User:Z3213957

From CellBiology

Lab 1 - What Cell Biology Journals allow reuse of acknowledged and correctly cited content?

The Journal of Cell Biology, BMC Cell Biology, Public Library of Science

--Jae Choi 06:46, 10 March 2010 (UTC)


Lab 2 - What are the "light" regions of DNA within the nucleus called?

Euchromatin

http://www.cytochemistry.net/Cell-biology/Nucleus.htm

--Jae Choi 07:04, 17 March 2010 (UTC)


Lab 3 - How do we know the hazards associated with research chemicals?

We can find Material Safety Data Sheets (MSDS) which is a set of standardised safety information prepared for each of specific chemicals used within the laboratory.

It states the product name, the chemical and physical properties, health hazard information, precautions for safe use and contact details of the manufacturer.

UNSW Human Resources also provides a website called UNSW Chem Alert which shows a detailed list of hazadous chemicals used within UNSW laboratories.

--Jae Choi 07:01, 24 March 2010 (UTC)


Lab 4 - Identify a commercially available antibody to an adhesion junction protein, and also add a link to the antibody page on your own student page.

connexin 26 (O-24)

-Primary antibody

-Rabbit polyclonal IgG

-It is used for detection of mouse and human connexin 26

http://www.scbt.com/datasheet-130729-connexin-26-o-24-antibody.html

--Jae Choi 06:16, 31 March 2010 (UTC)


Lab 6

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--Jae Choi 07:59, 21 April 2010 (UTC) here today


Lab 7 - From today's confocal tutorial, identify an advantage to using each of the following objectives: Air, Water or Oil.

Air: By using this objective it can minimise haziness and no clean up needed. But the specimen can get dried then cause damages.

Water: Can reduce spherical aberration and it allows to observe living cells especially aqueous cells and/or tissues. Therefore, the normal function of the cells and tissues can be observed.

Oil: It is capable to observe higher resolution of the microscopic image at high magnification (x100). However, the specimen must be fixed before using this objective.

--Jae Choi 03:19, 5 May 2010 (UTC)


Lab 8 - Your assessment item is to critically review all other group projects. Use the Group Assessment Criteria as discussed in the lab. Record your peer assessments on each group talk page, with your signature. Add the project title and a brief comment to your own page.

Reviewed Group 10 project "Somatic Cell Nuclear Transfer" - Well organised structure, clearly described, lots of interesting pictures including a links to video. “Advantages and Disadvantages” part is relatively too short and less likely explained and no references are provided for the last two points of “Advantage” part. --Jae Choi 11:37, 5 May 2010 (UTC)


Reviewed Group 9 project "Fluorescent Proteins" - project still needs to be organised. Wiki referencing style required. --Jae Choi 13:48, 10 May 2010 (UTC)


Reviewed Group 8 project "Microarray" - Well organised, clearly described, lots of interesting pictures including a link to video. Some more for the Glossary part would be better. --Jae Choi 13:49, 10 May 2010 (UTC)


Reviewed Group 7 project "Monoclonal Antibodies" - Too long history part (too much in detail) and no glossary part has been provided. No wiki referencing system is used. --Jae Choi 13:50, 10 May 2010 (UTC)


Reviewed Group 6 project "Confocal Microscopy" - No introduction has been provided at the beginning of the page. Unnecessary subheadings at the Glossary part. --Jae Choi 13:51, 10 May 2010 (UTC)


Reviewed Group 5 project "Electron Microsopy" - The part Uses at UNSW (briliant). No references for the simplified schematic drawing of transmission electron microscope and another one for scanning electron microscope. --Jae Choi 13:52, 10 May 2010 (UTC)


Reviewed Group 4 project "Cell Culture" - Introducing only the main scientists for the History part. It would be better if timeline or some pictures are added. --Jae Choi 13:52, 10 May 2010 (UTC)


Reviewed Group 3 project "Immunohistochemistry" - Impressive methods part with the simplified drawings and Advantages & Disadvantages, each method is clearly described. Incomplete Glossary part. No references for the process part. --Jae Choi 13:53, 10 May 2010 (UTC)


Reviewed Group 1 project "Fluorescent-PCR" - No Glossary part provided. Not enough references. --Jae Choi 13:54, 10 May 2010 (UTC)


Lab 9 - Microarray Lab: What is the technological bottleneck common to all next generation sequencing (NGS) platforms?

The bottlenecks for next-generation sequencing is the amount of time and resources required for template and library preparation. The current library preparation methods for next-generation sequencing are time-consuming and prone to considerable sample loss. [1]

--Jae Choi 07:00, 7 July 2010 (UTC)