Talk:3222840

From CellBiology

Peer Assesment Section

Peer reviewing : 3132868, 3158969, 3126345, 3189168, 3187155, 3201742, 2209471, 3186815, 3186582, 3161979.

A)In the structure part of the project, I think it needs to be focused specifically on structure of the protein. Structure part was bit lengthy and it made distracted. Those below copies are from the structure part, it would be better if these were moved to the clinical part and mechanisms part accordingly.

The disruption of this ubiquitous structures can cause acute promyelocytic leukemia (APL) and in DNA virus infection whereby the virus encode a protein and distrupt the NBs. SP100 protein and PML are covalently modified by the small ubiquitin-related protein SUMO-1. The modification requires nuclear localisation signal (NLS) and involves some lysine residue.

SP100 is also known to bind and interact with the members of heterochromatin protein 1 (HP1) families of non-histone chromosomal proteins. This protein present targets for the process of transcription, DNA replication and repair


B) z3280894- I agree that your project is lengthy but I think if you were to create more subheadings rather than just the two it would seem more broken up and easier to read. Maybe create a function subheading and also a glossary. Also, check to see if your pictures are labeled correctly with copyright information.

*Addition of subheadings are done. The copyright informations are provided when you click on the pictures, and it will direct you into the main page of the original uploaded page.

c) I agree with the above... perhaps some more information about the function and dynamics of the protein... also, paragraph 1 of the introduction contains a fragamented sentence:

SP100 is part of the component in the nuclear body that may involve during the immune response by regulating the transcription process

* Some information about the function has been added. The sentence is now broken into smaller fragments.


D) z3132868: You might want to put the history part in the introduction as to what people have discovered about this protein in the past, and then conclude the project by providing some current research and further research. The big chunks of words made it quite difficult to follow and understand, you might want to break it down into subheadings.

It shouldnt take too much to make this into a presentable body of work since you've obviously dont the readings. --Peter Kehoe 12:44, 21 May 2009 (EST)

*The history part is now moved into the beginning.

E) You might like to consider the following suggestions. Firstly, reconstruct your page. ie. Relocate history under introduction. Secondly, reorganise subheadings. ie."Overview of SP 100, Historical background of SP100, Structure and Function of SP100, blah, blah". Thirdly, focusing on functional aspect of the SP100, such as function of SP100, current research on SP100. Fourthly, you might like to devote a section to link your individual topic to group topic. eg."SP100 is such a cool protein, because it plays an essential in nucleus, blah, blah..". One more thing, condense your materials, just to make it easy to read. Overall, a good page

*Subheadings Overview, Structure and Function have been added

F) Whats been said above pretty much covers everything I've got to say. Maybe put your homework on this page so that it doesn't clutter up your assignment page. Good page.

*Homework section has been moved into discussion page.

G)Good information on SP100. Perhaps introduction could be broader and more said on function and current research.

  • a current research from 2007 might be the latest relevant information that can be provided.*

H) I don't think there is much for me to say that hasn't been said already, but i'll try to make a summary of the above and my own thoughts.

  • project structure:maybe use more subheading to separate the contents to allow easier reading.
  • perhaps consider adding more information the functions of SP100, as there is so much information the understanding of its function tends to be lost. -break it down a little more.
  • like most have said above you can make the page a bit more current in terms of adding a section for current research , it is a lengthy page so don't add to much but a few sentences should be great.

I) I agree with the above comments, so I won't repeat whats already been said. However, I do suggest putting a picture that illustrates the structure of the protein because all the acronyms do make it quite confusing. Also, putting a "function" heading would help break some of the information up and make the layout a bit clearer and give you an opportunity to put a link to your group page. Good referencing throughout. Overall, good stuff!

*Pictures of the protein structure is now added

J) on the whole i think you've done a great job and you should do very well, the content is great, its just a matter of making it clearer of the readers to follow and understand by breaking up the blocks of informaton. well done(3191801)

K) Overall looks and reads quite nicely. However, i feel as though there is a bit of information lacking. Maybe put the history in the introduction and shorten it, so you can expand on more research.

