PEER REVIEW

Headline text

Group 1 Fluorescent-PCR

Good effort guys! Good organization of the page and very useful information about PCR.

Here are some points to consider for improvement:

  • Glossary is missing
  • Some pictures are small and writings are blurry, not easy to read.
  • More references can be good. References section is too short.
  • Maybe a table can be used for better, easier understanding in the Comparison section when talking about advantages provided by F-PCR compared to C-PCR
  • A few mistakes in grammar.

Group 2 RNA Interference

Well done guys, very good work! Into and History are great, not boringly long. Excellent student drawn diagram which is explaining the process very good. Using a table in advantages – disadvantages section is a great way to point out the differences. Applications section is very clear, informative and well organized as applications in different areas in Medicine are explained well.

Some points to improve :

  • Glossary can be reorganized, though. It looks like some information (definitions) is missing in glossary.
  • Referencing the pictures is necessary.

Great overall work!

Group 3 Immunohistochemistry

Good job guys! Very good flow. Tables made it easy to understand in each method.

Here are my advices in a few points:

  • Glossary can be reorganized as not everybody reading this page will be experts.
  • The picture in Avidin-Biotin Complex Method (ABC Method) was not referenced.
  • Many methods were explained with clear tables. That was great but maybe comparison between the methods can be added too. Each time I read a different method, I had to check the previous methods again to see the differences. For example, Direct method involves using primary antibodies where as Indirect method involves using both primary and secondary antibodies.
  • The student drawn picture is hard to understand in the project page. I t can be a bit bigger.
  • In the process section, there are no references.

Group 4 Cell Culture

Good effort guys, great page on Cell Culture. It is well written with good structure. Applications and Limitations (with bullet points) are very well explained. Overall, very good page!

A few points to improve:

  • Student drawing can be made bigger.
  • Starting from the Applications section, no references are available. Throughout the page, references must be worked on more.
  • Timeline can be added.

Group 5 Electron Microsopy

Good work guys, your page has a nice layout and it is nice to see there is a great use of pictures with good format and referencing. Comparison between TEM and SEM by using a table is a great idea. Also the Uses at UNSW and section is a great touch as it is nice to see the applications in UNSW.

A few points to improve:

  • Glossary can be added as readers will need definitions for better understanding.
  • History section can be relocated and placed just after Intro.

Group 6 Confocal Microscopy

Great work Guys!!! The first thing that hit me was the Student Drawn Diagram!!! Also well presented information and references about Connfocal Microscopy.

My suggestions:

  • The Glossary in Content section grew tooooo long.
  • Introduction is missing.
  • Timeline can be reorganized to show the significant developments in a more clear way, maybe by using bullet points.

Group 7 Monoclonal Antibodies

Good work guys! Your page is well organized and very informative about monoclonal antibodies. It is obvious how much research you have made. Page has a very good use of images. Good overall work!

My suggestions:

  • The way you showed References must be fixed. You have a long References section but there is no link between the articles and where they belong throughout the page.
  • Glossary can be a bit longer with more definitions of terms.
  • Use of video is a good idea.
  • History section is too much detailed and long.

Group 8 Microarray

Good work guys. Your page is very well structured. The use of image showing the comparison between one colour and two colour expression analysis is great, makes it easy to understand.

My suggestions:

  • Definition and use Section can be added into the Introduction
  • History Section is a bit short. Is there no significant event/development since the 90’s? References for current research and development in Microarray would be great.

- A few more definitions can be added into Glossary (like cDNA…etc)

Group 9 Fluorescent Proteins

Great page guys! You have a very informative page. It shows how much research you have made.

My suggestions:

  • Structure and Classification section can be shown under a different title ( not in History section)
  • I could not see a student drawn image. - Use of tables and images can be helpful to break up the text.
  • Glossary must be reorganized and definitions must be added.
  • References must be fixed as readers should be able to go into the reference while reading.

--Ozgur Tuna 05:02, 12 May 2010 (UTC)

Lab 9

I could not attend the lab 8 as I had an appointment to see a GP.

--Ozgur Tuna 04:48, 2 June 2010 (UTC)

Lab 10

I attended the lab

--Ozgur Tuna 04:48, 2 June 2010 (UTC)

Lab 11

I attended the lab and handout was completed and given to the deomstrators in the lab.

--Ozgur Tuna 04:48, 2 June 2010 (UTC)