Talk:Protein - Caspase 3

From CellBiology


The cleaved-Caspase-3 antibody is a marker of Caspase-9-like DRONC activity in Drosophila

Cell Death Differ. 2010 Mar;17(3):534-9. Epub 2009 Dec 4.

Fan Y, Bergmann A. Source Department of Biochemistry and Molecular Biology, Graduate Program in Genes and Development, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard-Unit 1000, Houston, TX 77030, USA. Abstract The cleaved-Caspase-3 antibody is a popular tool in apoptosis research in Drosophila. As the antibody was raised against cleaved human Caspase-3, it was assumed that it detects cleaved DRICE and DCP-1, Caspase-3-like effector caspases in Drosophila. However, as shown here, strong immunoreactivity persists in apoptotic models doubly mutant for drICE and dcp-1. In contrast, mutants of the apoptosome components DRONC (Caspase-9-like) and ARK (Apaf-1 related) do not label with the cleaved-Caspase-3 antibody. By peptide blocking experiments and further genetic studies, we provide evidence that the cleaved-Caspase-3 antibody recognizes multiple proteins including DCP-1 and likely DRICE, but also at least one additional unknown protein, all of which require DRONC for epitope exposure. The unknown substrate may be involved in non-apoptotic functions of DRONC. Because the cleaved-Caspase-3 antibody not only detects cleaved Caspase-3-like proteins in Drosophila, but also other proteins in a DRONC-dependent manner, it is more accurate to consider the cleaved-Caspase-3 antibody as a marker for DRONC activity, rather than effector caspase activity.

PMID 19960024