L) Overall from what has been presented I thought it was well done. With the addition of more pictures and information on other aspects of SP100 with the same amount of depth I think would make for a concise and informative page.

* I have added some more pictures into the page.


Other comments

--Mark Hill 10:00, 20 April 2009 (EST) You have not yet selected your individual project protein/method topic and still missing some homework items. 2009_Student#Individual_Projects Individual Projects

--Mark Hill 18:35, 19 March 2009 (EST)You still need to provide feedback for Lecture 4 - Nucleus.

--Mark Hill 00:14, 31 March 2009 (EST) Thank you for your feedback on the Nucleus lecture, "It is one of the main component of eukaryotic cell", not a very insightful comment about the nucleus. I was after something you may not have known about the nucleus or found interesting.

--Mark Hill 22:39, 31 March 2009 (EST) Nucleus information is much better now. You have made the effort to think about the lecture content, well done. Though I don't know what "and regulate the whole body's function respectively." means exactly? Now you just need to add the other homework topics. Homework

--Mark Hill 10:38, 3 April 2009 (EST) Your energy answer is OK, I was more after specific cellular processes (like intracellular transport) so that you would see that mitochondria locate where energy consumption is highest. You still need to answer the other homework questions. 2009_Student#Individual_Projects


HOMEWORK

Nucleus

Nucleus is one of the main components and only present in eukaryotic cell. It conducts DNA trascription to mRNA and mRNA need to be exported to cytoplasma to undergo translation to protein.

Nucleus has a double layer membranes that is connected to Endoplasmic Reticulum continuously. Cytoskeleton is also very crucial in order to hold the shape and structure of nucleus. The surface contains nuclear pores whereby passive transport of small ions and active transport of macromolecules can pass through.

Nuclear bodies contain specific compartments such as Cajal Bodies and PML bodies which fuctioning as modificator of small proteins and regulate the whole body's function respectively.

Exocytosis

The concept whereby post-Golgi carriers have different ways of conducting them. What are the advantages of having different pathways and how do you know which cargos will get particular way of behaviour during post-Golgi carriers?

Mitochondria

What types of cellular processes require lots of energy from the mitochondria?

Complex animal cells need to retain energy from mitochondria through aerobic respiration. Mitochondria produce 15 times more ATP than anaerobic process which is required by the cells. The initial source is from glucose whereby it is processed and imported into mitochondria and undergo catabolism process such as Kerbs cycle, fatty acid oxidation, and amino acid oxidation.

Other examples are cell signalling, cell reproduction such as mitosis and meiosis.


Cell adhesion molecules (CAM)

Ng-CAM: Neuroglia Cell Adhesion Molecule

I-CAM: Intercellular Cellular Adhesion Molecule

L-CAM: Liver Cell Adhesion Molecule


Intermediate Filament

What is the name of the epidermal layer between the basal and granulosa layer and how does it relate to intermediate filaments?

The tissue layer is called stratum spinosum which intermediate filaments relate to.

Phenotypes lab

The differences between Genotype A (Tm4 over-expressing B35 cells) and genotype B (wild type/lack of expression as control B35 cells) were :

  • A has shown to produce more neurites and lamella with more prominent processes. The count can reach up to 6 processes.
  • Genotype B was seen to have relatively smaller diameter.

Confocal Microscopy

Two major types of Confocal microscope generate its signal are by:

  • Laser Systems for Confocal Microscopy

The source is from fluorophores whereby the biological molecules reach the excited state and emit ultraviolet or visible light photons and captures them to produce the image of specific structural components of the cells. The technique is advanced by the use of interference filters as well as Spectral Bleed-Through Artifacts (crossover or crosstalk) that is used for broad bandwidths and asymmetrical spectral profiles fluorophores. Laser Systems stimulates and amplifies the molecules with excess energy to emit light. It is the most common light source for scanning confocal fluorescence microscopy.

  • Non-Coherent (non-laser) Light Sources for Confocal Microscopy

This technique use traditional tungsten-halogen source and a short-arc lamp for fluorescence excitation.


Cell Cycle

S-phase stands for synthesis phase