User:Z3126328

From CellBiology

--Jin Lee 05:39, 10 March 2010 (UTC)



Lab 1What Cell Biology Journals allow reuse of acknowledged and correctly cited content? The Journal of Cell Biology, BMC Cell Biology and Public Library of Science allow reuse of content provided the article is properly cited. --Jin Lee 03:52, 17 March 2010 (UTC)


Lab2 What are the "light" regions of DNA within the nucleus called? The "light" regions of DNA within the nucleus is Euchromatin. It is most abundant in active, transcribing cells.(Alberts et al, Molecular Biology of the Cell, Garland Publishing, 1994, pp 351-352). Thus, the presence of euchromatin is significant because the regions of DNA to be transcribed or duplicated must uncoil before the genetic code can be read.

just to be noted, the "darker" region or condensed form is Heterochromatin. Abundant heterochromatin is seen in resting, or reserve cells such as small lymphocytes (memory cells) waiting for exposure to a foreign antigen.

A microscopic view of chromosome organization is available --> [www.cytochemistry.net/Cell-biology/nucleus.htm]

--Jin Lee 05:27, 24 March 2010 (UTC)


Lab 3 How do we know the hazards associated with research chemicals?

Material Safety Data Sheets (MSDS) are a set of standardised safety information prepared for each of the chemicals used within the laboratory. Each research laboratory is required to keep either a hardcopy or electronic copy of these MSDS's available within the laboratory. Before carrying out any new research technique, in particular for students, should be taken through the location and use of MSDSs. the risks and hazards involved with specific chemicals. the correct storage, handling, labeling and disposal of each chemical. ideally they should keep an electronic copy or link to each of these MSDS's for their own reference. --Jin Lee 06:46, 24 March 2010 (UTC)


Lab 4 Identify an commercially-available antibody against an adhesion protein and also add a link to the antibody page on your own student Murine monoclonal anti-VAP-1 IgM antibody vepalimomab binds to the binding to the extracellular domain of the vascular adhesion protein (VAP-1). http://www3.interscience.wiley.com/cgi-bin/fulltext/118719049/PDFSTART --Jin Lee 07:44, 1 April 2010 (UTC)


Lab6 Tm4


Lab7 From today's confocal tutorial, identify an advantage to using each of the following objectives: Air, Water or Oil.

  • Air: Easy to prepare,minimum equipment required. But they have minimum details.
  • Water: Allows visualisation of live cells since they are placed in their natural liquid medium.
  • Oil: provide best visualisation of cells as they removes any particles from air or dust between objective and specimen, however the oil preparation means the cells/organisms have to be dead/fixed so no real-time processes.

Lab8 --Jin Lee 07:08, 5 May 2010 (UTC)

Peer Review Group1: DEVELOPMENT OF PCR 4th paragraph,'In the early 1990s, anchored PCR was developed by Gail Martin and Mark Davis of Stanford. This method overcomes the limitation in the basic PCR. ~~' Can you link reference? with Principles of Fluorescent-PCR Procedures part,Great work! one suggestion is include student-drawn diagram? and with images, I can't not see any links or copyright permission.Also it will be good if you can briefly describe about the image. with reference part, I think it's kind of too short according to the all the information you have written in the wiki page. Also, adding Glossary section will help to understand any unsure words


Lab9 Microarray Lab: What is the technological bottleneck common to all next generation sequencing (NGS) platforms?

The bottlenecks for next-generation sequencing is the amount of time and resources required for template and library preparation. The current library preparation methods for next-generation sequencing are time-consuming and prone to considerable sample loss.